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FHL5 antibody

Principal name

FHL5 antibody

Alternative names for FHL5 antibody

FHL5, ACT, Four and a half LIM domains protein 5, FHL-5, Activator of CREM in testis, Activator of cAMP-responsive element modulator in testis

SwissProt ID

Q2YDK0 (Bovin), Q4R7A4 (Macfa), Q5TD97 (Human), Q6AXT1 (Rat), Q9WTX7 (Mouse)

Gene ID

9457 (FHL5), 57756 (Fhl5), 297954

Ncbi ID

NP_001164278.1, NP_065228, NM_020482, NM_001170807, NP_067293.1, NM_021318, NM_001013088

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of FHL5 antibody

FHL5 is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa.The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Belbin,O., (er) Neurobiol. Aging (2007) In press

Immunofluorescence of monoclonal antibody to FHL5 on HepG2 cell. [antibody concentration 10 ug/ml]

H00009457-M01

Western Blot analysis of FHL5 expression in transfected 293T cell line by FHL5 monoclonal antibody (M01), clone 1G12-D2.Lane 1: FHL5 transfected lysate(32.8 KDa).Lane 2: Non-transfected lysate.

H00009457-M01

Western Blot analysis of FHL5 expression in transfected 293T cell line (H00009457-T01) by FHL5 MaxPab polyclonal antibody.Lane 1: FHL5 transfected lysate(31.24 KDa).Lane 2: Non-transfected lysate.

H00009457-B01

Western Blot analysis of FHL5 expression in transfected 293T cell line (H00009457-T02) by FHL5 MaxPab polyclonal antibody.Lane 1: FHL5 transfected lysate(32.80 KDa).Lane 2: Non-transfected lysate.

H00009457-D01P

Human Placenta; WB Suggested Anti-FHL5 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta; FHL5 antibody - middle region (ARP51673_P050) in Human Placenta cells using Western Blot

ARP51673_P050

Western Blot analysis of FHL5 expression in transfected 293T cell line (H00009457-T02) by FHL5 MaxPab polyclonal antibody.Lane 1: FHL5 transfected lysate(32.80 KDa).Lane 2: Non-transfected lysate.

H00009457-D01

Immunoprecipitation of FHL5 transfected lysate using anti-FHL5 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with FHL5 MaxPab mouse polyclonal antibody (B01) (H00009457-B01).

H00009457-D01

Sample (30 ug of whole cell lysate). A: 293T. B: Raji. 12 % SDS PAGE. TA308694 diluted at 1:1000

TA308694

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using FHL5(TA308694) antibody at 1:500 dilution.

TA308694

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5(TA308694) antibody at 1:500 dilution.

TA308694

Predicted cell location: Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using FHL5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200)

TA324026

Predicted cell location: Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using FHL5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)

TA324026

WB Suggested Anti-FHL5 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta

TA339239

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA308694	FHL5 antibody
	Rabbit Polyclonal antibody to FHL5 (four and a half LIM domains 5)
	Rabbit	IgG	Aff - Purified	Hu	ICC/IF, P, WB	0.1 ml / €415.00
						OriGene Technologies, Inc.
+2 additional images
TA324026	FHL5 antibody
	Anti-FHL5 Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image

Lysates

Catalog No.
LY412449	FHL5 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY432828	FHL5 overexpression lysate
		0.1 mg / €315.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC229828L1V	Lenti ORF particles, FHL5 (Myc-DDK tagged) - Human four and a half LIM domains 5 (FHL5), transcript variant 2 , 200 uL, >10^7 TU/mL
		200 µl / €910.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR203852L2V	Lenti ORF particles, Fhl5 (GFP-tagged) - Mouse four and a half LIM domains 5 (Fhl5), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR203852L1V	Lenti ORF particles, Fhl5 (Myc-DDK-tagged) - Mouse four and a half LIM domains 5 (Fhl5), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR201649L2V	Lenti ORF particles, Fhl5 (GFP-tagged ORF) - Rat four and a half LIM domains 5 (Fhl5), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR201649L1V	Lenti ORF particles, Fhl5 (Myc-DDK-tagged ORF) - Rat four and a half LIM domains 5 (Fhl5), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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