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FAK2 / PTK2B antibody

Principal name

FAK2 / PTK2B antibody

Alternative names for FAK2 / PTK2B antibody

PYK2, RAFTK, Protein tyrosine kinase 2 beta, Focal adhesion kinase 2, CAK-beta, CADTK, Proline-rich tyrosine kinase 2, Cell adhesion kinase beta, Calcium-dependent tyrosine kinase, Related adhesion focal tyrosine kinase

SwissProt ID

P70600 (Rat), Q14289 (Human), Q9QVP9 (Mouse)

Gene ID

2185 (PTK2B), 50646 (Ptk2b)

Ncbi ID

NP_004094.3, 9606, NP_004094, AAH36651.1, NM_004103, NM_004103.3, NP_775267.1, AAH36651, NM_173174, NM_173175, NP_775266, NP_775267, NM_017318

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of FAK2 / PTK2B antibody

Protein tyrosine kinase 2 beta, also known as PTK2B, PKB, PTK, CAKB, FAK2, FRNK. Entrez Protein NP_004094. It is a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.

General readings

Ling,S., et al., (2004) Am. J. Physiol., Cell Physiol. 287 (3), C698-C706

Transactivation of PYK2 Whole cell lysates of serum starved HepG2 tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab PYK2-14F6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1:Co; lane 2: serum; lane 3: EGF; lane 4: H2O2;lane 5: Anisomycin; lane 6: Sorbit; lane 7: Arsen; lane 8:Ceramide.

AM00130PU-N

PTK2B monoclonal antibody (M01), clone 1F9 Western Blot analysis of PTK2B expression in K-562 ( Cat # L009V1 ).

H00002185-M01

Western Blot analysis of PTK2B expression in transfected 293T cell line by PTK2B monoclonal antibody (M01), clone 1F9.Lane 1: PTK2B transfected lysate(115.9 KDa).Lane 2: Non-transfected lysate.

H00002185-M01

Detection limit for recombinant GST tagged PTK2B is approximately 0.1ng/ml as a capture antibody.

H00002185-M01

Human Raji; WB Suggested Anti-PTK2B Antibody Titration: 1.25ug/ml. Positive Control: Raji cell lysate. PTK2B is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells.; PTK2B antibody - C-terminal region (ARP32684_T100) in Human Raji cells using Western Blot

ARP32684_T100

Human HepG2; WB Suggested Anti-PTK2B Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; PTK2B antibody - C-terminal region (ARP32685_P050) in Human HepG2 cells using Western Blot

ARP32685_P050

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Pyk2 pTyr402 antibody AP02475PU.

Figure 3. Immunofluorescence staining of methanol-fixed HeLa cells using Pyk2 pTyr402 antibody AP02475PU (Red).

Figure 2. Western blot analysis of extract from Jurkat cells, untreated or treated with PMA (1ng/ml, 5min), using Pyk2 antibody AP02719PU (Lane 1 and 2) and Pyk2 pTyr402 antibody AP02475PU (Lane 3 and 4).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Pyk2 antibody AP02719PU.

Immunofluorescence staining of methanol-fixed HeLa cells using Pyk2 antibody AP02719PU (Red).

Western Blot analysis of extracts from 293 cells using Pyk2 antibody

Immunohistochemistry (IHC) analyzes of PYK2 antibody (Cat.-No.: AP06298PU-N) in paraffin-embedded human brain tissue.

AP06298PU-N

Immunohistochemistry (IHC) analyzes of PYK2 antibody (Cat.-No.: AP06408PU-N) in paraffin-embedded human brain colon carcinoma tissue.

AP06408PU-N

Western blot (WB) analysis of PYK2 antibody (Cat.-No.: AP06408PU-N) in extracts from K562 cells treated with UV 15' or COS7 cells treated with Adriamycin 0.5ng/ml 24h.

AP06408PU-N

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PTK2B polyclonal antibody ( Cat # PAB5540 ) .

PAB5540

Western blot analysis of the extracts from Jurkat cells untreated or treated with PMA using PTK2B polyclonal antibody ( Cat # PAB5540 ) .

PAB5540

PTK2B MaxPab polyclonal antibody. Western Blot analysis of PTK2B expression in rat brain.

H00002185-B01

Western Blot analysis of PTK2B expression in transfected 293T cell line (H00002185-T01) by PTK2B MaxPab polyclonal antibody.Lane 1: PTK2B transfected lysate(110.99 KDa).Lane 2: Non-transfected lysate.

H00002185-B01

Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2B and YES1 Huh7 cells were stained with anti-PTK2B rabbit purified polyclonal 1:1200 and anti-YES1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00002185-D01P

Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2B and ERBB2. HeLa cells were stained with anti-PTK2B rabbit purified polyclonal 1:1200 and anti-ERBB2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00002185-D01P

PTK2B MaxPab rabbit polyclonal antibody. Western Blot analysis of PTK2B expression in rat brain.

H00002185-D01P

Western Blot analysis of PTK2B expression in transfected 293T cell line (H00002185-T01) by PTK2B MaxPab polyclonal antibody.Lane 1: PTK2B transfected lysate(115.90 KDa).Lane 2: Non-transfected lysate.

H00002185-D01P

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)

AP08346PU-N

PTK2B MaxPab polyclonal antibody. Western Blot analysis of PTK2B expression in rat brain.

H00002185-B01P

Western Blot analysis of PTK2B expression in transfected 293T cell line (H00002185-T02) by PTK2B MaxPab polyclonal antibody.Lane 1: PTK2B transfected lysate(110.99 KDa).Lane 2: Non-transfected lysate.

H00002185-B01P

Immunohistochemistry analysis of paraffin-embedded human brain, using PYK2 (Phospho-Tyr881) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from mouse brain, using PYK2 (Phospho-Tyr881) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using PYK2 (Phospho-Tyr580) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from K562 cells, using PYK2 (Phospho-Tyr580) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PYK2 (Phospho-Tyr402) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using PYK2 (Phospho-Tyr579) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells, using PYK2 (Phospho-Tyr579) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PYK2 (Ab-881) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells, treated with Serum 20% 30', using PYK2 (Ab-881) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PYK2 (Ab-580) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS cells, treated with UV 15', using PYK2 (Ab-580) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PYK2 (Ab-402) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells, using PYK2 (Ab-579) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using PYK2 antibody Cat.-No AM06233SU-N

AM06233SU-N

Western blot analysis using PYK2 antibody Cat.-No AM06233SU-N against Raji (Lane 1), PMA induced THP-1 (Lane 2), Jurkat (Lane 3) and Ramos (Lane 4) cell lysate.

AM06233SU-N

Human Colon, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP32225PU-N

Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody AP55794PU-N (left)or the same antibody preincubated with blocking peptide (right).

Western blot analysis of extracts from 3T3 cells (Lane 2) and HepG2 cells (Lane 3), using PYK2 (Phospho-Tyr579) Antibody AP55794PU-N. The lane on the left is treated with antigen-specific peptide.

TP303808

TP305372

TP317684

Western blot analysis of PYK2 expression in Jurkat (A); K562 (B) whole cell lysates.

Immunohistochemical analysis of PYK2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of extracts from 3T3 cells, using PYK2 (Ab-579) antibody.The lane on the right is treated with the synthesized peptide.

TA312567

Western blot analysis of extracts from 3T3 cells (Lane 2), using PYK2 (Ab-579) Antibody. The lane on the left is treated with synthesized peptide.

TA312567

Predicted band size: 116 kDa . Positive control: 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells lysate Lane2: Treated with the peptide Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA321623

Predicted cell location: Cytoplasm; Nucleus; Cell membrane. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using PTK2B (Ab-402) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

TA321623

Predicted cell location: Cytoplasm; Nucleus; Cell membrane. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using PTK2B antibody at dilution 1/100. (Original magnification: ×200)

TA321623

Western blot analysis of extracts from 293 cells, using Phospho-PTK2B-Y402 antibody.

TA333081

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-PTK2B-Y402 antibody.

TA333081

Immunofluorescence staining of methanol-fixed Hela cells using Phospho-PTK2B-Y402 antibody.

TA333081

Predicted band size: 116 kDa . Positive control: 293 cells untreated or treated with Serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with Serum lysate Lane2: 293 cells r treated with Serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA321622

Predicted cell location: Cytoplasm; Nucleus; Cell membrane. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using PTK2B (Phospho-Tyr402) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

TA321622

Predicted cell location: Cytoplasm; Nucleus; Cell membrane. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using PTK2B (Phospho-Tyr402) antibody at dilution 1/100. (Original magnification:×200)

TA321622

Western blot analysis of extracts from 3T3 cells, using PYK2 (Phospho-Tyr579) antibody.The lane on the right is treated with the synthesized peptide.

TA312568

Western blot analysis of extracts from 3T3 cells (Lane 2) and HepG2 cells (Lane 3), using PYK2 (Phospho-Tyr579) Antibody. The lane on the left is treated with synthesized peptide.

TA312568

Immunohistochemistry analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody.The picture on the right is treated with the synthesized peptide.

TA312568

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA312567	FAK2 / PTK2B antibody
	Rabbit polyclonal PYK2 (Ab-579) antibody
	Rabbit	IgG		Purified	Hu, Ms	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA312568	FAK2 / PTK2B antibody
	Rabbit polyclonal PYK2 (Tyr579) antibody(Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA321622	FAK2 / PTK2B antibody
	Anti-PTK2B (Phospho-Tyr402) Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA321623	FAK2 / PTK2B antibody
	Anti-PTK2B Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA333081	FAK2 / PTK2B antibody
	Phospho-PTK2B-Y402 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+2 additional images
AM06233SU-N	FAK2 / PTK2B antibody
	Mouse	IgG2a	5E2D5	Ascites	Hu	E, P, WB	0.1 ml / €420.00
							OriGene Technologies GmbH
+1 additional image
AP02475PU-N	FAK2 / PTK2B pTyr402 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 mg / €300.00
							OriGene Technologies GmbH
+2 additional images
AP02475PU-S	FAK2 / PTK2B pTyr402 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	50 µg / €230.00
							OriGene Technologies GmbH
+2 additional images
AP02719PU-N	FAK2 / PTK2B antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 mg / €300.00
							OriGene Technologies GmbH
+2 additional images
AP02719PU-S	FAK2 / PTK2B antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	50 µg / €230.00
							OriGene Technologies GmbH
+2 additional images
AP06298PU-N	FAK2 / PTK2B antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
AP06408PU-N	FAK2 / PTK2B antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image
AP08346PU-N	FAK2 / PTK2B (N-term) antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms	P, WB	50 µl / €425.00
							OriGene Technologies GmbH
AP32225PU-N	FAK2 / PTK2B antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms, Rt	E, P	50 µl / €425.00
							OriGene Technologies GmbH
AP55794PU-N	FAK2 / PTK2B pTyr579 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €300.00
							OriGene Technologies GmbH
+1 additional image
AP55794PU-S	FAK2 / PTK2B pTyr579 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	50 µg / €230.00
							OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No.	Species	Donor Vector
KN203808	PTK2B - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN203808BN	PTK2B - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN203808LP	PTK2B - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN203808RB	PTK2B - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

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