FHL1 antibody
Principal name
FHL1 antibody
Alternative names for FHL1 antibody
SLIM1, FHL1, FHL-1, SLIM-1, SLIM, Four and a half LIM domains protein 1, Skeletal Muscle LIM-protein 1
Ncbi ID
9606, NP_001153171.1, NP_034341, NP_001440, NM_001449, NM_001159703, NM_001159701, NM_001159702, NM_001159704, NP_001153175, NP_001153173, NP_001153172, NP_001153176, NP_001070830.1, NM_001077362, NM_010211, NM_001077361, NM_001159700, NM_001159699
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of FHL1 antibody
- Kit Component:
- - KN305960G1, Fhl1 gRNA vector 1 in pCas-Guide vector
- KN305960G2, Fhl1 gRNA vector 2 in pCas-Guide vector
- KN305960D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication. J Biol Chem. 1999 Sep 17;274(38):27083-91.
Western Blot analysis of FHL1 expression in transfected 293T cell line by FHL1 monoclonal antibody (M01), clone 2A9.Lane 1: FHL1 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
FHL1 monoclonal antibody (M01), clone 2A9. Western Blot analysis of FHL1 expression in PC-12(Cat # L012V1 ).
FHL1 monoclonal antibody (M01), clone 2A9. Western Blot analysis of FHL1 expression in NIH/3T3(Cat # L018V1 ).
FHL1 monoclonal antibody (M01), clone 2A9. Western Blot analysis of FHL1 expression in Raw 264.7.
Detection limit for recombinant GST tagged FHL1 is approximately 0.3ng/ml as a capture antibody.
Human kidney; FHL1 antibody - C-terminal region (ARP34378_T100) in Human kidney cells using Immunohistochemistry
Human Fetal Heart; Host: Rabbit. Target Name:FHL1. Sample Tissue:Human Fetal Heart. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 0.12.; FHL1 antibody - C-terminal region (ARP34378_T100) in Human Fetal Heart cells using Western Blot
Human Muscle; WB Suggested Anti-FHL1 Antibody Titration: 0.1ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; FHL1 antibody - C-terminal region (ARP34378_T100) in Human Muscle cells using Western Blot
FHL1 polyclonal antibody ( Cat # PAB6686 ) staining ( 0.05 µg/ml ) of human muscle lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
FHL1 polyclonal antibody ( Cat # PAB6687 ) ( 0.01 µg/ml ) staining of human heart lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
FHL1 monoclonal antibody (M05), clone 2F7. Western Blot analysis of FHL1 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged FHL1 is approximately 0.3ng/ml as a capture antibody.
FHL1 MaxPab rabbit polyclonal antibody. Western Blot analysis of FHL1 expression in human kidney.
Western Blot analysis of FHL1 expression in transfected 293T cell line (H00002273-T01) by FHL1 MaxPab polyclonal antibody.Lane 1: FHL1 transfected lysate(31.90 KDa).Lane 2: Non-transfected lysate.
Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
Antibody staining (0.05 µg/ml) of Human Muscle lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Hum. Fetal Brain; Host: Rabbit . Target Name: FHL1 . Sample Tissue: Human Fetal Brain . Antibody Dilution: 1.0ug/ml.; FHL1 antibody - N-terminal region (ARP31902_P050) in Hum. Fetal Brain cells using Western Blot
Hum. Fetal Brain; Host: Rabbit . Target Name: FHL1 . Sample Tissue: Human Fetal Brain . Antibody Dilution: 1.0ug/ml.; FHL1 antibody - N-terminal region (ARP31902_P050) in Hum. Fetal Brain cells using Western Blot
Human Brain; WB Suggested Anti-FHL1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; FHL1 antibody - N-terminal region (ARP31902_P050) in Human Brain cells using Western Blot
Western blot analysis of FHL1 Antibody (C-term) (AP17369PU-N) in NIH-3T3 cell line lysates (35 µg/lane). FHL1 (arrow) was detected using the purified Pab.
Anti-FHL1 mouse monoclonal antibody (TA501279) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody(TA501279), and then analyzed by flow cytometry.
Anti-FHL1 mouse monoclonal antibody (TA501280) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.
HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody(TA501280), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.
HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody(TA501295), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-FHL1 antibody(TA501295),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-FHL1 mouse monoclonal antibody (TA501296) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-FHL1 antibody(TA501296),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-FHL1 antibody(TA501296),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-FHL1 mouse monoclonal antibody (TA501375) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody(TA501375), and then analyzed by flow cytometry.
TA302486 staining (0.05µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302612 (0.01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-FHL1 Antibody Titration: 0.1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle
Host: Rabbit; Target Name:FHL1; Sample Tissue:Human Fetal Heart; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:1 ug/ml; Peptide Concentration: 5ug/ml; Lysate Quantity: 25ug/lane/lane; Gel Concentration: 0.12
Human kidney
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Human adrenal pheochromocytoma tissue, Human fetal muscle tissue, Human skeletal muscle tissue, Primary antibody: (FHL1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (FHL1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (FHL1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
WB Suggested Anti-FHL1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain
Host: Rabbit; Target Name: FHL1; Sample Tissue: Human Fetal Brain; Antibody Dilution: 1.0ug/ml
Host: Rabbit; Target Name: FHL1; Sample Tissue: Human Fetal Brain; Antibody Dilution: 1.0ug/ml
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA501279 | FHL1 antibody |
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| FHL1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11) | |||||||
 |
Mouse | IgG1 | OTI2E11 | Purified | Can, Hu, Rt | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA501295 | FHL1 antibody |
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| anti-FHL1 mouse monoclonal antibody, clone OTI1D6 (formerly 1D6) | |||||||
 |
Mouse | IgG1 | OTI1D6 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA501296 | FHL1 antibody |
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| FHL1 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7) | |||||||
 |
Mouse | IgG2b | OTI3G7 | Purified | Hu, Rt | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA501375 | FHL1 antibody |
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| anti-FHL1 mouse monoclonal antibody, clone OTI3H3 (formerly 3H3) | |||||||
 |
Mouse | IgG1 | OTI3H3 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA302486 | FHL1 antibody |
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| Goat Polyclonal Antibody against FHL1 / SLIM1 | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA302612 | FHL1 antibody |
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| Goat Polyclonal Antibody against SLIMMER / FHL1 isoform B | |||||||
 |
Goat | Aff - Purified | Hu, Por | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA349984 | FHL1 antibody |
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| Rabbit Polyclonal Anti-FHL1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA501280 | FHL1 antibody |
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| anti-FHL1 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1) | |||||||
 |
Mouse | IgG1 | OTI2E1 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP08838PU-N | FHL1 antibody |
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 |
Goat | IgG | Aff - Purified | Hst, Hu, Ms, Rt | E, P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP17369PU-N | FHL1 (C-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203478 | FHL1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203478BN | FHL1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203478LP | FHL1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203478RB | FHL1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303478 | FHL1 |
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| Recombinant protein of human four and a half LIM domains 1 (FHL1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328125 | FHL1 (transcript variant 6) |
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| Purified recombinant protein of Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 6. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328243 | FHL1 (transcript variant 5) |
||||
| Purified recombinant protein of Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 5. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328799 | FHL1 (transcript variant 3) |
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| Recombinant protein of human four and a half LIM domains 1 (FHL1), transcript variant 3. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328800 | FHL1 (transcript variant 4) |
||||
| Recombinant protein of human four and a half LIM domains 1 (FHL1), transcript variant 4. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400563 | FHL1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
