Galectin-3-binding protein antibody
Principal name
Galectin-3-binding protein antibody
Alternative names for Galectin-3-binding protein antibody
MAC2BP, Tumor-associated antigen 90K, LGALS3BP, M2BP, Lectin galactoside-binding soluble 3-binding protein,vBasement membrane autoantigen p105, Mac-2-binding protein
Ncbi ID
9606, NP_005558, NP_005558.1, NM_005567, NP_035280.1, NM_011150
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)
Background of Galectin-3-binding protein antibody
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Lee,Y.J., Clin. Exp. Rheumatol. 25 (4 SUPPL 45), S41-S45 (2007)
Western Blot analysis of LGALS3BP expression in transfected 293T cell line (H00003959-T01) by LGALS3BP MaxPab polyclonal antibody.Lane 1: LGALS3BP transfected lysate(64.35 KDa).Lane 2: Non-transfected lysate.
LGALS3BP MaxPab polyclonal antibody. Western Blot analysis of LGALS3BP expression in A-431.
Human Brain; WB Suggested Anti-LGALS3BP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; LGALS3BP antibody - middle region (ARP54779_P050) in Human Brain cells using Western Blot
Formalin-Fixed, Paraffin-Embedded Human Small Intestine
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP Antibody.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGALS3BP Antibody, and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503453)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503453)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503453)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503453)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503453)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LGALS3BP monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.
HEK293T cells transfected with either RC204918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGALS3BP antibody(TA503453), and then analyzed by flow cytometry.
Anti-LGALS3BP mouse monoclonal antibody (TA503454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGALS3BP(RC204918).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-LGALS3BP monoclonal antibody.(1:200)
HEK293T cells transfected with either RC204918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGALS3BP antibody(TA503454), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503455)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503455)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503455)
Anti-LGALS3BP mouse monoclonal antibody (TA503455) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGALS3BP(RC204918).
Western blot analysis of extracts (10ug) from 1 cell line by using anti-LGALS3BP monoclonal antibody at 1:200 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.
HEK293T cells transfected with either RC204918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGALS3BP antibody(TA503455), and then analyzed by flow cytometry.
Anti-LGALS3BP mouse monoclonal antibody (TA503456) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGALS3BP(RC204918).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.
HEK293T cells transfected with either RC204918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGALS3BP antibody(TA503456), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503457)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503457)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503457)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503457)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503457)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LGALS3BP monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.
HEK293T cells transfected with either RC204918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGALS3BP antibody(TA503457), and then analyzed by flow cytometry.
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-LGALS3BP monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.
HEK293T cells transfected with either RC204918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGALS3BP antibody(TA503459), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503500)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503500)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503500)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGALS3BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503500)
Anti-LGALS3BP mouse monoclonal antibody (TA503500) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGALS3BP(RC204918).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.
HEK293T cells transfected with either RC204918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGALS3BP antibody(TA503500), and then analyzed by flow cytometry.
WB Suggested Anti-LGALS3BP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human brain
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (LGALS3BP Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (LGALS3BP Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA350135 | Galectin-3-binding protein antibody |
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| Rabbit Polyclonal Anti-LGALS3BP Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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