Ferrochelatase antibody
Principal name
Ferrochelatase antibody
Alternative names for Ferrochelatase antibody
Protoheme ferro-lyase, Heme synthetase, FECH
Gene ID
2235 (FECH)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of Ferrochelatase antibody
Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria.Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria. Two transcript variants encoding different isoforms have been found for this gene.Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria. Two transcript variants encoding different isoforms have been found for this gene.
General readings
Maruyama,K. (2005) Blood 106 (3), 1098-1104
Western Blot analysis of FECH expression in transfected 293T cell line (H00002235-T01) by FECH MaxPab polyclonal antibody.Lane 1: FECH transfected lysate(46.53 KDa).Lane 2: Non-transfected lysate.
Mouse liver, heart and brain mitochondria; . Lanes: 1. 6 ug mouse liver, heart and brain mitochondria extract 2. 6 ug mouse liver, heart and brain mitochondria extract 3. 6 ug mouse liver, heart and brain mitochondria extract. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:3000. Gene Name: FECH. Submitted by: Dr. Hao Zhu, University of Kansas Medical Center.; FECH antibody - N-terminal region (ARP41682_T100) in Mouse liver, heart and brain mitochondria cells using Western Blot
Human Jurkat; WB Suggested Anti-FECH Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate. FECH is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; FECH antibody - N-terminal region (ARP41682_T100) in Human Jurkat cells using Western Blot
Human Liver; Rabbit Anti-FECH Antibody. Catalog Number: ARP41865. Paraffin Embedded Tissue: Human Liver. Cellular Data: Hepatocytes. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; FECH antibody - N-terminal region (ARP41865_P050) in Human Liver cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-FECH Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. FECH is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; FECH antibody - N-terminal region (ARP41865_P050) in Human Jurkat cells using Western Blot
FECH MaxPab polyclonal antibody. Western Blot analysis of FECH expression in human liver.
Western Blot analysis of FECH expression in transfected 293T cell line (H00002235-T02) by FECH MaxPab polyclonal antibody.Lane 1: FECH transfected lysate(46.53 KDa).Lane 2: Non-transfected lysate.
Mouse liver, heart and brain mitochondria; . Lanes: 1. 6 ug mouse liver, heart and brain mitochondria extract 2. 6 ug mouse liver, heart and brain mitochondria extract 3. 6 ug mouse liver, heart and brain mitochondria extract. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:3000. Gene Name: FECH. Submitted by: Dr. Hao Zhu, University of Kansas Medical Center.; FECH antibody - middle region (ARP41683_P050) in Mouse liver, heart and brain mitochondria cells using Western Blot
Human MCF-7; WB Suggested Anti-FECH Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate. FECH is supported by BioGPS gene expression data to be expressed in MCF7.; FECH antibody - middle region (ARP41683_P050) in Human MCF-7 cells using Western Blot
WB Suggested Anti-FECH Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateFECH is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Rabbit Anti-FECH Antibody; Paraffin Embedded Tissue: Human Liver; Cellular Data: Hepatocytes; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN208058 | FECH - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208058BN | FECH - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208058LP | FECH - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208058RB | FECH - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308058 | Ferrochelatase (transcript variant 2) |
||||
| Recombinant protein of human ferrochelatase (protoporphyria) (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP315223 | Ferrochelatase (transcript variant 1) |
||||
| Purified recombinant protein of Homo sapiens ferrochelatase (protoporphyria) (FECH), nuclear gene encoding mitochondrial protein, transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||