Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » FANCD2 antibody

FANCD2 antibody

(reset)

Principal name

FANCD2 antibody

Alternative names for FANCD2 antibody

Fanconi anemia group D2 protein, FACD2, FACD

SwissProt ID

Q6IV68 (Rat), Q80V62 (Mouse), Q9BXW9 (Human)

Gene ID

2177 (FANCD2), 211651 (Fancd2)

Ncbi ID

NP_001018125.1, NP_001018125, 9606, NP_149075.2, NP_001028416.2

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of FANCD2 antibody

Fanconi Anemia Complementation Group D2 (FANCD2) protein is one of at least six factors shown to be involved in the autosomal-recessive cancer-prone disorder, Fanconi Anemia (FA). FA group D has been shown to be comprised of two separate proteins, FANCD1 and FANCD2. Mutations in BRCA2 can cause FANCD1. FANCD2 has been shown to colocalize with BRCA1 in ionizing radiation-induced foci. FANCD2 is involved in genomic resistance to DNA cross-linking reagents, and the arrest of DNA synthesis following exposure to ionizing radiation. FANCD2 has been shown to directly interact with NBS1.
FANCD2 is one of at least four different genes, of which when mutated can cause the Fanconi anemia phenotype characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomcyin C. FANCD2 is phosphorylated by ATM following ionizing radiation and this phosphorylation is required for activation of an S-phase checkpoint. It is also monoubiquitinated in response to DNA damage. It is postulated that FANCD2 may have a role in the cellular response to stalled replication forks or in the repair of replication-associated double-strand breaks.

AP06115PU-N - FANCD2
Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using FANCD2  antibody (Cat.-No. AP06115PU-N).
AP06115PU-N - FANCD2
Western blot (WB) analysis of FANCD2 antibody (Cat.-No.: AP06115PU-N) at 1/500 dilution
Lane1:Hela cell lysate
Lane2:SP2/0 cell lysate
Lane3:Mouse liver tissue lysate
AM06065PU-N - FANCD2
Formalin-Fixed Paraffin-Embedded Human Liver stained with FANCD2 antibody AM06065PU-N at 2.5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AM06066PU-N - FANCD2
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
MAB2398 - FANCD2
Expression of FancD2 ( mAb ) in different cell lines : proliferating cells were treated with IR or kept as mock of IR. Eight hours after IR, local proteins were isolated for Western blot analysis. Fifty microgram protein was loaded each lane. Working dilution of FANCD2 monoclonal antibody, clone FI-17 ( Cat # MAB2398 ) is 1 : 1000. Lane1 : U2OS, Lane2 : U2OS+IR, Lane3 and 4 : MEF, Lane5 : MO59K+IR, Lane6 : SiHa+IR.
PAB12428 - FANCD2
Staining of human prostate, glandular epithelium using FANCD2 polyclonal antibody ( Cat # PAB12428 ).
PAB12428 - FANCD2
FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. FANCD2 were stained with FANCD2 polyclonal antibody ( Cat # PAB12428 ).
PAB12428 - FANCD2
FANCD2 polyclonal antibody ( Cat # PAB12428 ) was used to stain FANCD2. FANCD2 colocalizes in vivo with another protein in U-2 OS cells after cell exposure to IR. Proliferating U-2 OS cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h.
PAB12428 - FANCD2
Western blot analysis of FANCD2 in HeLa whole cell extract using FANCD2 polyclonal antibody ( Cat # PAB12428 ).
PAB12530 - FANCD2
FA-D2 fibroblasts were stably transfected with either pMMP ( empty vector, A ), FANCD2 ( wt, B ), FANCD2 ( S222A, C ), FANCD2 ( triple mutant, D ), or FANCD2 ( quadruple mutant, E ). Cells were irradiated with 15 Gy ( + ), and whole cell lysates were immunoblotted with either anti-FANCD2 or Phospho-FANCD2 S222 polyclonal antibody, ( Cat # PAB12530 ).
PAB12530 - FANCD2
Indicated lymphoblasts ( PD7, WT : GM1526, AT ) were irradiated with 15 Gy ( 2 ), and immunoblotted with anti-FANCD2 and FANCD2 (phospho S222) polyclonal antibody ( Cat # PAB12530 ).
A0427-1 - FANCD2
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using FANCD2 (Phospho-Ser222) Antibody. The picture on the right is treated with the synthesized peptide.
A0427-1 - FANCD2
Western blot analysis of extracts from HT29 cells treated with Calyculin A 50ng/ml 30', using FANCD2 (Phospho-Ser222) Antibody. The lane on the right is treated with the synthesized peptide.
B0427-1 - FANCD2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FANCD2 (Ab-222) Antibody. The picture on the right is treated with the synthesized peptide.
B0427-1 - FANCD2
Western blot analysis of extracts from HT-29 cells, treated with Calyculin A 50ng/ml 30', using FANCD2 (Ab-222) Antibody. The lane on the right is treated with the synthesized peptide.
AP55916PU-S - FANCD2
Western blot analysis of extracts from HT29 cells treated with CalyculinA using Phospho-FANCD2 (Ser222) antibody AP55916PU-N.The lane on the right is treated with the antigen-specific peptide.
TA590428 - FANCD2
ELISA: FANCD2 Antibody
TA309656 - FANCD2
Western Blot: FANCD2 Antibody (FI-17) - Expression of FancD2 (mAb) in different cell lines. Proliferating cells were treated with 10-Gy IR. Fifty microgram protein was loaded each lane. Working dilution - 1:1000 LCL 6% SDS-PAGE.
TA309656 - FANCD2
Western Blot: FANCD2 Antibody (FI-17) - Western blot of FANCD2 on MCF7 cell line (lane 1) and HeLa whole cell extract (lane 2) using anti-FANCD2.
TA313866 - FANCD2
Western blot analysis of extracts from JK cells, using FANCD2 (Ab-691) antibody.The lane on the right is treated with the synthesized peptide.
TA323418 - FANCD2
Predicted band size: 166 kDa . Positive control: HL-60 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA325458 - FANCD2
Western blot analysis of FANCD2 expression in CalyculinA treated HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA327255 - FANCD2
Western blot analysis of extracts of various cell lines, using FANCD2 antibody.
TA327255 - FANCD2
Immunofluorescence analysis of MCF7 cell using FANCD2 antibody. Blue: DAPI for nuclear staining.
TA336272 - FANCD2
Western Blot: FANCD2 Antibody [TA336272] - Analysis of FANCD2 in human PD20 cells. Image courtesy of anonymous customer review
TA336272 - FANCD2
Western Blot: FANCD2 Antibody [TA336272] - FANCD2 in HeLa WCE using NB 100-182 (lot C).
TA336272 - FANCD2
Immunohistochemistry: FANCD2 Antibody [TA336272] - Staining of human prostate, glandular epithelium.
TA336272 - FANCD2
Immunocytochemistry/Immunofluorescence: FANCD2 Antibody [TA336272] - FancD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence stainin
TA336272 - FANCD2
Immunocytochemistry/Immunofluorescence: FANCD2 Antibody [TA336272] - FancD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to IR. Proliferating U2OS cells were exposed to 10 Gy of IR and double -color immunofluorescence stainin
TA336310 - FANCD2
Western Blot: FANCD2 [p Ser222] Antibody [TA336310] - FA-D2 fibroblasts were stably transfected with either pMMP (empty vector, A), FANCD2 (wt, B), FANCD2 (S222A, C), FANCD2 (triple mutant, D), or FANCD2 (quadruple mutant, E).
TA336310 - FANCD2
Western Blot: FANCD2 [p Ser222] Antibody [TA336310] - Indicated lymphoblasts (PD7, WT: GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and anti-FANCD2 (pS-222)
TA336730 - FANCD2
Simple Western: FANCD2 Antibody [TA336730] - Simple Western lane view shows a specific band detected for FANCD2 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).
TA336730 - FANCD2
Western Blot: FANCD2 Antibody [TA336730] - Cell lysates of wildtype lymphoblasts (HSC93) and fibroblasts (LN9SV) and FANCD2 mutated lymphoblasts (VU1288) and (PD20) probed with anti-FANCD2 antibody showing clear FANCD2 protein bands in the wild-type cells
TA336730 - FANCD2
Western Blot: FANCD2 Antibody [TA336730] - Immunofluorescence with anti-FANCD2, polyclonal(A-C) triple staining of the adult stomach with DAPI(blue), anti-FANCD2(red), and anti Ki-67 (green), (D) Merged image of FANCD2 and Ki-67 staining showing co-expres
TA336730 - FANCD2
Immunohistochemistry: FANCD2 Antibody [TA336730] - Immunohistochemical analysis of FANCD2 expression in FA cell lines with anti-FANCD2, polyclonal FANCD2-expressing cells exhibit dark brown nuclear staining: cell types are specified. Negative controls wit
TA325457 - FANCD2
Western blot analysis of FANCD2 phosphorylation expression in CalyculinA treated HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
  1. Quick-Filters:
  2. Highly Validated (1)

13 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FANCD2 antibody
  Rabbit Polyclonal FANCD2 Antibody  
Western Blot: FANCD2 Antibody [TA336272] - Analysis of FANCD2 in human PD20 cells. Image courtesy of anonymous customer review Rabbit Purified Hu, Ms, Prim C, ICC/IF, IP, P, WB
50 µl / €475.00
  OriGene Technologies, Inc.
+4 additional images

Highly validated antibody!

FANCD2 antibody
  Mouse Monoclonal FANCD2 Antibody (FI-17)  
Western Blot: FANCD2 Antibody (FI-17) - Expression of FancD2 (mAb) in different cell lines. Proliferating cells were treated with 10-Gy IR. Fifty microgram protein was loaded each lane. Working dilution - 1:1000 LCL 6% SDS-PAGE. Mouse FI-17 Purified Hu IP, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

FANCD2 antibody
  Rabbit polyclonal FANCD2 (Ab-691) antibody  
Western blot analysis of extracts from JK cells, using FANCD2 (Ab-691) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

FANCD2 antibody
  Anti-FANCD2 Rabbit Polyclonal Antibody  
Predicted band size: 166 kDa . Positive control: HL-60 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

FANCD2 antibody
  Rabbit Polyclonal FANCD2 (Ser222) Antibody (Phospho-specific)  
Western blot analysis of FANCD2 phosphorylation expression in CalyculinA treated HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

FANCD2 antibody
  Rabbit Polyclonal FANCD2 Antibody  
Western blot analysis of FANCD2 expression in CalyculinA treated HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

FANCD2 antibody
  FANCD2 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using FANCD2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

FANCD2 antibody
  Rabbit Polyclonal FANCD2 [p Ser222] Antibody  
Western Blot: FANCD2 [p Ser222] Antibody [TA336310] - FA-D2 fibroblasts were stably transfected with either pMMP (empty vector, A), FANCD2 (wt, B), FANCD2 (S222A, C), FANCD2 (triple mutant, D), or FANCD2 (quadruple mutant, E). Rabbit Purified Hu WB
0.2 ml / €485.00
  OriGene Technologies, Inc.
+1 additional image

FANCD2 antibody
Formalin-Fixed Paraffin-Embedded Human Liver stained with FANCD2 antibody AM06065PU-N at 2.5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG 103 Purified Hu E, P, WB
50 µl / €425.00
  OriGene Technologies GmbH

FANCD2 antibody
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG 6 Purified Hu P, WB
50 µl / €425.00
  OriGene Technologies GmbH

FANCD2 antibody
Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using FANCD2  antibody (Cat.-No. AP06115PU-N). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

FANCD2 pSer222 antibody
Western blot analysis of extracts from HT29 cells treated with CalyculinA using Phospho-FANCD2 (Ser222) antibody AP55916PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €300.00
  OriGene Technologies GmbH

FANCD2 pSer222 antibody
Western blot analysis of extracts from HT29 cells treated with CalyculinA using Phospho-FANCD2 (Ser222) antibody AP55916PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €230.00
  OriGene Technologies GmbH

13 Item(s)

per page
13 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (13)
Clonality
  1. Polyclonal (10)
  2. Monoclonal (3)
Target
  1. FANCD2 (13)
Property
  1. pSer222 (2)
Reactivity
  1. Human (Hu) (13)
  2. Mouse (Ms) (8)
  3. Rat (Rt) (7)
  4. Primate (Prim) (1)
Applications
  1. Western blot / Immunoblot (WB) (12)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (2)
  4. Immunoprecipitation (IP) (2)
  5. Frozen sections (C) (1)
  6. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (10)
  2. Aff - Purified (3)
Host
  1. Rabbit (10)
  2. Mouse (3)
Images
  1. 1 image (13)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (11)
  2. Paraffin Sections (P) (3)
  3. Immunofluorescence (IF) (2)
  4. IHC (1)
Manufacturer
  1. OriGene Technologies (13)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn