GADD45G antibody
Principal name
GADD45G antibody
Alternative names for GADD45G antibody
CR6, DDIT2, Growth arrest and DNA-damage-inducible protein GADD45 gamma, Cytokine-responsive protein CR6, DNA-damage-inducible transcript 2
Ncbi ID
9606, NP_006696.1, NP_006696, NM_006705, 10090, NM_001077640
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of GADD45G antibody
- Kit Component:
- - KN306259G1, Gadd45g gRNA vector 1 in pCas-Guide vector
- KN306259G2, Gadd45g gRNA vector 2 in pCas-Guide vector
- KN306259D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Flores, O., et al. Endocrinology 151(10):4654-4664(2010)
Zhang, W., et al. J. Cancer Res. Clin. Oncol. 136(8):1267-1273(2010)
Zhu, N., et al. Mol. Biol. Rep. 36(8):2075-2085(2009)
Yu, S., et al. Am. J. Nephrol. 30(2):135-139(2009)
Palmieri, R.T., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3567-3572(2008)
Detection limit for recombinant GST tagged GADD45G is approximately 0.03ng/ml as a capture antibody.
Recombinant GADD45G, 1-159 aa: 15 % SDS-PAGE (3 µg)
Immunofluorescence of purified MaxPab antibody to GADD45G on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of GADD45G expression in transfected 293T cell line (H00010912-T01) by GADD45G MaxPab polyclonal antibody.Lane 1: GADD45G transfected lysate(17.49 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of GADD45G Antibody (N-term) Cat.-No AP51756PU-N in A549 cell line lysates (35ug/lane). This demonstrates the GADD45G antibody detected the GADD45G protein (arrow).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505437)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505437)
Anti-GADD45G mouse monoclonal antibody (TA505437) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G(RC201364).
Figure from citation: Western Blot of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines. View Citation
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human CRC cells. View Citation
Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human GBM8401 cells. View Citation
Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines. View Citation
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505439)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505441)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505478)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505478)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505480)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505480)
Anti-GADD45G mouse monoclonal antibody (TA505480) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G(RC201364).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505484)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Anti-GADD45G mouse monoclonal antibody (TA505489) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G(RC201364).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells transfected with either RC201364 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GADD45G antibody(TA505489), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Anti-GADD45G mouse monoclonal antibody (TA505491) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G(RC201364).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells transfected with either RC201364 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GADD45G antibody(TA505491), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Anti-GADD45G mouse monoclonal antibody (TA505494) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G(RC201364).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505500)
Anti-GADD45G mouse monoclonal antibody (TA505500) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G(RC201364).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
Sample (30 ug of whole cell lysate). A: Hela. 12% SDS PAGE. TA308278 diluted at 1:1000.
Immunofluorescence analysis of methanol-fixed HeLa, using GADD45 gamma(TA308278) antibody at 1:200 dilution.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using GA45G antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using GA45G antibody.The picture on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/100-200 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using GADD45G antibody at dilution 1/120, on the right is treated with the fusion protein. (Original magnification: x200)
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- Cited (1)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505437 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI2F12 (formerly 2F12) | |||||||
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Mouse | IgG1 | OTI2F12 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Top Selling product! Published recently! Highly validated antibody!
|
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| TA505480 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI1H10 (formerly 1H10) | |||||||
 |
Mouse | IgG2b | OTI1H10 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
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| TA308278 | GADD45G antibody |
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| Rabbit Polyclonal antibody to GADD45 gamma (growth arrest and DNA-damage-inducible, gamma) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312792 | GADD45G antibody |
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| Rabbit polyclonal anti-GA45G antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | ICC/IF |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA322475 | GADD45G antibody |
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| Anti-GADD45G Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA505421 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI3A1 (formerly 3A1) | |||||||
 |
Mouse | IgG1 | OTI3A1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA505439 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI2H6 (formerly 2H6) | |||||||
 |
Mouse | IgG1 | OTI2H6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA505440 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI4D6 (formerly 4D6) | |||||||
 |
Mouse | IgG1 | OTI4D6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA505441 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTIA12 (formerly A12) | |||||||
 |
Mouse | IgG1 | OTIA12 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA505476 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI2D4 | |||||||
 |
Mouse | IgG1 | OTI2D4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA505477 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI1B7 | |||||||
 |
Mouse | IgG1 | OTI1B7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA505478 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI2G6 (formerly 2G6) | |||||||
 |
Mouse | IgG1 | OTI2G6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA505479 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI2C1 (formerly 2C1) | |||||||
 |
Mouse | IgG1 | OTI2C1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA505482 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI1F2 (formerly 1F2) | |||||||
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Mouse | IgG1 | OTI1F2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA505484 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI1D10 (formerly 1D10) | |||||||
 |
Mouse | IgG2a | OTI1D10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA505486 | GADD45G antibody |
||||||
| GADD45G mouse monoclonal antibody, clone OTI2D7 (formerly 2D7) | |||||||
 |
Mouse | IgG1 | OTI2D7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA505487 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI2A7 (formerly 2A7) | |||||||
 |
Mouse | IgG2a | OTI2A7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA505488 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI7G10 (formerly 7G10) | |||||||
 |
Mouse | IgG1 | OTI7G10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA505489 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI1E4 (formerly 1E4) | |||||||
 |
Mouse | IgG2a | OTI1E4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA505490 | GADD45G antibody |
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| GADD45G mouse monoclonal antibody, clone OTI1H1 (formerly 1H1) | |||||||
 |
Mouse | IgG2a | OTI1H1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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