FADS2 antibody
Principal name
FADS2 antibody
Alternative names for FADS2 antibody
Fatty acid desaturase 2, D6D, Delta 6 desaturase, Delta(6) fatty acid desaturase
Ncbi ID
9606, NP_004256, NP_004256.1, NP_062673.1, NM_019699, NM_031344, NM_004265
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Western blot / Immunoblot (WB)
Background of FADS2 antibody
- Kit Component:
- - KN305497G1, Fads2 gRNA vector 1 in pCas-Guide vector
- KN305497G2, Fads2 gRNA vector 2 in pCas-Guide vector
- KN305497D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human liver tissue stained with FADS2 Antibody (N-term) Cat.-No AP51507PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of FADS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Flow cytometric analysis of K562 cells using FADS2 Antibody (N-term) Cat.-No AP51507PU-N (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis
Fluorescent image of A549 cell stained with FADS2 Antibody (N-term) Cat.-No AP51507PU-N (Lot SA110322AN). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.
Western blot analysis in HepG2 cell line lysates (35ug/lane) using FADS2 Antibody (N-term) Cat.-No AP51507PU-N. This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).
FADS2 Antibody (Center) (Cat. #TA324808) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).
FADS2 Antibody (Center) (Cat. #TA324808) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP51507PU-N | FADS2 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA324808 | FADS2 antibody |
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| Rabbit polyclonal FADS2 Antibody (Center) | |||||||
 |
Rabbit | IgG | Purified | Bov, Hu, Xen | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP323780 | FADS2 |
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| Recombinant protein of human fatty acid desaturase 2 (FADS2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207091L2V | Lenti ORF particles, Fads2 (GFP-tagged) - Mouse fatty acid desaturase 2 (Fads2), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,100.00
|
|
| OriGene Technologies, Inc. | ||
| MR207091L1V | Lenti ORF particles, Fads2 (Myc-DDK-tagged) - Mouse fatty acid desaturase 2 (Fads2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€990.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213635L2V | Lenti ORF particles, Fads2 (GFP-tagged ORF) - Rat fatty acid desaturase 2 (Fads2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,100.00
|
|
| OriGene Technologies, Inc. | ||
| RR213635L1V | Lenti ORF particles, Fads2 (Myc-DDK-tagged ORF) - Rat fatty acid desaturase 2 (Fads2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€990.00
|
|
| OriGene Technologies, Inc. | ||