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GABARAP antibody

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Principal name

GABARAP antibody

Alternative names for GABARAP antibody

GABA Receptor Associated Protein, FLC3B, GABA(A) receptor-associated protein, Gamma-aminobutyric acid receptor-associated protein

SwissProt ID

O95166 (Human), P60517 (Rat), Q8MK68 (Rabit), Q9DCD6 (Mouse), Q9GJW7 (Bovin)

Gene ID

11337 (GABARAP), 56486 (Gabarap), 58974

Ncbi ID

NP_009209, 9606, NP_009209.1, NP_062723.1, NM_172036, NM_019749

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), Can (Canine), Ye (Yeast), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of GABARAP antibody

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABA(A) receptor-associated protein (GABARAP) is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

Stangler T., et al. (2002) J Biol Chem. 277(16):13363-6.

PAB0730 - GABARAP
Confocal microscopy on immunofluorescently detected GABARAP in cytospin-isolated human white blood cells using rabbit GABARAP polyclonal antibody ( Cat # PAB0730 ) at a dilution of 1 : 200, incubated for 1 hr at room temperature. The cells were double - stained for GABARAP appearing in red ( A ) and MHC class II appearing in green ( B ) . The cells were counter stained with Hoechst Dye ( C, blue colour ) . Here, the merged picture is presented.
PAB0730 - GABARAP
Western blot under reducing conditions on 293 cell lysate using rabbit GABARAP polyclonal antibody ( Cat # PAB0730 ) at a dilution of 1 : 100.
PAB0730 - GABARAP
Western blot under reducing conditions on Odora ( an Olfactory cell line ) cell lysate using rabbit GABARAP polyclonal antibody ( Cat # PAB0730 ) at a dilution of 1 : 100.
AR09351PU-L - GABARAP
AR09351PU-L - GABARAP
Recombinant human GABARAP, 1-117 aa, His-tagged: 15% SDS-PAGE (3 µg)
ARP51265_P050 - GABARAP
Human 721_B; WB Suggested Anti-GABARAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate; GABARAP antibody - N-terminal region (ARP51265_P050) in Human 721_B cells using Western Blot
ARP58902_P050 - GABARAP
Human Heart; WB Suggested Anti-GABARAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; GABARAP antibody - C-terminal region (ARP58902_P050) in Human Heart cells using Western Blot
ARP51266_P050 - GABARAP
Manduca sexta; . Autophagy is induced by starvation. Sample: Newborn . M. Sexta larvae. Lanes: Newborn larvae were treated for 0,0.5,1,2,3,4, and 5 h. Protein Loaded: 100 ug per lane. Submitted by: Dra. Helena Porta Ducoing, Instituto de Biotechnologia, UNAM .; GABARAP antibody - N-terminal region (ARP51266_P050) in Manduca sexta cells using Western Blot
ARP51266_P050 - GABARAP
Human HT1080; WB Suggested Anti-GABARAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HT1080 cell lysate. GABARAP is supported by BioGPS gene expression data to be expressed in HT1080.; GABARAP antibody - N-terminal region (ARP51266_P050) in Human HT1080 cells using Western Blot
AP32182PU-N - GABARAP
Formalin-Fixed, Paraffin-Embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP Antibody (N-term) Cat.-No AP32182PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP32182PU-N - GABARAP
GABARAP Antibody was tested in Mouse MEFs and Mouse motor neurons. Cells were fixed in 3% PFA and permeabilized with 100% methanol. Antibodies used were GABARAP Antibody (N-term) Cat.-No AP32182PU-N (1/500, labeled with anti-Rabbit 488 Alexa) and Covance anti-Tuj1 (1/1000, labeled with anti-Mouse 647).
AP32182PU-N - GABARAP
Immunofluorescence staining of Autophagy GABARAP Antibody (N-term) Cat.-No AP32182PU-N on paraformaldehyde-fixed, saponin-permeabilized HeLa cells. Comparison of control (full-medium) and Autophagy induced (EBSS 1 h) cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki, Finland.
AP32182PU-N - GABARAP
Western blot done using standard conditions on WT MEFs, starved with EBSS for 2 hours with and without leupeptin before lysing. Primary antibodies used were GABARAP Antibody (N-term) Cat.-No AP32182PU-N, 1/250 in 5% milk (in PBS + 0.1% Tween-20) and  Rabbit anti-Actin. Secondary detection by anti-Rabbit-HRP, ECL exposure 2 minutes (provided by Fiona McAlpine & Sharon Tooze).
AP32182PU-N - GABARAP
Western blot analysis of GABARAP (arrow) using Autophagy GABARAP Antibody (N-term) Cat.-No AP32182PU-N. 293 cell lysates (2 µg/Lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).
AP32182PU-N - GABARAP
Autophagy GABARAP Antibody (N-term) Cat.-No AP32182PU-N at 1/75 dilution in 5% scimmed milk powder. Secondary antibody Goat anti-Rabbit-HRP ECL detection 30 sec on X-ray film.
TA319834 - GABARAP
Western blot analysis of GABARAP in K562 cell lysate with GABARAP antibody at (A) 1 and (B) 2 ug/mL.
TA319834 - GABARAP
Immunofluorescence of GABARAP in human brain tissue with GABARAP antibody at 20 ug/mL.
TA332791 - GABARAP
Western blot analysis of extracts of various cell lines, using GABARAP antibody.
TA332791 - GABARAP
Immunofluorescence analysis of MCF-7 cell using GABARAP antibody. Blue: DAPI for nuclear staining.
TA336614 - GABARAP
Western Blot: GABARAP Antibody [TA336614] - Analysis of GABARAP in brain lysate using this antibody at 1:500 dilution.
TA336818 - GABARAP
Immunohistochemistry: GABARAP Antibody [TA336818] - Analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain.
TA336818 - GABARAP
Immunocytochemistry/Immunofluorescence: GABARAP Antibody [TA336818] - GABARAP antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
TA336870 - GABARAP
Immunohistochemistry: GABARAP Antibody [TA336870] - IHC analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain.
TA339168 - GABARAP
WB Suggested Anti-GABARAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate
TA339169 - GABARAP
Autophagy is induced by starvationSample: Newborn M. Sexta larvae; Lanes: Newborn larvae were treated for 0, 0.5, 1, 2, 3, 4, and 5 h; Protein Loaded: 100 ug per lane; Submitted by: Dra. Helena Porta Ducoing, Instituto de Biotechnologia, UNAM
TA339169 - GABARAP
WB Suggested Anti-GABARAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HT1080 cell lysate GABARAP is supported by BioGPS gene expression data to be expressed in HT1080
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  2. Highly Validated (1)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GABARAP (N-term) antibody
Immunofluorescence staining of Autophagy GABARAP Antibody (N-term) Cat.-No AP32182PU-N on paraformaldehyde-fixed, saponin-permeabilized HeLa cells. Comparison of control (full-medium) and Autophagy induced (EBSS 1 h) cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki, Finland. Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+5 additional images

Highly validated antibody!

GABARAP antibody
  Rabbit Polyclonal GABARAP Antibody  
Western blot analysis of GABARAP in K562 cell lysate with GABARAP antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

GABARAP antibody
  Rabbit anti-GABARAP Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using GABARAP antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

GABARAP antibody
  Rabbit Polyclonal GABARAP Antibody  
Western Blot: GABARAP Antibody [TA336614] - Analysis of GABARAP in brain lysate using this antibody at 1:500 dilution. Rabbit Purified Hu, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

GABARAP antibody
  Rabbit Polyclonal GABARAP Antibody  
Immunohistochemistry: GABARAP Antibody [TA336818] - Analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 ml / €395.00
  OriGene Technologies, Inc.
+1 additional image

GABARAP antibody
  Rabbit Polyclonal GABARAP Antibody  
Immunohistochemistry: GABARAP Antibody [TA336870] - IHC analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain. Rabbit Purified Ms C, P
0.1 ml / €395.00
  OriGene Technologies, Inc.

GABARAP antibody
GABARAP Rabbit Purified Hu, Ms, Rt WB
0.1 ml / €330.00
  Neuromics Antibodies

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gabarap (GFP-tagged) - Mouse gamma-aminobutyric acid receptor associated protein (Gabarap), 200 uL, >10^7 TU/mL
Lenti ORF particles, Gabarap (GFP-tagged) - Mouse gamma-aminobutyric acid receptor associated protein (Gabarap), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gabarap (GFP-tagged ORF) - Rat GABA(A) receptor-associated protein (Gabarap), 200 uL, >10^7 TU/mL
Lenti ORF particles, Gabarap (GFP-tagged ORF) - Rat GABA(A) receptor-associated protein (Gabarap), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Gabarap (Myc-DDK-tagged ORF) - Rat GABA(A) receptor-associated protein (Gabarap), 200 uL, >10^7 TU/mL
Lenti ORF particles, Gabarap (Myc-DDK-tagged ORF) - Rat GABA(A) receptor-associated protein (Gabarap), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

10 Item(s)

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Available Filters

Product Type
  1. Mouse Lenti ORF Particle (1)
  2. Primary Antibody (7)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (7)
Target
  1. GABARAP (7)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (5)
  3. Rat (Rt) (4)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (3)
  3. Frozen sections (C) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
Presentation
  1. Purified (6)
  2. Aff - Purified (1)
Host
  1. Rabbit (7)
Images
  1. 1 image (6)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunofluorescence (IF) (3)
  3. IHC (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  5. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (9)
  2. Neuromics Antibodies (1)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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