FAST kinase antibody
Principal name
FAST kinase antibody
Alternative names for FAST kinase antibody
FASTK, Fas-activated serine/threonine kinase
Gene ID
10922 (FASTK)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of FAST kinase antibody
- Kit Component:
- - KN204063G1, FASTK gRNA vector 1 in pCas-Guide vector
- KN204063G2, FASTK gRNA vector 2 in pCas-Guide vector
- KN204063D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Simarro, M., et al. J. Immunol. 184(9):5325-5332(2010)
de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009)
Simarro, M., et al. Proc. Natl. Acad. Sci. U.S.A. 104(27):11370-11375(2007)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Izquierdo, J.M., et al. J. Biol. Chem. 282(3):1539-1543(2007).
The FASTK polyclonal antibody ( Cat # PAB4642 ) is used in Western blot to detect FASTK in HL-60 cell lysate .
Western Blot analysis of FASTK expression in transfected 293T cell line by FASTK monoclonal antibody (M02), clone 2B7.Lane 1: FASTK transfected lysate(61.104 KDa).Lane 2: Non-transfected lysate.
The anti-FASTK Pab is used in Western blot to detect FASTK in HL-60 cell lysate.
Western blot (WB) analysis of FAST antibody (Cat.-No.: AP20588PU-N) in extracts from pc12 cells.
Formalin fixed and paraffin embedded human liver tissue reacted with FAST kinase (C-term) Antibody (C-term) Cat.-No followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of FASTK (arrow) using FAST kinase (C-term) Antibody (C-term) Cat.-No AP51612PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FASTK gene.
Immunofluorescent analysis of FAST Kinase staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of FAST Kinase expression in PC12 (A); HL60 (B) whole cell lysates.
FAST antibody detects FASTK protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug A375 whole cell lys
Sample (30 ug of whole cell lysate). . A:NIH-3T3. 7.5% SDS PAGE. . TA308720 diluted at 1:1000.
FAST antibody detects FASTK protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. FAST antibody (TA308720) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using FAST(TA308720) antibody at 1:100 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using FAST (TA308720) antibody at 1:200 dilution.
FAST antibody detects FAST protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FAST protein stained by FAST antibody (TA308720) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar
The image is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (FASTK Antibody) at dilution 1/30. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human brain tissue using (FASTK Antibody) at dilution 1/30. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308720 | FAST kinase antibody |
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| Rabbit Polyclonal antibody to FAST (Fas-activated serine/threonine kinase) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody! |
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| TA349317 | FAST kinase antibody |
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| Rabbit Polyclonal Anti-FASTK Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP13667PU-N | FAST kinase (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP20588PU-N | FAST kinase antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP51612PU-N | FAST kinase (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307877 | FAST kinase (transcript variant 4) |
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| Recombinant protein of human Fas-activated serine/threonine kinase (FASTK), transcript variant 4 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||