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FBXO43 antibody

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Principal name

FBXO43 antibody

Alternative names for FBXO43 antibody

F-box only protein 43, EMI2, FBP5B, Endogenous meiotic inhibitor 2

SwissProt ID

Q4G163 (Human), Q66H04 (Rat), Q8AXF4 (Xenla), Q8CDI2 (Mouse)

Gene ID

286151 (FBXO43), 100101275 (fbxo43)

Ncbi ID

NP_001025031.2, 9606, NP_001093338.1, XP_209918, NM_001029860, AF029213

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of FBXO43 antibody

Kit Component:
- KN216347G1, FBXO43 gRNA vector 1 in pCas-Guide vector
- KN216347G2, FBXO43 gRNA vector 2 in pCas-Guide vector
- KN216347D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 1997;388:548-54
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an IkappaB kinase. Cell 1997;90:373-83
3. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell 1997;91:243-52
Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB kinase-alpha: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK. Science 1997;278:866-9

AP07584PU-N - FBXO43
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver.
PAB10206 - FBXO43
Western blot using FBXO43 polyclonal antibody ( Cat # PAB10206 ).Panels A and B show two identical membranes containing marker, in vitro translated FBXO43 ( FBXO43 ivt ), and a whole cell extract from 293T cells loaded in duplicate.Panel A shows antibody reactivity.Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide.This pre-incubation greatly diminishes specific band recognition.Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells.When this experiment was repeated using a mock in vitro translation, no band was detected ( data not shown ).Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.
TA306000 - FBXO43
Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody (C2) at 1:1000 dilution.
TA306001 - FBXO43
Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody at 1:500 dilution.
TA306001 - FBXO43
Immunocytochemistry of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 ug/mL.
TA308747 - FBXO43
Sample (30 ug of whole cell lysate). A: NIH-3T3. B: JC. 7.5% SDS PAGE. TA308747 diluted at 1:1000.
TA308747 - FBXO43
Sample (30 ug of whole cell lysate). A: 293T. 7.5% SDS PAGE. TA308747 diluted at 1:1000.
TA308747 - FBXO43
Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using FBXO43 (TA308747) antibody at 1:100 dilution.
TA308747 - FBXO43
Immunofluorescence analysis of paraformaldehyde-fixed A431, using FBXO43(TA308747) antibody at 1:200 dilution.
TA319329 - FBXO43
WB using Anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells.
  1. Quick-Filters:
  2. Highly Validated (1)

7 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBXO43 antibody
  Rabbit Polyclonal antibody to FBXO43 (F-box protein 43)  
Sample (30 ug of whole cell lysate). A: NIH-3T3. B: JC. 7.5% SDS PAGE. TA308747 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

FBXO43 (C-term) antibody
  Goat Anti-EMI2 Antibody  
FBXO43 Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, F, ICC/IF, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

FBXO43 antibody
  Rabbit Polyclonal IL-1RAcP Antibody  
Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody (C2) at 1:1000 dilution. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.

FBXO43 antibody
  Rabbit Polyclonal IL-1RAcP Antibody  
Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

FBXO43 antibody
  Rabbit polyclonal anti-Fbp5B antibody  
WB using Anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. Rabbit IgG Aff - Purified Chimp, Hu E, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

FBXO43 (N-term) antibody
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

FBXO43 overexpression lysate
FBXO43 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

7 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (6)
Clonality
  1. Polyclonal (6)
Target
  1. FBXO43 (6)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (2)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
  6. Chimpanzee (Chimp) (1)
  7. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Enzyme Immunoassay (E) (5)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (4)
  4. Paraffin Sections (P) (2)
  5. Flow cytometry (F) (1)
Presentation
  1. Aff - Purified (6)
Host
  1. Rabbit (5)
  2. Goat (1)
Images
  1. 1 image (5)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunofluorescence (IF) (2)
  3. IHC (1)
  4. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (7)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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