FGF4 antibody
Principal name
FGF4 antibody
Alternative names for FGF4 antibody
Fibroblast growth factor 4, HST-1, HST, HSTF1, KS3, Heparin secretory-transforming protein, Transforming protein KS3, Heparin-binding growth factor 4
Ncbi ID
NP_001998, 9606, NP_001998.1, AAI56334.1, NM_002007, P08620, NP_034332.2, NM_010202
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of FGF4 antibody
Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with FGF4 polyclonal antibody ( Cat # PAB4025 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The FGF4 polyclonal antibody ( Cat # PAB4025 ) is used in Western blot to detect FGF4 in Jurkat cell lysate.
Western blot analysis of FGF4 in NIH NIH/3T3 cell lysate with FGF4 polyclonal antibody ( Cat # PAB13022 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
Immunohistochemical analysis of paraffin-embedded human lung cancer sections using FGF4 polyclonal antibody ( Cat # PAB7973 ) .
Western blot analysis of HeLa cell lysate. Using FGF4 polyclonal antibody ( Cat # PAB7973 ) .
Immnohistochemical analysis of paraffin embedded Human lung cancer sections using FGF4 antibody
Western blot analysis og Hela cell lysis using FGF4 antibody
Western blot analysis of FGF4 in NIH 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry of FGF4 in 3T3 cells with FGF4 antibody at 2.5 ug/mL.
Immunofluorescence of FGF4 in 3T3 cells with FGF4 antibody at 2.5 ug/mL.
Predicted cell location: Cytoplasm. Positive control: Human liver and thyroid cancer tissue. Recommended dilution: 1/50-150 The image on the left is immunohistochemistry of paraffin-embedded Human liver and thyroid cancer tissue using FGF4 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human liver and thyroid cancer tissue. Recommended dilution: 1/50-150 The image on the left is immunohistochemistry of paraffin-embedded Human liver and thyroid cancer tissue using FGF4 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of anti-FGF4 Antibody (C-term) (Cat.#TA325033) in CEM cell line lysates (35ug/lane). FGF4 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with FGF4 Antibody (C-term) (Cat.#TA325033), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
This antibody stained formalin-fixed paraffin-embedded sections of normal breast tissue from a breast carcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306516 | FGF4 antibody |
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| Rabbit Polyclonal FGF4 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| TA323076 | FGF4 antibody |
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| Anti-FGF4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA325033 | FGF4 (C-term) antibody |
||||||
| Rabbit polyclonal FGF4 Antibody (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA328312 | FGF4 antibody |
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| Anti-Human FGF-4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 mg /
€275.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP01123BT-N | FGF4 antibody |
||||||
 |
Rabbit | Biotin | Hu | E, WB |
50 µg /
€340.00
|
||
| OriGene Technologies GmbH | |||||||
| AP01123BT-S | FGF4 antibody |
||||||
|
Rabbit | Biotin | Hu | E, WB |
25 µg /
€250.00
|
||
| OriGene Technologies GmbH | |||||||
| AP01123PU-N | FGF4 antibody |
||||||
|
Rabbit | Aff - Purified | Hu | E, WB |
0.1 mg /
€340.00
|
||
| OriGene Technologies GmbH | |||||||
| AP01123PU-S | FGF4 antibody |
||||||
|
Rabbit | Aff - Purified | Hu | E, WB |
50 µg /
€250.00
|
||
| OriGene Technologies GmbH | |||||||
| AP23262PU-N | FGF4 (C-term) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€390.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP723098 | FGF4 |
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| Purified recombinant protein of Human fibroblast growth factor 4 (FGF4). | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
25 µg /
€230.00
|
|
| OriGene Technologies, Inc. | |||||
| TP723876 | FGF4 |
||||
| Purified recombinant protein of Human fibroblast growth factor 4 (FGF4) | |||||
|
Human | > 95 % Preparation: . Purity Detail: >95%, as determined by Coomassie stained SDS-PAGE. |
E. coli |
10 µg /
€175.00
|
|
| OriGene Technologies, Inc. | |||||
| TP750009 | FGF4 |
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| Recombinant protein of human Fibroblast Growth Factor-4 (FGF4) produced in E. coli. | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
0.1 mg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419590 | FGF4 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
