GADD45A antibody
Principal name
GADD45A antibody
Alternative names for GADD45A antibody
GADD45, DDIT1, DDIT-1, Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein
Ncbi ID
9606, NP_001915, NM_001924, NP_001915.1, NP_031862.1, NM_007836
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of GADD45A antibody
- Kit Component:
- - KN204005G1, GADD45A gRNA vector 1 in pCas-Guide vector
- KN204005G2, GADD45A gRNA vector 2 in pCas-Guide vector
- KN204005D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of anti-GADD45A Antibody (N-term) (Cat.#AP12434PU-N) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.
Western blot analysis of GADD45A Antibody (C-term) (AP18028PU-N) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.
Recombinant human GADD45A, 1-165 aa, His-tagged: 15% SDS-PAGE (3 ug)
Human Heart; WB Suggested Anti-GADD45A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human heart; GADD45A antibody - C-terminal region (ARP60167_P050) in Human Heart cells using Western Blot
Western Blot analysis of GADD45A expression in transfected 293T cell line (H00001647-T01) by GADD45A MaxPab polyclonal antibody.Lane 1: GADD45A transfected lysate(18.30 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP2K6 and GADD45A HeLa cells were stained with anti-MAP2K6 rabbit purified polyclonal 1:1200 and anti-GADD45A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (RC204005, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (RC204005, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.
WB Suggested Anti-GADD45A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human heart
Western blot detection of GADD45 a in CHO-K1 cell lysate(B)and CHO-K1 transfected by GADD45 a His fusion protein(A)cell lysate using GADD45 a mouse mAb (1:1000 diluted). Predicted band size:22KDa. Observed band size:22KDa.
Western blot analysis of extracts of various cell lines, using GADD45A antibody.
Immunohistochemistry of paraffin-embedded human esophagus using GADD45A antibody at dilution of 1:100 (400x lens).
The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (GADD45A Antibody) at dilution 1/30. (Original magnification: ×200)
- Quick-Filters:
- Overnight Delivery (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332434 | GADD45A antibody |
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| Rabbit anti-GADD45A Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA346952 | GADD45A antibody |
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| Mouse Monoclonal GADD45a Antibody | |||||||
 |
Mouse | IgG1 | 6C3-G3-E9 | Purified | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350003 | GADD45A antibody |
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| Rabbit Polyclonal Anti-GADD45A Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA507370 | GADD45A antibody |
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| GADD45A mouse monoclonal antibody, clone OTI1C9 (formerly 1C9) | |||||||
 |
Mouse | IgG2b | OTI1C9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA507380 | GADD45A antibody |
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| GADD45A mouse monoclonal antibody, clone OTI2B7 (formerly 2B7) | |||||||
 |
Mouse | IgG1 | OTI2B7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP12434PU-N | GADD45A (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP18028PU-N | GADD45A (C-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304005 | GADD45A |
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| Recombinant protein of human growth arrest and DNA-damage-inducible, alpha (GADD45A) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720561 | GADD45A |
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| Recombinant protein of human growth arrest and DNA-damage-inducible, alpha (GADD45A) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419659 | GADD45A overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201332L2V | Lenti ORF particles, Gadd45a (GFP-tagged) - Mouse growth arrest and DNA-damage-inducible 45 alpha (Gadd45a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201332L1V | Lenti ORF particles, Gadd45a (Myc-DDK-tagged) - Mouse growth arrest and DNA-damage-inducible 45 alpha (Gadd45a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
