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FBXO5 antibody

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Principal name

FBXO5 antibody

Alternative names for FBXO5 antibody

F-box only protein 5, EMI1, FBX5, Early mitotic inhibitor 1

SwissProt ID

Q7TSG3 (Mouse), Q9UKT4 (Human)

Gene ID

26271 (FBXO5), 67141 (Fbxo5)

Ncbi ID

9606, NP_036309, NP_001135994, NM_012177, NP_001135994.1, NP_080271.2, NM_025995, NM_001142522

Available reactivities

Hu (Human), Chimp (Chimpanzee), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of FBXO5 antibody

FBXO5 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitition. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein belongs to the Fbxs class and is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the aphase promoting complex. Altertively spliced transcript variants encoding different isoforms have been identified.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitition. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the aphase promoting complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

Lei, S.F., et al. J. Bone Miner. Res. (2010) In press.
Ma, H.T., et al. Mol. Cell. Biol. 29(24):6500-6514(2009).
Lee, J., et al. Mol. Biol. Cell 20(7):1891-1902(2009)
Gutgemann, I., et al. Mod. Pathol. 21(4):445-454(2008)
Iwai, H., et al. Cell 130(4):611-623(2007).

PAB10207 - FBXO5
Immunohistochemistry of FBXO5 polyclonal antibody ( Cat # PAB10207 ) shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue.Tissue was formalin-fixed and paraffin embedded.Brown color indicates presence of protein, blue color shows cell nuclei.Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
PAB10207 - FBXO5
Western blot using FBXO5 polyclonal antibody ( Cat # PAB10207 ) shows detection of a major band corresponding to FBXO5 protein in a human HeLa whole cell lysate ( lane 1 arrowhead ).The identity of cross-reactive minor bands at ~72 kDa and 150 kDa is unknown.Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide ( lane 2 ).Approximately 33 µg of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose.The membrane was blocked using BLOTTO.Primary antibody was used at a 1:500 dilution in BLOTTO.The membrane was washed and reacted with a 1:10,000 dilution of IRDyeTM800 conjugated affinity purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green ).Molecular weight estimation was made by comparison to prestained MW markers indicated at the right ( lane M, 700 nm channel, red ).
ARP43125_P050 - FBXO5
Hum. Adult Placenta; Host:Rabbit. Target Name:FBXO5. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; FBXO5 antibody - C-terminal region (ARP43125_P050) in Hum. Adult Placenta cells using Western Blot
ARP43125_P050 - FBXO5
Hum. Adult Placenta; Host:Rabbit. Target Name:FBXO5. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; FBXO5 antibody - C-terminal region (ARP43125_P050) in Hum. Adult Placenta cells using Western Blot
ARP43125_P050 - FBXO5
Hum. Fetal Lung; Host:Rabbit. Target Name:FBXO5. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; FBXO5 antibody - C-terminal region (ARP43125_P050) in Hum. Fetal Lung cells using Western Blot
ARP43125_P050 - FBXO5
Human HepG2; WB Suggested Anti-FBXO5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; FBXO5 antibody - C-terminal region (ARP43125_P050) in Human HepG2 cells using Western Blot
AP51634PU-N - FBXO5
Western blot analysis of FBXO5 Antibody (Center) Cat.-No AP51634PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the FBXO5 antibody detected the FBXO5 protein (arrow).
TA319364 - FBXO5
WB using Anti-Fbp5A antibody shows detection of a major band corresponding to Fbp5A protein in a human HeLa whole cell lysate (lane 1 arrowhead). The identity of cross-reactive minor bands at ~72 kDa and 150 kDa is unknown. Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Primary antibody was used at 1:500. Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red).
TA319364 - FBXO5
Affinity Purified anti-Fbp5a antibody shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue.  Tissue was formalin-fixed and paraffin embedded.  Brown color indicates presence of protein, blue color shows cell nuclei.   Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
TA329826 - FBXO5
WB Suggested Anti-FBXO5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate
TA329826 - FBXO5
Host:Rabbit; Target Name:FBXO5; Sample Tissue:Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
TA329826 - FBXO5
Host:Rabbit; Target Name:FBXO5; Sample Tissue:Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
TA329826 - FBXO5
Host:Rabbit; Target Name:FBXO5; Sample Tissue:Human Adult Placenta; Antibody Dilution: 1.0 ug/ml

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBXO5 antibody
  Rabbit polyclonal anti-Fbp5A antibody  
WB using Anti-Fbp5A antibody shows detection of a major band corresponding to Fbp5A protein in a human HeLa whole cell lysate (lane 1 arrowhead). The identity of cross-reactive minor bands at ~72 kDa and 150 kDa is unknown. Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Primary antibody was used at 1:500. Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.
+1 additional image

FBXO5 (Center) antibody
Western blot analysis of FBXO5 Antibody (Center) Cat.-No AP51634PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the FBXO5 antibody detected the FBXO5 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

FBXO5 overexpression lysate
FBXO5 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Fbxo5 (GFP-tagged) - Mouse F-box protein 5 (Fbxo5), 200 uL, >10^7 TU/mL
Lenti ORF particles, Fbxo5 (GFP-tagged) - Mouse F-box protein 5 (Fbxo5), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Fbxo5 (Myc-DDK-tagged) - Mouse F-box protein 5 (Fbxo5), 200 uL, >10^7 TU/mL
Lenti ORF particles, Fbxo5 (Myc-DDK-tagged) - Mouse F-box protein 5 (Fbxo5), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

5 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (2)
Clonality
  1. Polyclonal (2)
Target
  1. FBXO5 (2)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (2)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Host
  1. Rabbit (2)
Images
  1. 1 image (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. IHC (1)
Manufacturer
  1. OriGene Technologies (5)
Service
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