FCER1A antibody
Principal name
FCER1A antibody
Alternative names for FCER1A antibody
IgE Fc receptor subunit alpha, Fc-epsilon RI-alpha, FCE1A, FcERI, High affinity immunoglobulin epsilon receptor subunit alpha
Ncbi ID
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Flow Cytometry (F), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Background of FCER1A antibody
- Kit Component:
- - KN203321G1, FCER1A gRNA vector 1 in pCas-Guide vector
- KN203321G2, FCER1A gRNA vector 2 in pCas-Guide vector
- KN203321D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
ZHANG,Y., et al. (2012) Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 47 (4), 289-293 Zhou,J., et al. (2012) Hum. Immunol. 73 (3), 301-305
IHC Image of AM20559PU-NMast Cell in Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Staining of human lysed whole blood with Purified Mouse IgG2b isotype control (green histogram) or Purified AER-37 (CRA1) (blue histogram) followed by Biotin Anti-Mouse IgG and Streptavidin-PE. Cells in the lymphocyte gate were used for analysis.
Human Muscle; WB Suggested Anti-FCER1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; FCER1A antibody - middle region (ARP45205_P050) in Human Muscle cells using Western Blot
FCER1A MaxPab rabbit polyclonal antibody. Western Blot analysis of FCER1A expression in human kidney.
Western Blot analysis of FCER1A expression in transfected 293T cell line (H00002205-T02) by FCER1A MaxPab polyclonal antibody.Lane 1: FCER1A transfected lysate(29.60 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi ( Cat # H00002205-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FCER1A monoclonal antibody (M01), clone 2C12-3B6 (Cat # H00002205-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody (M01), clone 2C12-3B6.Lane 1: FCER1A transfected lysate(29.6 KDa).Lane 2: Non-transfected lysate.
FCER1A monoclonal antibody (M01), clone 2C12-3B6 Western Blot analysis of FCER1A expression in A-549 ( Cat # L025V1 ).
Immunohistochemistry: SM2251P FCER1A antibody staining of Formalin-Fixed, Paraffin-Embedded Human Mast Cells at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Flow Cytometry: SM2251P FCER1A antibody staining of Human peripheral blood cells with Mouse anti Human FC Epsilon R1 showing staining of eosinophils.
Western blot analysis of extracts of mouse spleen cell lines, using FCER1A antibody.
TA334189 (0.3µg/ml) staining of Human Peripheral Blood Lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 30 kDa . Positive control: Mouse spleen tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA321419 | FCER1A antibody |
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| Anti-FCER1A Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327164 | FCER1A antibody |
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| Rabbit anti-FCER1A Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA334189 | FCER1A (177-189) antibody |
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| Goat Anti-FCER1A (aa177-189) Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| AM20559PU-N | FCER1A antibody |
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 |
Mouse | IgG2b | AER-37 (CRA1) | Aff - Purified | Hu | F, P |
50 µg /
€425.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| SM2251P | FCER1A antibody |
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 |
Mouse | IgG2b | 9E1 | Purified | Hu | E, F, ICC/IF, IP, P, WB |
0.2 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| SM2251PS | FCER1A antibody |
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 |
Mouse | IgG2b | 9E1 | Purified | Hu | E, F, ICC/IF, IP, P, WB |
0.1 mg /
€330.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| SM2251PT | FCER1A antibody |
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|
Mouse | IgG2b | 9E1 | Purified | Hu | E, F, ICC/IF, IP, P, WB |
25 µg /
€180.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| SM2251R | FCER1A antibody |
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Mouse | IgG2b | 9E1 | PE | Hu | F |
100 Tests /
€480.00
|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203321 | FCER1A - human gene knockout kit via CRISPR |
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|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203321BN | FCER1A - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203321LP | FCER1A - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203321RB | FCER1A - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400730 | FCER1A overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
