GALNS antibody
Principal name
GALNS antibody
Alternative names for GALNS antibody
N-acetylgalactosamine-6-sulfatase, EC=3.1.6.4, N-acetylgalactosamine-6-sulfate sulfatase, Galactose-6-sulfate sulfatase, GalNAc6S sulfatase, Chondroitinsulfatase, Chondroitinase
Ncbi ID
9606, NP_000503.1, NP_000503, NP_001180574.1, NM_016722, NM_001193645, NM_001047851, NM_000512
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of GALNS antibody
- Kit Component:
- - KN306273G1, Galns gRNA vector 1 in pCas-Guide vector
- KN306273G2, Galns gRNA vector 2 in pCas-Guide vector
- KN306273D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Houle JD, Tom VJ, Mayes D, Wagoner G, Phillips N, Silver J.
Combining an autologous peripheral nervous system "bridge" and matrix modification by chondroitinase allows robust, functional regeneration beyond a hemisection lesion of the adult rat spinal cord.
J Neurosci. 2006 Jul 12;26(28):7405-15.
PMID: 16837588
GALNS polyclonal antibody ( Cat # PAB7554 ) ( 0.01 µg/ml ) staining of human bone marrow lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of GALNS expression in transfected 293T cell line (H00002588-T01) by GALNS MaxPab polyclonal antibody.Lane1:GALNS transfected lysate(57.42 KDa).Lane2:Non-transfected lysate.
TA303287 (0.01µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. TA308487 diluted at 1:1000
GALNS antibody detects GALNS protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug C2C12 whole cell lysate/extract. 7.5 % SDS-PAGE. GALNS antibody (TA308487) dilution: 1:1000
GALNS antibody detects GALNS protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 7.5 % SDS-PAGE. GALNS antibody (TA308487) dilution: 1:500
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GALNS(TA308487) antibody at 1:100 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS(TA308487) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:500.
GALNS Antibody (Center) (Cat. #TA324418) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the GALNS antibody detected the GALNS protein (arrow).
GALNS Antibody (Center) (Cat. #TA324418)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GALNS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of GALNS Antibody (Center)(Cat#TA324418) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
GALNS Antibody (Center) (Cat. #TA324418) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts of various cell lines, using GALNS antibody.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308487 | GALNS antibody |
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| Rabbit Polyclonal antibody to GALNS (galactosamine (N-acetyl)-6-sulfate sulfatase) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA303287 | GALNS antibody |
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| Goat Polyclonal Antibody against GALNS | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA324418 | GALNS (Center) antibody |
||||||
| Rabbit polyclonal GALNS Antibody (Center) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
|||||||
| TA332700 | GALNS antibody |
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| Rabbit anti-GALNS Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308708 | GALNS |
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| Recombinant protein of human galactosamine (N-acetyl)-6-sulfate sulfatase (GALNS) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208325L2V | Lenti ORF particles, Galns (GFP-tagged) - Mouse galactosamine (N-acetyl)-6-sulfate sulfatase (Galns), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,220.00
|
|
| OriGene Technologies, Inc. | ||
| MR208325L1V | Lenti ORF particles, Galns (Myc-DDK-tagged) - Mouse galactosamine (N-acetyl)-6-sulfate sulfatase (Galns), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,090.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201689L2V | Lenti ORF particles, Galns (GFP-tagged ORF) - Rat galactosamine (N-acetyl)-6-sulfate sulfatase (Galns), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,220.00
|
|
| OriGene Technologies, Inc. | ||
| RR201689L1V | Lenti ORF particles, Galns (Myc-DDK-tagged ORF) - Rat galactosamine (N-acetyl)-6-sulfate sulfatase (Galns), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,090.00
|
|
| OriGene Technologies, Inc. | ||