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FBXO4 antibody

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Principal name

FBXO4 antibody

Alternative names for FBXO4 antibody

F-box only protein 4, FBX4

SwissProt ID

Q3T0J1 (Bovin), Q8CHQ0 (Mouse), Q9UKT5 (Human)

Gene ID

26272 (FBXO4)

Ncbi ID

9606, NP_036308, NP_277019, NP_036308.1, 10090, NM_012176, NM_033484

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of FBXO4 antibody

FBXO4 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXO4 belongs to the Fbxs class.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants.

General readings

Zeng,Z., et.al., Dev. Cell 18 (2), 214-225 (2010)
Barbash,O., et.al., Cancer Cell 14 (1), 68-78 (2008)
Sugiyama,N., et.al., Mol. Cell Proteomics 6 (6), 1103-1109 (2007).

H00026272-B01 - FBXO4
Western Blot analysis of FBXO4 expression in transfected 293T cell line (H00026272-T01) by FBXO4 MaxPab polyclonal antibody.Lane 1: FBXO4 transfected lysate(42.57 KDa).Lane 2: Non-transfected lysate.
H00026272-B01P - FBXO4
Western Blot analysis of FBXO4 expression in transfected 293T cell line (H00026272-T01) by FBXO4 MaxPab polyclonal antibody.Lane 1: FBXO4 transfected lysate(42.57 KDa).Lane 2: Non-transfected lysate.
H00026272-D01P - FBXO4
FBXO4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FBXO4 expression in human pancreas.
H00026272-D01P - FBXO4
Western Blot analysis of FBXO4 expression in transfected 293T cell line (H00026272-T02) by FBXO4 MaxPab polyclonal antibody.Lane 1: FBXO4 transfected lysate(44.10 KDa).Lane 2: Non-transfected lysate.
PAB10205 - FBXO4
Immunofluorescence staining of NIH/3T3 cells with FBX4 polyclonal antibody ( Cat # PAB10205 ) . Cells were fixed in methanol/acetone followed by incubation with FBX4 polyclonal antibody at a 1 : 300 dilution.Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
PAB10205 - FBXO4
Whole cell extracts prepared from U-2 OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose.FBX4 was detected using FBX4 polyclonal antibody ( Cat # PAB10205 ) at a 1:500 dilution, in TBS buffer containing 0.1% Tween-20, followed by peroxidase conjugated anti-rabbit IgG.Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
ARP43122_P050 - FBXO4
Human 293T; WB Suggested Anti-FBXO4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; FBXO4 antibody - middle region (ARP43122_P050) in Human 293T cells using Western Blot
H00026272-D01 - FBXO4
FBXO4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FBXO4 expression in human pancreas.
H00026272-D01 - FBXO4
Immunoprecipitation of FBXO4 transfected lysate using anti-FBXO4 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with FBXO4 MaxPab mouse polyclonal antibody (B01) (H00026272-B01).
ARP43123_P050 - FBXO4
Human OVCAR-3; WB Suggested Anti-FBXO4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: OVCAR-3 cell lysate. FBXO4 is supported by BioGPS gene expression data to be expressed in OVCAR3.; FBXO4 antibody - middle region (ARP43123_P050) in Human OVCAR-3 cells using Western Blot
AP51631PU-N - FBXO4
Western blot analysis of FBXO4 Antibody (Center) Cat.-No AP51631PU-N in Y79 cell line lysates (35ug/lane). FBXO4 (arrow) was detected using the purified Pab.
TP308458 - FBXO4
TA319513 - FBXO4
Whole cell extracts prepared from U2OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose. FBX4 was detected using Rockland’s anti-FBX4 at a 1:500 dilution, in TBS buffer containing 0.1% Tween-20, followed by peroxidase conjugated anti-rabbit IgG. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
TA319513 - FBXO4
Immunocytochemistry staining of NIH-3T3 cells with Rockland’s anti-FBX4. Cells were fixed in methanol/acetone followed by incubation with anti-FBX4 at a 1:300 dilution. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia,
TA326314 - FBXO4
TA326314 (0.5µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA329824 - FBXO4
WB Suggested Anti-FBXO4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate
TA329825 - FBXO4
WB Suggested Anti-FBXO4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: OVCAR-3 cell lysateFBXO4 is supported by BioGPS gene expression data to be expressed in OVCAR3

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBXO4 (N-term) antibody
  Rabbit polyclonal anti-FBXO4 antibody  
Whole cell extracts prepared from U2OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose. FBX4 was detected using Rockland’s anti-FBX4 at a 1:500 dilution, in TBS buffer containing 0.1% Tween-20, followed by peroxidase conjugated anti-rabbit IgG. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA. Rabbit IgG Serum Hu, Ms ICC/IF, WB
0.1 ml / €515.00
  OriGene Technologies, Inc.
+1 additional image

FBXO4 antibody
  Goat Anti-FBXO4 Antibody  
TA326314 (0.5µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

FBXO4 (Center) antibody
Western blot analysis of FBXO4 Antibody (Center) Cat.-No AP51631PU-N in Y79 cell line lysates (35ug/lane). FBXO4 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FBXO4 (transcript variant 1)
  Recombinant protein of human F-box protein 4 (FBXO4), transcript variant 1  
FBXO4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

4 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (3)
  2. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (3)
Target
  1. FBXO4 (4)
Property
  1. Center (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Bovine (Bov) (1)
  4. Canine (Can) (1)
  5. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (2)
  2. Serum (1)
Host
  1. Rabbit (2)
  2. Goat (1)
Images
  1. 1 image (4)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (4)
Service
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