Fascin antibody
Principal name
Fascin antibody
Alternative names for Fascin antibody
SCN1, FAN1, HSN, SNL, p55, Singed-like protein, Dendritic cell marker, 55 kDa actin-bundling protein
Ncbi ID
NP_005219, NP_003079, NM_007984, NP_003079.1, NP_032010.2, NM_003088, NM_001100806
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Fascin antibody
Fascin is a actin cross-linking protein.The Fascin gene contains 5 exons and spans 7 kb. It is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated from sea urchin egg but also found in Drosophila and vertebrates, including humans. Fascin (from the Latin for bundle) is spaced at 11 nanometre intervals along the filament. The bundles in cross section are seen to be hexagonally packed, and the longitudinal spacing is compatible with a model where fascin cross-links at alternating 4 and 5 actins. It is calcium insensitive and monomeric. Fascin binds beta-catenin, and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of Fascin in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. Abnormal fascin expression or function has been implicated in breast cancer, colon cancer, esophageal squamous cell carcinoma, gallbladder cancer and prostate cancer.
General readings
Wu D., et al. (2010) Ann Oncol. 21(3): 589-596. Shin H., et al. (2009) Phys Rev Left. 103(23): 238102.
Formalin-Fixed, Paraffin-Embedded Human Hodgkin’s lymphoma stained with Fascin antibody Cat.-No. AM10085SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of Hodgkin's cells.
Western blot analysis of Fascin Antibody (Center) (AP17358PU-N) in Y79 cell line lysates (35 µg/lane). Fascin (arrow) was detected using the purified Pab.
Recombinant human FSCN1, 1-493aa, His-tagged
Mouse Thymus; WB Suggested Anti-Fscn1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Thymus; Fscn1 antibody - N-terminal region (ARP46088_P050) in Mouse Thymus cells using Western Blot
AP32962PU-N (1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807293)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807293)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807293)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807293)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807293)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807293)(1:150)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807293)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FSCN1 (RC203031, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FSCN1.(1:500)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807305)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807305)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807305)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FSCN1 (RC203031, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FSCN1.(1:2000)
Sample (30 ug of whole cell lysate). A: HeLa. B: Hep G2 . 7.5% SDS PAGE. TA308801 diluted at 1:1000
Immunohistochemistry staining of Paraffin Hodgkin lymphoma tissue by Fascin antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic)
WB Suggested Anti-Fscn1 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Thymus
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FSCN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : THP-1 cell lysate
ICC/IF analysis of FSCN1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FSCN1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Raji cells, U937 cells, Primary antibody: (FSCN1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (FSCN1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using (FSCN1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA807293 | Fascin antibody |
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| FSCN1 mouse monoclonal antibody, clone OTI3B4 (formerly 3B4) | |||||||
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Mouse | IgG1 | OTI3B4 | Purified | Hu | P, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
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| TA807305 | Fascin antibody |
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| FSCN1 mouse monoclonal antibody, clone OTI5G1 (formerly 5G1) | |||||||
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Mouse | IgG2a | OTI5G1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| AP32962PU-N | Fascin antibody |
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Goat | Aff - Purified | Bov, Hu, Ms, Rt | WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA308801 | Fascin antibody |
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| Rabbit polyclonal antibody to Fascin (fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327676 | Fascin antibody |
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| Mouse Monoclonal Fascin Antibody | |||||||
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Mouse | IgG1 | 55-k2 | Purified | Hu | P |
1 ml /
€705.00
|
| OriGene Technologies, Inc. | |||||||
| TA349243 | Fascin antibody |
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| Rabbit Polyclonal Anti-FSCN1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA349244 | Fascin antibody |
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| Rabbit Polyclonal Anti-FSCN1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM10085SU-N | Fascin antibody |
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Mouse | IgG1 | 55K-2 | Supernatant | Hu, Rt | P |
1 ml /
€540.00
|
| OriGene Technologies GmbH | |||||||
| AM50621PU-N | Fascin antibody |
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Mouse | IgG2b | AT13D2 | Purified | Hu | E, F, ICC/IF, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AM50621PU-S | Fascin antibody |
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|
Mouse | IgG2b | AT13D2 | Purified | Hu | E, F, ICC/IF, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP17358PU-N | Fascin antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| AP54932SU-N | Fascin pSer39 antibody |
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Rabbit | Serum | Hu | C, E, WB |
0.2 ml /
€430.00
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303031 | Fascin |
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| Recombinant protein of human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214769L2V | Lenti ORF particles, LOC683788 (GFP-tagged ORF) - Rat similar to Fascin (Singed-like protein) (LOC683788), 200 uL, >10^7 TU/mL |
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200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||
| RR214769L1V | Lenti ORF particles, LOC683788 (Myc-DDK-tagged ORF) - Rat similar to Fascin (Singed-like protein) (LOC683788), 200 uL, >10^7 TU/mL |
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200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||
