Exostosin-2 antibody
Principal name
Exostosin-2 antibody
Alternative names for Exostosin-2 antibody
EXT2, Multiple exostoses protein 2, Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Putative tumor suppressor protein EXT2
Gene ID
2132 (EXT2)
Ncbi ID
9606, NP_000392, NM_000401, NP_001171554, NP_001171554.1, NM_207122, NM_001178083
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of Exostosin-2 antibody
- Kit Component:
- - KN204067G1, EXT2 gRNA vector 1 in pCas-Guide vector
- KN204067G2, EXT2 gRNA vector 2 in pCas-Guide vector
- KN204067D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Zhao, J., et al. Diabetes 59(3):751-755(2010)
Li, Y., et al. Genet Test Mol Biomarkers 13(6):825-830(2009)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Heinritz, W., et al. Ann. Hum. Genet. 73 (PT 3), 283-291 (2009)
Cornelis, M.C., et al. Ann. Intern. Med. 150(8):541-550(2009).
EXT2 monoclonal antibody (M01), clone 3G6. Western Blot analysis of EXT2 expression in human spleen.
Detection limit for recombinant GST tagged EXT2 is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of EXT2 expression in transfected 293T cell line (H00002132-T01) by EXT2 MaxPab polyclonal antibody.Lane 1: EXT2 transfected lysate(78.98 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of EXT2 expression in transfected 293T cell line (H00002132-T02) by EXT2 MaxPab polyclonal antibody.Lane 1: EXT2 transfected lysate(82.30 KDa).Lane 2: Non-transfected lysate.
Human Hela; Host:Rabbit. Target Name:EXT2. Sample Tissue:Human Hela. Antibody Dilution: 1.0ug/ml. EXT2 is supported by BioGPS gene expression data to be expressed in HeLa.; EXT2 antibody - N-terminal region (ARP44277_P050) in Human Hela cells using Western Blot
Human 721_B; WB Suggested Anti-EXT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate; EXT2 antibody - N-terminal region (ARP44277_P050) in Human 721_B cells using Western Blot
Western blot analysis of lysate from T47D cell line,using EXT2 Antibody (Center)(Cat. #TA325104).TA325104 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of EXT2 Antibody (Center)(Cat#TA325104) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
EXT2 Antibody (Center) (Cat. #TA325104) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-EXT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate
Host: Rabbit; Target Name: EXT2; Sample Tissue: Human Hela; Antibody Dilution: 1.0 ug/mlEXT2 is supported by BioGPS gene expression data to be expressed in HeLa
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA325104 | Exostosin-2 (Center) antibody |
||||||
| Rabbit polyclonal EXT2 Antibody (Center) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN204067 | EXT2 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204067BN | EXT2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204067LP | EXT2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204067RB | EXT2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY424741 | EXT2 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |