FOXG1 antibody
Principal name
FOXG1 antibody
Alternative names for FOXG1 antibody
Forkhead box protein G1, FOXG1A, FOXG1B, FOXG1C, Forkhead-related protein FKHL, FKHL1, FKHL2, FKHL3, FKHL4, HFK1, HFK2, HFK3, Brain factor 1, Brain factor 2
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of FOXG1 antibody
- Kit Component:
- - KN306080G1, Foxg1 gRNA vector 1 in pCas-Guide vector
- KN306080G2, Foxg1 gRNA vector 2 in pCas-Guide vector
- KN306080D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Mencarelli, M.A., et al. J. Med. Genet. 47(1):49-53(2010)
Philippe, C., et al. J. Med. Genet. 47(1):59-65(2010)
Ariani, F., et al. Am. J. Hum. Genet. 83(1):89-93(2008)
Adesina, A.M., et al. J. Neurooncol. 85(2):111-122(2007)
Bredenkamp, N., et al. Dev. Genes Evol. 217(3):227-233(2007)
Human kidney; FOXG1A antibody - N-terminal region (P100984_T100) in Human kidney cells using Immunohistochemistry
Human Liver; FOXG1A antibody - N-terminal region (P100984_T100) in Human Liver cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-FOXG1A Antibody Titration: 5.0-8.0ug/ml. Positive Control: Jurkat cell lysate; FOXG1A antibody - N-terminal region (P100984_T100) in Human Jurkat cells using Western Blot
Human kidney; FOXG1B antibody - N-terminal region (ARP38595_T100) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-FOXG1B Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; FOXG1B antibody - N-terminal region (ARP38595_T100) in Human HepG2 cells using Western Blot
Transfected 293T; WB Suggested Anti-FOXG1B Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; FOXG1B antibody - C-terminal region (ARP38596_T100) in Transfected 293T cells using Western Blot
FOXG1 polyclonal antibody ( Cat # PAB6235 ) staining ( 0.3 µg/ml ) of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Transfected 293T; WB Suggested Anti-FOXG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; FOXG1 antibody - N-terminal region (ARP31686_P050) in Transfected 293T cells using Western Blot
TA302825 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of FOXG1 Antibody (Center) (Cat. #TA325116) in mouse brain tissue lysates (35ug/lane). FOXG1 (arrow) was detected using the purified Pab.
IF image of A549 cell stained with FOXG1 Antibody (Center)(Cat#TA325116).A549 cells were incubated with FOXG1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . FOXG1 immunoreactivity is localized to Nucleus significantly.
FOXG1 Antibody (Center) (Cat. #TA325116) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-FOXG1A Antibody Titration: 5.0-8.0ug/ml; Positive Control: Jurkat cell lysate
Human kidney
Human Liver
WB Suggested Anti-FOXG1B Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate
Human kidney
WB Suggested Anti-FOXG1B Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
Western blot analysis of FOXG1 in rat brain tissue lysate with FOXG1 antibody at 1 ug/mL.
Immunofluorescence of FOXG1 in mouse brain tissue with FOXG1 antibody at 20 ug/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302825 | FOXG1 (C-term) antibody |
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| Goat Polyclonal Antibody against FOXG1A / FOXG1B | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA325116 | FOXG1 (Center) antibody |
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| Rabbit polyclonal FOXG1 Antibody (Center) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA349139 | FOXG1 antibody |
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| Rabbit Polyclonal Anti-FOXG1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213250L2V | Lenti ORF particles, Foxg1 (GFP-tagged ORF) - Rat forkhead box G1 (Foxg1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,160.00
|
|
| OriGene Technologies, Inc. | ||
| RR213250L1V | Lenti ORF particles, Foxg1 (Myc-DDK-tagged ORF) - Rat forkhead box G1 (Foxg1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,040.00
|
|
| OriGene Technologies, Inc. | ||