FRG1 antibody
Principal name
FRG1 antibody
Alternative names for FRG1 antibody
FSHD region gene 1 protein
Ncbi ID
9606, NP_004468, NM_013522, NP_004468.1, NM_004477, NP_038550.2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair)
Background of FRG1 antibody
- Kit Component:
- - KN306125G1, Frg1 gRNA vector 1 in pCas-Guide vector
- KN306125G2, Frg1 gRNA vector 2 in pCas-Guide vector
- KN306125D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hanel, M.L., et al. Dev. Dyn. 238(6):1502-1512(2009)
Bodega, B., et al. BMC Biol. 7, 41 (2009)
Pirozhkova, I., et al. PLoS ONE 3 (10), E3389 (2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Gabellini, D., et al. Nature 439(7079):973-977(2006)
Immunofluorescence of monoclonal antibody to FRG1 on HeLa cell. [antibody concentration 35 ug/ml]
Western Blot analysis of FRG1 expression in transfected 293T cell line by FRG1 monoclonal antibody (M01), clone 4A5.Lane 1: FRG1 transfected lysate(29 KDa).Lane 2: Non-transfected lysate.
FRG1 monoclonal antibody (M01), clone 4A5 Western Blot analysis of FRG1 expression in HeLa NE ( Cat # L013V3 ).
Western Blot analysis of FRG1 expression in transfected 293T cell line by FRG1 MaxPab rabbit polyclonal antibody.Lane 1: FRG1 transfected lysate(29.20 KDa).Lane 2: Non-transfected lysate.
Mouse Brain; WB Suggested Anti-Frg1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Mouse Brain; Frg1 antibody - middle region (ARP54731_P050) in Mouse Brain cells using Western Blot
Western blot analysis of FRG1 Antibody (C-term) Cat.-No AP51724PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the FRG1 antibody detected the FRG1 protein (arrow).
(2ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-Frg1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Mouse Brain
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311301 | FRG1 antibody |
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| Goat Anti-FRG1 Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP51724PU-N | FRG1 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308772 | FRG1 |
||||
| Recombinant protein of human FSHD region gene 1 (FRG1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417951 | FRG1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203335L2V | Lenti ORF particles, Frg1 (GFP-tagged) - Mouse FSHD region gene 1 (Frg1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203335L1V | Lenti ORF particles, Frg1 (Myc-DDK-tagged) - Mouse FSHD region gene 1 (Frg1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||