FHIT antibody
Principal name
FHIT antibody
Alternative names for FHIT antibody
Dinucleosidetriphosphatase, AP3Aase, Bis(5'-adenosyl)-triphosphatase, AP3A hydrolase, Fragile histidine triad protein
Ncbi ID
9606, NP_001159715.1, NP_002003, NM_002012, NP_001159715, NP_034340.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of FHIT antibody
- Kit Component:
- - KN305959G1, Fhit gRNA vector 1 in pCas-Guide vector
- KN305959G2, Fhit gRNA vector 2 in pCas-Guide vector
- KN305959D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kmiec Z., et al. (2009) J Physiol Pharmacol. 60(4):63-70.
Ahmad F., et al. (2010) J Cancer Res Clin Oncol. 136(3):333-50.
Detection limit for recombinant GST tagged FHIT is approximately 0.1ng/ml as a capture antibody.
FHIT monoclonal antibody (M01), clone 1E3 Western Blot analysis of FHIT expression in HL-60 ( Cat # L014V1 ).
Detection limit for recombinant GST tagged FHIT is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged FHIT is approximately 10ng/ml as a capture antibody.
Detection limit for recombinant GST tagged FHIT is approximately 10ng/ml as a capture antibody.
Detection limit for recombinant GST tagged FHIT is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged FHIT is approximately 1ng/ml as a capture antibody.
Immunohistochemistry (IHC) analyzes of FHIT antibody (Cat.-No.: AP06121PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of FHIT antibody (Cat.-No.: AP06121PU-N) in extracts from A549 cells.
Human breast stained with anti-FHIT antibody
Immunohistochemical staining of FHIT on formalin fixed, paraffin embedded human breast with FHIT polyclonal antibody.
Recombinant human FHIT, 1-147 aa, His-tagged: 15% SDS-PAGE (3 ug)
Human kidney; WB Suggested Anti-FHIT Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human kidney; FHIT antibody - middle region (ARP51169_P050) in Human kidney cells using Western Blot
Immunohistochemical analysis of FHIT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of FHIT staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of FHIT expression in HEK293T (A) whole cell lysates.
Western blot analysis of extracts from A549 cells, using FHIT antibody (#TA313873).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FHIT antibody (#TA313873).The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using FHIT antibody (#TA313873).The picture on the right is treated with the synthesized peptide.
Predicted band size: 17 kDa . Positive control: Mouse brain and human fetal kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%+12%SDS-PAGE Lane1: Mouse brain tissue lysate Lane2: Human fetal kidney tissue lysate Lysates: 30 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using FHIT antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted band size: 17 kDa . Positive control: Human fetal kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%+12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using FHIT antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200)
Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 ug/ml.
Immunofluorescence of FHIT in HeLa cells with FHIT antibody at 5 ug/mL.
Western blot analysis of K562 cell lysate using FHIT antibody.
WB Suggested Anti-FHIT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human kidney
Western blot analysis on A549 cell lysate using FHIT Antibody
Human Breast stained with anti-Fragile Histidine Triad (FHIT) antibody.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse kidney tissue, human kidney tissue, Primary antibody: (FHIT Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (FHIT Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (FHIT Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313873 | FHIT antibody |
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| Rabbit polyclonal anti-FHIT antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA323982 | FHIT antibody |
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| Anti-FHIT Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA323983 | FHIT antibody |
||||||
| Anti-FHIT Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA326647 | FHIT antibody |
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| Rabbit Polyclonal FHIT Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA326988 | FHIT antibody |
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| FHIT Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347696 | FHIT antibody |
||||||
| Rabbit Polyclonal Anti-FHIT Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349983 | FHIT antibody |
||||||
| Rabbit Polyclonal Anti-FHIT Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP06121PU-N | FHIT antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307120 | FHIT |
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| Recombinant protein of human fragile histidine triad gene (FHIT) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328740 | FHIT (transcript variant 2) |
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| Recombinant protein of human fragile histidine triad gene (FHIT), transcript variant 2. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||