FGF12 antibody
Principal name
FGF12 antibody
Alternative names for FGF12 antibody
Fibroblast growth factor 12, FGF-12, Myocyte-activating factor, FGF12B, FHF1, Fibroblast growth factor homologous factor 1
Ncbi ID
9606, NP_004104.3, NM_021032, NM_004113, NP_066360, NP_004104, NM_010199, NM_183064, NM_130814, NP_034329.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of FGF12 antibody
- Kit Component:
- - KN305923G1, Fgf12 gRNA vector 1 in pCas-Guide vector
- KN305923G2, Fgf12 gRNA vector 2 in pCas-Guide vector
- KN305923D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hubert T., et al. (2008). J Comp Neurol. 507(4):1588-601.
Nakayama F., et al. (2008). J Radiat Res. 49(5):491-501.
Immunoperoxidase of monoclonal antibody to FGF12 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml]
Western Blot analysis of FGF12 expression in transfected 293T cell line by FGF12 monoclonal antibody (M10), clone 1D9.Lane 1: FGF12 transfected lysate(27.4 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged FGF12 is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of FGF12 expression in transfected 293T cell line (H00002257-T01) by FGF12 MaxPab polyclonal antibody.Lane 1: FGF12 transfected lysate(27.40 KDa).Lane 2: Non-transfected lysate.
Recombinant FGF12, His-tagged: 15% SDS-PAGE (3 µg)
Transfected cell immunofluorescence: COS cells expressing GFP-tagged FGF12b/FHF1b. Red = N94/17, Green = GFP, Blue = Hoechst nuclear stain.
Transfected cell immunofluorescence: COS cells expressing GFP-tagged FGF12b/FHF1b. Red = N94/17, Green = GFP, Blue = Hoechst nuclear stain.
Predicted band size: 20 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 50 seconds)
Predicted band size: 20 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 50 seconds)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA322793 | FGF12 antibody |
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| Anti-FGF12 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322794 | FGF12 antibody |
||||||
| Anti-FGF12 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| 73-135 | FGF12 antibody |
||||||
 |
Mouse | IgG1 | N94/17 | Supernatant | Hu, Ms, Rt | ICC/IF |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
|
|||||||
| 75-135 | FGF12 antibody |
||||||
 |
Mouse | IgG1 | N94/17 | Purified | Hu, Ms, Rt | ICC/IF |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
|
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP315868 | FGF12 (transcript variant 1) |
||||
| Recombinant protein of human fibroblast growth factor 12 (FGF12), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720197 | FGF12 (transcript variant 2) |
||||
| Recombinant protein of human fibroblast growth factor 12 (FGF12), transcript variant 2 | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412153 | FGF12 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY418206 | FGF12 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC215868L2V | Lenti ORF particles, FGF12 (mGFP-tagged) - Human fibroblast growth factor 12 (FGF12), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201628L2V | Lenti ORF particles, Fgf12 (GFP-tagged) - Mouse fibroblast growth factor 12 (Fgf12), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201628L1V | Lenti ORF particles, Fgf12 (Myc-DDK-tagged) - Mouse fibroblast growth factor 12 (Fgf12), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213375L2V | Lenti ORF particles, Fgf12 (GFP-tagged ORF) - Rat fibroblast growth factor 12 (Fgf12), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR213375L1V | Lenti ORF particles, Fgf12 (Myc-DDK-tagged ORF) - Rat fibroblast growth factor 12 (Fgf12), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
