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FKBP4 antibody

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Principal name

FKBP4 antibody

Alternative names for FKBP4 antibody

FK506-binding protein 4, FKBP52 protein, FKBP59, p59 protein, Peptidyl-prolyl cis-trans isomerase, PPIase, HSP-binding immunophilin, HBI, 52 kDa FK506-binding protein, Rotamase

SwissProt ID

P27124 (Rabit), P30416 (Mouse), Q02790 (Human), Q9QVC8 (Rat), Q9TRY0 (Bovin)

Gene ID

2288 (FKBP4), 14228 (Fkbp4)

Ncbi ID

9606, NP_002005.1, NP_002005, 10090, NM_002014, NM_010219

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Hst (Hamster), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), EPair for Elisa (EPair), Flow Cytometry (F)

Background of FKBP4 antibody

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin Abinding families of immunophilins (2).
FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3).
The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4).
Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5).

General readings

Ruan B, Pong K, Jow F, Bowlby M, Crozier RA, Liu D, Liang S, Chen Y, Mercado ML, Feng X, Bennett F, von Schack D, McDonald L, Zaleska MM, Wood A, Reinhart PH, Magolda RL, Skotnicki J, Pangalos MN, Koehn FE, Carter GT, Abou-Gharbia M, Graziani EI.
Binding of rapamycin analogs to calcium channels and FKBP52 contributes to their neuroprotective activities.
Proc. Natl. Acad. Sci. U.S.A. 2008 Jan 105 (1): 33-8.
PMID: 18162540

H00002288-M01 - FKBP4
Immunoperoxidase of monoclonal antibody to FKBP4 on formalin-fixed paraffin-embedded human uterine cervix. [antibody concentration 0.5 ug/ml]
H00002288-M01 - FKBP4
FKBP4 monoclonal antibody (M01), clone 5C11. Western Blot analysis of FKBP4 expression in NIH/3T3 ( Cat # L018V1 ).
H00002288-M01 - FKBP4
FKBP4 monoclonal antibody (M01), clone 5C11. Western Blot analysis of FKBP4 expression in Raw 264.7 ( Cat # L024V1 ).
H00002288-M01 - FKBP4
FKBP4 monoclonal antibody (M01), clone 5C11 Western Blot analysis of FKBP4 expression in HeLa ( Cat # L013V1 ).
H00002288-M01 - FKBP4
FKBP4 monoclonal antibody (M01), clone 5C11. Western Blot analysis of FKBP4 expression in PC-12 ( Cat # L012V1 ).
H00002288-M01 - FKBP4
Detection limit for recombinant GST tagged FKBP4 is approximately 1ng/ml as a capture antibody.
H00002288-B01 - FKBP4
Immunoperoxidase of purified MaxPab antibody to FKBP4 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml]
H00002288-B01 - FKBP4
Immunofluorescence of purified MaxPab antibody to FKBP4 on HeLa cell. [antibody concentration 10 ug/ml]
H00002288-B01 - FKBP4
FKBP4 MaxPab polyclonal antibody. Western Blot analysis of FKBP4 expression in human liver.
H00002288-B01 - FKBP4
Western Blot analysis of FKBP4 expression in transfected 293T cell line (H00002288-T01) by FKBP4 MaxPab polyclonal antibody.Lane 1: FKBP4 transfected lysate(50.49 KDa).Lane 2: Non-transfected lysate.
H00002288-B01 - FKBP4
FKBP4 MaxPab polyclonal antibody. Western Blot analysis of FKBP4 expression in HeLa.
AM03154PU-S - FKBP4
Prostate tissue was histologically stained with antibodies specific for FKBP52. The strongest staining for FKBP52 is in ductal epithelial cells. Courtesy of David F. Smith, Mayo Clinic, USA.
H00002288-B01P - FKBP4
Immunoperoxidase of purified MaxPab antibody to FKBP4 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml]
H00002288-B01P - FKBP4
Immunofluorescence of purified MaxPab antibody to FKBP4 on HeLa cell. [antibody concentration 10 ug/ml]
H00002288-B01P - FKBP4
FKBP4 MaxPab polyclonal antibody. Western Blot analysis of FKBP4 expression in human liver.
H00002288-B01P - FKBP4
Western Blot analysis of FKBP4 expression in transfected 293T cell line (H00002288-T01) by FKBP4 MaxPab polyclonal antibody.Lane 1: FKBP4 transfected lysate(50.49 KDa).Lane 2: Non-transfected lysate.
H00002288-B01P - FKBP4
FKBP4 MaxPab polyclonal antibody. Western Blot analysis of FKBP4 expression in HeLa.
H00002288-D01P - FKBP4
FKBP4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in mouse spleen.
H00002288-D01P - FKBP4
FKBP4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in mouse testis.
H00002288-D01P - FKBP4
FKBP4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in human pancreas.
H00002288-D01P - FKBP4
FKBP4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in human liver.
H00002288-D01P - FKBP4
FKBP4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in K-562.
H00002288-D01P - FKBP4
Western Blot analysis of FKBP4 expression in transfected 293T cell line (H00002288-T01) by FKBP4 MaxPab polyclonal antibody.Lane 1: FKBP4 transfected lysate(51.80 KDa).Lane 2: Non-transfected lysate.
H00002288-D01P - FKBP4
FKBP4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in Jurkat.
AR09172PU-L - FKBP4
FKBP4, 1-459aa human, His-tagged, recombinant, E.coli: 15% SDS-PAGE (3 µg)
AM09071PU-N - FKBP4
Immunohistochemistry: Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and Diaminobenzidine (DAB) was used for detection.
AM09071PU-N - FKBP4
Western blot analysis: Cell lysates of HeLa (40 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FKBP4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
PAB11467 - FKBP4
FKBP4 polyclonal antibody ( Cat # PAB11467 ) ( 0.01 µg/ml ) staining of human placenta lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
PAB8722 - FKBP4
Western blot analysis of Fkbp4 in NIH/3T3 cell lysate ( Lane 1 ) and rat kidney tissue lysate ( Lane 2 ) .
MAB3587 - FKBP4
Cell lysates of HeLa ( 40 µg ) were resolved by SDS-PAGE, transferred to NC membrane and probed with FKBP4 monoclonal antibody, clone AT4D3 ( 1 : 1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
AP17384PU-N - FKBP4
Western blot analysis of FKBP4 (arrow) using FKBP4 Antibody (Center) Cat.-No AP17384PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FKBP4 gene.
AP17384PU-N - FKBP4
Western blot analysis of FKBP4 Antibody (Center) Cat.-No AP17384PU-N in Hela, MCF7, T47D cell line lysates(35 µg/lane). FKBP4(arrow) was detected using the purified Pab.
AP17384PU-N - FKBP4
Flow cytometric analysis of MCF-7 cells using FKBP4 Antibody (Center) Cat.-No AP17384PU-N (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ARP30180_P050 - FKBP4
Mouse Brain; . Sample Type: Mouse Brain Slices . Red: primary. Blue: DAPI. Primary. Dilution:.  1:400 . Secondary Antibody: Anti-Rabbit IgG Alexa 594. Secondary. Dilution:.  1:400 . Image Submitted By: Adahir Labrador-Garrido and Cintia Roodveldt . University of Seville. .; FKBP4 antibody - C-terminal region (ARP30180_P050) in Mouse Brain cells using Immunohistochemistry
ARP30180_P050 - FKBP4
Hum. Fetal Lung; Host:Rabbit. Target Name:FKBP4. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; FKBP4 antibody - C-terminal region (ARP30180_P050) in Hum. Fetal Lung cells using Western Blot
ARP30180_P050 - FKBP4
Human 721_B; WB Suggested Anti-FKBP4 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. FKBP4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; FKBP4 antibody - C-terminal region (ARP30180_P050) in Human 721_B cells using Western Blot
TP300713 - FKBP4
TA305784 - FKBP4
TA305784 (0.01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA308638 - FKBP4
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 ug Rat2 whole cell lysate/extract. 10 % SDS-PAGE. FKBP4 antibody (TA308638) dilution: 1:1000
TA308638 - FKBP4
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug A375 whole cell ly
TA308638 - FKBP4
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 ug GL261 whole cell lysate/extract. B. 30 ug C8D30 whole cell lysate/extract. 10 % SDS-PAGE. FKBP4 antibody (TA308638) dilution: 1:1000
TA308638 - FKBP4
Immunofluorescence analysis of paraformaldehyde-fixed A431, using FKBP52(TA308638) antibody at 1:200 dilution.
TA318989 - FKBP4
Western blot analysis of FKBP52 in 3T3 cell lysate.
TA332809 - FKBP4
Western blot analysis of extracts of various cell lines, using FKBP4 antibody.
TA332809 - FKBP4
Immunofluorescence analysis of U20S cell using FKBP4 antibody. Blue: DAPI for nuclear staining.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FKBP4 antibody
Immunohistochemistry: Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and Diaminobenzidine (DAB) was used for detection. Mouse IgG2b AT4D3 Purified Hu E, P, WB
0.1 ml / €330.00
  OriGene Technologies GmbH
+1 additional image

Published recently!

FKBP4 antibody
Immunohistochemistry: Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and Diaminobenzidine (DAB) was used for detection. Mouse IgG2b AT4D3 Purified Hu E, P, WB
50 µl / €220.00
  OriGene Technologies GmbH
+1 additional image

Published recently!

FKBP4 antibody
  Rabbit Polyclonal antibody to FKBP52 (FK506 binding protein 4, 59kDa)  
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 ug Rat2 whole cell lysate/extract. 10 % SDS-PAGE. FKBP4 antibody (TA308638) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

FKBP4 (C-term) antibody
  Goat Anti-FKBP4 / FKBP52 Antibody  
TA305784 (0.01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

FKBP4 antibody
  Mouse monoclonal FKBP52 Antibody  
Prostate tissue was histologically stained with antibodies specific for FKBP52. The strongest staining for FKBP52 is in ductal epithelial cells. Courtesy of David F. Smith, Mayo Clinic, USA. Mouse IgG1 Hi52C Purified Can, Hst, Hu, Ms, Rt IP, P, WB
0.1 mg / €430.00
  OriGene Technologies, Inc.

FKBP4 antibody
  Rabbit anti-FKBP4 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using FKBP4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

FKBP4 antibody
Western blot analysis of FKBP4 (arrow) using FKBP4 Antibody (Center) Cat.-No AP17384PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FKBP4 gene. Rabbit Ig Purified Hu F, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+2 additional images

CRISPR

Catalog No. Species Donor Vector  

FKBP4 - human gene knockout kit via CRISPR
FKBP4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FKBP4 - human gene knockout kit via CRISPR
FKBP4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

FKBP4 - human gene knockout kit via CRISPR
FKBP4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FKBP4 - human gene knockout kit via CRISPR
FKBP4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FKBP4
  Recombinant protein of human FK506 binding protein 4, 59kDa (FKBP4)  
FKBP4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

FKBP4 overexpression lysate
FKBP4 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

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  3. Enzyme Immunoassay (E) (2)
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