Home » Target » Fibrillin-1 antibody

Fibrillin-1 antibody

Principal name

Fibrillin-1 antibody

Alternative names for Fibrillin-1 antibody

FBN1, FBN

SwissProt ID

P35555 (Human), P98133 (Bovin), Q61554 (Mouse), Q9TV36 (Pig)

Gene ID

2200 (FBN1)

Ncbi ID

9606, BAD16737, NP_000129.3, NP_000129, NM_000138

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoelectron Microscopy (EM)

Background of Fibrillin-1 antibody

The fibrillin family of proteins, including fibrillin-1 (FBN1) and fibrillin-2 (FBN2), are integral components of a distinct subset of extracellular microfibrils. Microfibrils are found in elastic tissues where they facilitate elastic fiber formation and in nonelastic tissue where they support the association of the epithelial cells with the interstitial matrix. Characteristic of the fibrillin proteins are the epidermal growth factor (EGF)-like motifs, which contain a consensus sequence for calcium binding. This calcium association may be critical for protein-protein interactions and stabilization of the microfibrils. Mutations of the FBN1 gene have been shown to result in Marfan syndrome, a disease characterized by abnormal synthesis, secretion and matrix deposition of fibrillin. FBN2 is also linked to a rare, yet similiar, skeletal disorder, congenital contractural arachnodactyly.

General readings

Uyeda,T., et al., (2004) J. Hum. Genet. 49 (8), 404-407

Immunoperoxidase of monoclonal antibody to FBN1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]

H00002200-M01

Human Jurkat; WB Suggested Anti-FBN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; FBN1 antibody - N-terminal region (ARP37969_P050) in Human Jurkat cells using Western Blot

ARP37969_P050

Immunohistochemistry analysis of Fibrillin-1 Antibody (Cat.-No AP06122PU-N) in paraffin-embedded human breast carcinoma tissue.

AP06122PU-N

Western blot (WB) analysis of FBN1 (N2843) pAb at 1:500 dilution: Lane 1: A549 whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: PC12 whole cell lysate.

AP06122PU-N

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Fibrillin-1 Antibody. The picture on the right is treated with the synthesized peptide.

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM31783PU-N

Detection limit for recombinant GST tagged FBN1 is 0.3 ng/ml as a capture antibody.

AM31783PU-N

Western blot analysis of Fibrillin 1 expression in Jurkat (A); NIH3T3 (B) whole cell lysates.

Immunofluorescent analysis of Fibrillin 1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Fibrillin-1 antibody.The picture on the right is treated with the synthesized peptide.

TA311656

Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using FBN1 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)

TA323728

Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using FBN1 antibody at dilution 1/47, on the right is treated with the synthetic peptide. (Original magnification:x200)

TA323729

WB Suggested Anti-FBN1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate

TA335164

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA311656	Fibrillin-1 antibody
	Rabbit polyclonal Fibrillin-1 antibody
	Rabbit	IgG		Purified	Hu, Ms	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA323728	Fibrillin-1 antibody
	Anti-FBN1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA323729	Fibrillin-1 antibody
	Anti-FBN1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
AM08022BT-N	Fibrillin-1 antibody
	Mouse	IgG1	11C1.3	Biotin	Bov, Hu	C, EM, ICC/IF, WB	0.2 mg / €550.00
							OriGene Technologies GmbH
AM08022FC-N	Fibrillin-1 antibody
	Mouse	IgG1	11C1.3	FITC	Bov, Hu	C, ICC/IF	0.2 mg / €530.00
							OriGene Technologies GmbH
AM08022PU-N	Fibrillin-1 antibody
	Mouse	IgG1	11C1.3	Purified	Bov, Hu	C, EM, ICC/IF, WB	0.2 mg / €410.00
							OriGene Technologies GmbH
AM31783PU-N	Fibrillin-1 (2772-2872) antibody
	Mouse	IgG2a	3H6	Purified	Hu	E, P, WB	50 µg / €475.00
							OriGene Technologies GmbH
+1 additional image
AP06122PU-N	Fibrillin-1 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.
LY424903	FBN1 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Antibodies (see all»)

Kits (see all»)

Antigens

Lysates (see all»)

Tissue Slides (see all»)

Other life-science products