GABPB1 antibody
Principal name
GABPB1 antibody
Alternative names for GABPB1 antibody
E4TF1B, GABPB, GABPB2, GABP subunit beta-2, GABPB-2, Nuclear respiratory factor 2, E4TF1-47, E4TF1-53, GA-binding protein subunit beta-1, Nuclear respiratory factor 2, Transcription factor E4TF1-47, Transcription factor E4TF1-53
Ncbi ID
9606, NP_005245, NP_002032, NP_002032.2, NM_005254, NM_002041, NP_034379.1, NM_016654, NM_016655, NM_181427
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of GABPB1 antibody
GABPB2 is the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
General readings
Sawada,J., et al., (1999) J. Biol. Chem. 274 (50), 35475-35482
GABPB2 polyclonal antibody (A01), Lot # 060703JCS1 Western Blot analysis of GABPB2 expression in 293 ( Cat # L026V1 ).
Human HepG2; WB Suggested Antibody Titration: 1.25 ug/ml. Positive Control: HepG2. GABPB1 is supported by BioGPS gene expression data to be expressed in HepG2.; GABPB2 antibody - C-terminal region (P100925_T100) in Human HepG2 cells using Western Blot
Human Spleen; GABPB2 antibody - C-terminal region (P100925_P050) in Human Spleen cells using Immunohistochemistry
Human Lung; GABPB2 antibody - C-terminal region (P100925_P050) in Human Lung cells using Immunohistochemistry
Human Kidney; . Lanes: 1. 40 ug human HK2 cell (kidney proximal tubular cell line) lysate. 2. 40 ug H2O2 treated human HK2 Cell lysate. 3. 40 ug H2O2 treated human HK2 Cell lysate. 4. 40 ug H2O2 treated human HK2 Cell lysate. 5. 40 ug H2O2 treated human HK2 Cell lysate. 6. 40 ug H2O2 treated human HK2 Cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-Rabbit HRP. Secondary Antibody Dilution: 1:2000. Gene Name: GABPB2. Submitted by: The University of Queensland, School of Medicine, Centre for Kidney Disease Research.; GABPB2 antibody - C-terminal region (P100925_P050) in Human Kidney cells using Western Blot
Human HepG2; WB Suggested Anti-GABPB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. GABPB1 is supported by BioGPS gene expression data to be expressed in HepG2.; GABPB2 antibody - C-terminal region (P100925_P050) in Human HepG2 cells using Western Blot
GABPB2 polyclonal antibody ( Cat # PAB7286 ) ( 0.5 µg/ml ) staining of HeLa cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoperoxidase of purified MaxPab antibody to GABPB2 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to GABPB2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of GABPB1 expression in transfected 293T cell line (H00002553-T01) by GABPB1 MaxPab polyclonal antibody.Lane 1: GABPB2 transfected lysate(43.45 KDa).Lane 2: Non-transfected lysate.
GABPB1 MaxPab polyclonal antibody. Western Blot analysis of GABPB1 expression in Jurkat.
GABPB2 MaxPab polyclonal antibody. Western Blot analysis of GABPB2 expression in Hela NE.
GABPB1 MaxPab polyclonal antibody. Western Blot analysis of GABPB1 expression in PC-12.
Immunoperoxidase of purified MaxPab antibody to GABPB2 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to GABPB2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of GABPB1 expression in transfected 293T cell line (H00002553-T01) by GABPB1 MaxPab polyclonal antibody.Lane 1: GABPB2 transfected lysate(43.45 KDa).Lane 2: Non-transfected lysate.
GABPB1 MaxPab polyclonal antibody. Western Blot analysis of GABPB1 expression in Jurkat.
GABPB2 MaxPab polyclonal antibody. Western Blot analysis of GABPB2 expression in Hela NE.
GABPB1 MaxPab polyclonal antibody. Western Blot analysis of GABPB1 expression in PC-12.
Human Muscle; WB Suggested Anti-GABPB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; GABPB2 antibody - C-terminal region (P100833_P050) in Human Muscle cells using Western Blot
Western Blot analysis of GABPB1 expression in transfected 293T cell line (H00002553-T02) by GABPB1 MaxPab polyclonal antibody.Lane 1: GABPB2 transfected lysate(42.50 KDa).Lane 2: Non-transfected lysate.
Transfected 293T; WB Suggested Anti-GABPB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; GABPB2 antibody - N-terminal region (ARP57863_P050) in Transfected 293T cells using Western Blot
Immunoprecipitation of GABPB1 transfected lysate using anti-GABPB1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GABPB1 MaxPab mouse polyclonal antibody (B01) (H00002553-B01).
Human HeLa; WB Suggested Anti-GABPB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate. GABPB1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; GABPB2 antibody - middle region (ARP31189_P050) in Human HeLa cells using Western Blot
Human GABPB1 recombinant protein
Human GABPB1 recombinant protein
Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. TA308482 diluted at 1:5000.
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using GABP-beta1(TA308482) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GABPB1(TA308482) antibody at 1:500 dilution.
WB Suggested Anti-GABPB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle
WB Suggested Anti-GABPB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysateGABPB1 is supported by BioGPS gene expression data to be expressed in HepG2
Lanes: 1. 40 ug human HK2 cell (kidney proximal tubular cell line) lysate; 2. 40 ug H2O2 treated human HK2 Cell lysate; 3. 40 ug H2O2 treated human HK2 Cell lysate; 4. 40 ug H2O2 treated human HK2 Cell lysate; 5. 40 ug H2O2 treated human HK2 Cell lysate;
Human Lung
Human Spleen
WB Suggested Antibody Titration: 1.25 ug/ml; Positive Control: HepG2GABPB1 is supported by BioGPS gene expression data to be expressed in HepG2
WB Suggested Anti-GABPB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: MCF7 cells, 231 cells, Primary antibody: (GABPB2 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (GABPB2 Antibody) at dilution 1/30. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human brain tissue using (GABPB2 Antibody) at dilution 1/30. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308482 | GABPB1 antibody |
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| Rabbit Polyclonal antibody to GABPB1 (GA binding protein transcription factor, beta subunit 1) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA350001 | GABPB1 antibody |
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| Rabbit Polyclonal Anti-GABPB2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760093 | GABPB1 (full length, N-term HIS tag, transcript variant gamma-1) |
||||
| Recombinant protein of human GA binding protein transcription factor, beta subunit 1 (GABPB1), transcript variant gamma-1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760274 | GABPB1 (full length, N-term HIS tag, transcript variant beta-1) |
||||
| Recombinant protein of human GA binding protein transcription factor, beta subunit 1 (GABPB1), transcript variant beta-1 , full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401615 | GABPB1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY419570 | GABPB1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
