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Galectin-1 antibody

Principal name

Galectin-1 antibody

Alternative names for Galectin-1 antibody

LGALS1, Galectin1, Lectin galactoside-binding soluble 1, Beta-galactoside-binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin 1, Galaptin, 14 kDa lectin, HPL, HBL, MAPK-activating protein PM12

SwissProt ID

P09382 (Human), P11116 (Bovin), P11762 (Rat), P16045 (Mouse), P81184 (Sheep), Q49I35 (Pig)

Gene ID

3956 (LGALS1), 16852 (Lgals1), 56646

Ncbi ID

NP_002296.1, 9606, NP_032521.1, NP_002296, NP_032521, NM_002305, NM_008495, NM_019904

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Rt (Rat), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Rat, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F)

Background of Galectin-1 antibody

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse.[4] They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

General readings

Okano, K., et al. Exp. Cell Res. 316(19):3282-3291(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Pal, Z., et al. J. Neuroimmunol. (2010) In press
Tang, C.E., et al. Oncol. Rep. 24(2):495-500(2010)
Garner, O.B., et al. PLoS Pathog. 6 (7), E1000993 (2010)

Western blot analysis with 250 ng His-tagged recombinant human Galectin-1 protein.

AP00267PU-N

Immunofluorescence of monoclonal antibody to LGALS1 on HeLa cell. [antibody concentration 10 ug/ml]

H00003956-M01

Western Blot analysis of LGALS1 expression in transfected 293T cell line by LGALS1 monoclonal antibody (M01), clone 1E8-1B2.Lane 1: LGALS1 transfected lysate(14.7 KDa).Lane 2: Non-transfected lysate.

H00003956-M01

LGALS1 monoclonal antibody (M01), clone 1E8-1B2 Western Blot analysis of LGALS1 expression in HepG2 ( Cat # L019V1 ).

H00003956-M01

LGALS1 MaxPab rabbit polyclonal antibody. Western Blot analysis of LGALS1 expression in mouse spleen.

H00003956-D01P

LGALS1 MaxPab rabbit polyclonal antibody. Western Blot analysis of LGALS1 expression in human kidney.

H00003956-D01P

Western Blot analysis of LGALS1 expression in transfected 293T cell line (H00003956-T01) by LGALS1 MaxPab polyclonal antibody.Lane 1: LGALS1 transfected lysate(14.70 KDa).Lane 2: Non-transfected lysate.

H00003956-D01P

LGALS1 MaxPab rabbit polyclonal antibody. Western Blot analysis of LGALS1 expression in HeLa.

H00003956-D01P

Figure 1. Western blot analysis of GLT Monoclonal Antibody (Cat. #AM11026SU-N) in A375 cell line lysates. GLT (arrow) was detected using the ascites Mab. (dilution 1/1000).

AM11026SU-N

Recombinant human Galectin-1, 1-135 aa: 15% SDS-PAGE (3 µg)

Western blot analysis of LGALS1 using 250 ng His-tagged recombinant human LGALS1.

PAB8735

Mouse macrophages; LGALS1 antibody - middle region (ARP58491_P050) in Mouse macrophages cells using Western Blot

ARP58491_P050

Human Muscle; WB Suggested Anti-LGALS1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; LGALS1 antibody - middle region (ARP58491_P050) in Human Muscle cells using Western Blot

ARP58491_P050

AP23675PU-N Galectin-1 antibody staining of Mouse (A) and Rat (B) Pancreas lysates and NIH3T3 (C) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP23675PU-N

Western blot analysis of Galectin-1 Antibody Cat.-No AP51860PU-N in U251 cell line lysates (35ug/lane).

AP51860PU-N

Human placenta stained with anti-Galectin-1 antibody Cat.-No AM33389PU (Clone SP247)

Western Blot analysis of NIH3T3 cell lysate with Galectin-1 antibody Cat.-No AM33389PU (Clone SP247)

Flow Cytometric analysis of Rabbit anti-Galectin-1 antibody Cat.-No AM33389PU (Clone SP247) in HL-60 cells (green) compare to negative control of Rabbit IgG (blue)

Western blot analysis of Galectin 1 expression in HeLa (A); HepG2 (B); Raw264.7 (C); H9C2 (D); Human liver (E) whole cell lysates.

Representative Standard Curve

CEK1465

TP304674

TP723123

(0.5ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA311389

Western blot analysis of extracts from K562/NIH-3T3 cells, using LGALS1 antibody.The lane on the right is treated with the synthesized peptide.

TA311982

Gel: 12+15%SDS-PAGE, Lysate: 40 µg, Lane: Hela cells, Primary antibody: LGALS1 Antibody at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds

TA321466

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using LGALS1 Antibody at dilution 1/25, on the right is treated with fusion protein. (Original magnification:×200)

TA321466

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using LGALS1 Antibody at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)

TA321466

Predicted band size: 15 kDa . Positive control:Human fetal muscle tissue lysate. Recommended dilution: 1/500-2000. (Gel: 15%+12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)

TA321467

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using LGALS1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200)

TA321467

WB Suggested Anti-LGALS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle

TA344315

Mouse macrophages

TA344315

Western Blot analysis of NIH3T3 cell lysate with galectin-1 antibody

TA353837

Human placenta stained with anti-galectin-1 antibody.

TA353837

Flow cytometric analysis of rabbit anti-Galectin-1 (SP247) antibody in HL-60 cells (green) compare to negative control of rabbit IgG (blue).

TA353837

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AP23675PU-N	Galectin-1 (N-term) antibody
	Goat			Aff - Purified	Bov, Hu, Ms, Por, Rt	E, WB	0.1 mg / €380.00
							OriGene Technologies GmbH
	OriGene recommends this product for Western blotting.
TA311389	Galectin-1 (100-112) antibody
	Goat Anti-Lgals1 / galectin-1 (aa100-112) Antibody
	Goat			Aff - Purified	Bov, Hu, Ms, Rt	E, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA311982	Galectin-1 antibody
	Rabbit polyclonal anti-LGALS1 antibody
	Rabbit	IgG		Purified	Hu, Rt	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA321466	Galectin-1 antibody
	Anti-LGALS1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA321467	Galectin-1 antibody
	Anti-LGALS1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
AM11026SU-N	Galectin-1 antibody
	Mouse	IgG1	24CT661.4.4	Ascites	Hu	WB	0.1 ml / €415.00
							OriGene Technologies GmbH
AP00267PU-N	Galectin-1 antibody
	Rabbit			Aff - Purified	Hu	WB	0.1 mg / €355.00
							OriGene Technologies GmbH
AP51860PU-N	Galectin-1 antibody
	Rabbit	Ig		Aff - Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies GmbH
DM3545P	Galectin-1 antibody
	Rat	IgG2	9J26	Purified	Ms	C, WB	0.1 mg / €460.00
							OriGene Technologies GmbH
PP1223B1	Galectin-1 antibody
	Rabbit			Biotin	Hu	E, WB	25 µg / €250.00
							OriGene Technologies GmbH
PP1223B2	Galectin-1 antibody
	Rabbit			Biotin	Hu	E, WB	50 µg / €340.00
							OriGene Technologies GmbH
PP1223P1	Galectin-1 antibody
	Rabbit			Aff - Purified	Hu	E, WB	50 µg / €250.00
							OriGene Technologies GmbH
PP1223P2	Galectin-1 antibody
	Rabbit			Aff - Purified	Hu	E, WB	0.1 mg / €340.00
							OriGene Technologies GmbH

CRISPR

Catalog No.	Species	Donor Vector
KN204674	LGALS1 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN204674BN	LGALS1 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN309240	Lgals1 - mouse gene knockout kit via CRISPR
	Mouse	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN309240BN	Lgals1 - mouse gene knockout kit via CRISPR
	Mouse	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN309240LP	Lgals1 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN309240RB	Lgals1 - mouse gene knockout kit via CRISPR
	Mouse	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP304674	Galectin-1
	Recombinant protein of human lectin, galactoside-binding, soluble, 1 (LGALS1)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

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