GABBR2 antibody
Principal name
GABBR2 antibody
Alternative names for GABBR2 antibody
GABBR-2, GPR51, GPRC3B, GABA-B receptor 2, GABA-B-R2, Gb2, G-protein coupled receptor 51, HG20, Gamma-aminobutyric acid type B receptor subunit 2
Ncbi ID
NP_005449, NP_005449.5, 9606, NP_113990.1, NM_005458, NM_001081141, NM_031802, NP_001074610.1
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoelectron Microscopy (EM), Enzyme Immunoassay (E)
Background of GABBR2 antibody
GABA (-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory syptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory syptic transmission . Functiol GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site . Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein . G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsyptic inhibitions: presyptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsyptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to syptic inhibition,GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation .
Western blotting analysis of GABA B receptor GB2 subunit in Rat liver (Lane 1), GB2-transfected HEK292 cells (Lane 2) and Rat brain (Lane 3) with polyclonal anti-GB2 antibody.
Figure 1. Western blot of rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Human HeLa; WB Suggested Anti-GABBR2 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell; GABBR2 antibody - C-terminal region (ARP61115_P050) in Human HeLa cells using Western Blot
immunoblot against adult rat brain membranes (RBM) and membranes from GABABR2 wild-type (WT) and knockout (KO) mice probed with N81/2 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Joshua Walker and Stephen Moss (Tufts)
immunoblot against crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N81/2 (left) or N52A/42 (right) TC supe.
immunoblot against adult rat brain membranes (RBM) and membranes from GABABR2 wild-type (WT) and knockout (KO) mice probed with N81/2 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Joshua Walker and Stephen Moss (Tufts)
immunoblot against crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N81/2 (left) or N52A/42 (right) TC supe.
adult rat brain immunohistochemistry
left: immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N81/37 (left) or N52A/42 (right) TC supe.
adult rat brain membrane immunoblot
immunoblot against brain membrane fractions from rat (RBM) and from GABABR2 knockout (KO) and wild-type (WT) mice probed with N81/37 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Joshua Walker and Stephen Moss (Tufts).
Electron micrograph of N81/37 labelling in stratum radiatum of mouse hippocampal CA1 using a pre-embedding immunogold method. Immunoparticles are seen at postsynaptic sites (arrows) along the extrasynaptic plasma membrane of dendritic spines (s) and shafts of pyramidal cells forming synaptic contacts with excitatory axon terminals (b), and at presynaptic sites (arrowheads) in excitatory axon terminals (b). Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).
adult rat brain immunohistochemistry
left: immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N81/37 (left) or N52A/42 (right) TC supe.
adult rat brain membrane immunoblot
immunoblot against brain membrane fractions from rat (RBM) and from GABABR2 knockout (KO) and wild-type (WT) mice probed with N81/37 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Joshua Walker and Stephen Moss (Tufts).
Electron micrograph of N81/37 labelling in stratum radiatum of mouse hippocampal CA1 using a pre-embedding immunogold method. Immunoparticles are seen at postsynaptic sites (arrows) along the extrasynaptic plasma membrane of dendritic spines (s) and shafts of pyramidal cells forming synaptic contacts with excitatory axon terminals (b), and at presynaptic sites (arrowheads) in excitatory axon terminals (b). Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).
Western blot analysis of extracts from HepG2 cells, using GABBR2 (Ab-893) Antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of rat brain membrane: 1. Anti-GABA(B) R2 antibody, (1:200). 2. Anti-GABA(B) R2 antibody preincubated with the control peptide antigen.
Expression of GABA(B) R2 in mouse hippocampus. Immunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R2 antibody, (1:100). A. GABA(B) R2 staining (green) is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red) sets apart the SLM. C. Confocal merge suggests the presence of GABA(B) R2 in layers, but not in pyramidal neurons.
Western Blot: GABA B Receptor 2 Antibody [TA336615] - Western Blot analysis of GABA BR2 in brain lysate, using TA336615.
WB Suggested Anti-GABBR2 Antibody; Titration: 1.0 ug/ml; Positive Control: Hela Whole Cell
Western blots of (A) Cos-7 cells transiently expressing GABABR2S892A (lane 1), wild type GABABR2 (lane 2) or control untransfected cells (lane 3). Blots were then immunolabeled with Rabbit Anti-Phospho Ser892 (Figure A).
Western blot of rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| 73-125 | GABBR2 antibody |
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Mouse | IgG1 | N81/37 | Supernatant | Hu, Ms, Rt | EM, ICC/IF, WB |
5 ml /
€490.00
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| Antibodies Incorporated | |||||||
 +4 additional images |
Highly validated antibody!
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| 75-125 | GABBR2 antibody |
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 |
Mouse | IgG1 | N81/37 | Purified | Hu, Ms, Rt | EM, ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +4 additional images |
Highly validated antibody!
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| TA309142 | GABBR2 antibody |
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| Rabbit Anti-GABAB Receptor (Ser783), R2-Subunit Antibody (Phospho-Specific) | |||||||
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Rabbit | IgG | Purified | Rt | ICC/IF, WB |
0.1 ml /
€405.00
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| OriGene Technologies, Inc. | |||||||
| TA312880 | GABBR2 antibody |
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| Rabbit polyclonal GABBR2 (Ab-893) antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA326531 | GABBR2 antibody |
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| Mouse Monoclonal Anti-GABA-B Receptor 2 Antibody | |||||||
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Mouse | IgG1 | S81-2 | Purified | Hu, Ms, Rt |
0.1 mg /
€410.00
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| OriGene Technologies, Inc. | |||||||
| TA328675 | GABBR2 antibody |
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| Rabbit Polyclonal Anti-GABA (B) R2 | |||||||
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Rabbit | Purified | Hu, Ms, Rt | WB |
0.2 ml /
€585.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336615 | GABBR2 antibody |
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| Rabbit Polyclonal GABA-B R2 Antibody | |||||||
![Western Blot: GABA B Receptor 2 Antibody [TA336615] - Western Blot analysis of GABA BR2 in brain lysate, using TA336615.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB300-159-1-W.jpg) |
Rabbit | Purified | Hu | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| 73-124 | GABBR2 antibody |
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 |
Mouse | IgG2a | N81/2 | Supernatant | Hu, Ms, Rt | C, ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
| +1 additional image |
|
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| 75-124 | GABBR2 antibody |
||||||
 |
Mouse | IgG2a | N81/2 | Purified | Hu, Ms, Rt | C, ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +1 additional image |
|
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| AP03015PU-N | GABBR2 antibody |
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Rabbit | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€260.00
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| OriGene Technologies GmbH | |||||||
| GT15009-100 | GABBR2 antibody |
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Goat | Aff - Purified | Rt | C, ICC/IF, P, WB |
0.1 mg /
€450.00
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| Neuromics Antibodies | |||||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214644L2V | Lenti ORF particles, Gabbr2 (GFP-tagged ORF) - Rat gamma-aminobutyric acid (GABA) B receptor 2 (Gabbr2), 200 uL, >10^7 TU/mL |
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200 µl /
€1,800.00
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| OriGene Technologies, Inc. | ||