FEM1B antibody
Principal name
FEM1B antibody
Alternative names for FEM1B antibody
F1AA, KIAA0396, FEM1-beta, F1A-alpha, Protein fem-1 homolog B, Fem-1-like death receptor-binding protein alpha
Ncbi ID
9606, NP_056137.1, NP_056137, NM_015322, AAF05314, NP_034323.1, NM_010193, NM_001108157
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of FEM1B antibody
- Kit Component:
- - KN305892G1, Fem1b gRNA vector 1 in pCas-Guide vector
- KN305892G2, Fem1b gRNA vector 2 in pCas-Guide vector
- KN305892D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kamura,T., (2004) Genes Dev. 18 (24), 3055-3065
Western Blot analysis of FEM1B expression in transfected 293T cell line (H00010116-T01) by FEM1B MaxPab polyclonal antibody.Lane 1: FEM1B transfected lysate(68.97 KDa).Lane 2: Non-transfected lysate.
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, Cortex.
Human HEK293T; . Lanes: 1: 30ug HEK293T cell lysate, 2: 30ug 3FLAG-hFEM1B transfected HEK293T cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:8000. Gene Name: FEM1B. Submitted by: Anonymous. FEM1B is strongly supported by BioGPS gene expression data to be expressed in HEK293T.; FEM1B antibody - middle region (ARP53702_P050) in Human HEK293T cells using Western Blot
Human 721_B; WB Suggested Anti-FEM1B Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. FEM1B is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; FEM1B antibody - middle region (ARP53702_P050) in Human 721_B cells using Western Blot
Hum. Fetal Heart; Host:Rabbit. Target Name:FEM1B. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; FEM1B antibody - C-terminal region (ARP53703_P050) in Hum. Fetal Heart cells using Western Blot
Human HEK293T; . Lanes: 1: 30ug HEK293T cell lysate, 2: 30ug 3FLAG-hFEM1B transfected HEK293T cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:8000. Gene Name: FEM1B. Submitted by: Anonymous. FEM1B is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; FEM1B antibody - C-terminal region (ARP53703_P050) in Human HEK293T cells using Western Blot
Human Lung; WB Suggested Anti-FEM1B Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Lung; FEM1B antibody - C-terminal region (ARP53703_P050) in Human Lung cells using Western Blot
Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 ug/ml.
Immunohistochemistry of F1Aa in mouse liver tissue with F1Aa antibody at 5 ug/ml.
Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 ug/mL.
Lanes: 1: 30ug HEK293T cell lysate, 2: 30ug 3FLAG-hFEM1B transfected HEK293T cell lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 8000; Gene Name: FEM1B; Submitted by: Anonymous; FEM1B is strongly supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-FEM1B Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate. FEM1B is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
WB Suggested Anti-FEM1B Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Lung
Host: Rabbit; Target Name: FEM1B; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml
Lanes: 1: 30ug HEK293T cell lysate, 2: 30ug 3FLAG-hFEM1B transfected HEK293T cell lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 8000; Gene Name: FEM1B; Submitted by: Anonymous; FEM1B is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306041 | FEM1B (C-term) antibody |
||||||
| Rabbit Polyclonal F1A alpha Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| AP07752PU-N | FEM1B (609-622) antibody |
||||||
 |
Rabbit | Aff - Purified | Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Rb, Rt | P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303618 | FEM1B |
||||
| Recombinant protein of human fem-1 homolog b (C. elegans) (FEM1B) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414619 | FEM1B overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209569L2V | Lenti ORF particles, Fem1b (GFP-tagged) - Mouse feminization 1 homolog b (C. elegans) (Fem1b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,360.00
|
|
| OriGene Technologies, Inc. | ||
| MR209569L1V | Lenti ORF particles, Fem1b (Myc-DDK-tagged) - Mouse feminization 1 homolog b (C. elegans) (Fem1b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,220.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR206418L2V | Lenti ORF particles, Fem1b (GFP-tagged ORF) - Rat feminization 1 homolog b (C. elegans) (Fem1b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,370.00
|
|
| OriGene Technologies, Inc. | ||
| RR206418L1V | Lenti ORF particles, Fem1b (Myc-DDK-tagged ORF) - Rat feminization 1 homolog b (C. elegans) (Fem1b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,220.00
|
|
| OriGene Technologies, Inc. | ||