GALR2 antibody
Principal name
GALR2 antibody
Alternative names for GALR2 antibody
GALR-2, GALNR2, GAL2-R, Galanin receptor type 2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of GALR2 antibody
Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30 amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7 transmembrane family of G protein coupled receptors. GALR2 interacts with the N terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways.GalR2 expression has been documented widely in the brain and in peripheral tissues including small intestine, heart, kidney, and liver. Localization data from rat CNS indicate high levels of expression in dorsal root ganglia, amygdala and Purkinje neurons.
General readings
Berger A, Tuechler C, Almer D, Kogner P, Ratschek M, Kerbl R, Iismaa TP, Jones N, Sperl W, Kofler B.
Elevated expression of galanin receptors in childhood neuroblastic tumors.
Neuroendocrinology. 2002 Feb;75(2):130-8.
PMID: 11867941
GALR2 polyclonal antibody ( Cat # PAB7174 ) ( 0.3 µg/ml ) staining of human heart lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of GALR2 antibody Cat.-No AP01317PU-N in RAW264.7 whole cell lysate at 1/500 dilution.
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot: Sample (30 µg whole cell lysate). A:293T. B:H1299. 10% SDS PAGE. GALR2 antibody diluted at 1:1000.
Human A549; WB Suggested Anti-GALR2 Antibody. . Titration: 1.0 ug/ml. . Positive Control: A549 Whole Cell. GALR2 is supported by BioGPS gene expression data to be expressed in A549.; GALR2 antibody - C-terminal region (ARP61395_P050) in Human A549 cells using Western Blot
Western blot analysis of GALR2 expression in A431 (A) whole cell lysates.
TA303179 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample(30 ug whole cell lysate). A:293T. B:H1299. 10% SDS PAGE. TA308347 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Skin, using Galanin Receptor 2(TA308347) antibody(10 ug/ml).
Sample(30 ug of whole cell lysate). A:A431. 12% SDS PAGE. TA308348 diluted at 1:500
Galanin Receptor 2 antibody detects GALR2 protein at membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Galanin Receptor 2 antibody (TA308348) dilution: 1:500.
Galanin Receptor 2 antibody detects Galanin Receptor 2 protein at membrane and cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Galanin Receptor 2 antibody (TA308348) dilution: 1:500.
Anti-GALR2 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GALR2 / Galanin Receptor 2 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GALR2 / Galanin Receptor 2 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GALR2 / Galanin Receptor 2 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GALR2 / Galanin Receptor 2 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GALR2 / Galanin Receptor 2 antibody IHC of human skin, melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GALR2 / Galanin Receptor 2 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GALR2 / Galanin Receptor 2 antibody IHC of human skin hair follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GALR2 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GALR2 / Galanin Receptor 2 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western blot analysis of rat hippocampus, DRG (lanes 2 and 5), brain (lanes 3 and 6), mouse brain (lanes 7 and 9) and human prostate carcinoma LNCaP cell lysates: 1, 2, 3, 7, 8. Anti-Galanin Receptor Type 2 antibody, (1:200). 4, 5, 6, 9, 10. Anti-Galanin Receptor Type 2 antibody, preincubated with the control peptide antigen.
Expression of GALR2 in rat DRG. Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Galanin Receptor Type 2 antibody, (1:100). GALR2 is expressed in the DRG neurons. Hoechst 33342 is used as the counterstain.
Western blot analysis of GALR2 expression in mouse brain (A); rat brain (B) whole cell lysates.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP01317PU-N | GALR2 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
Published recently! |
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| TA303179 | GALR2 antibody |
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| Goat Polyclonal Antibody against GALR2 | |||||||
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Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA308347 | GALR2 antibody |
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| Rabbit Polyclonal antibody to Galanin Receptor 2 (galanin receptor 2) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA308348 | GALR2 antibody |
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| Rabbit polyclonal antibody to galanin receptor 2 (galanin receptor 2) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA317559 | GALR2 (C-term) antibody |
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| GALR2 Rabbit Polyclonal (C-Terminus) Antibody | |||||||
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Rabbit | Aff - Purified | Hu | P |
50 µg /
€425.00
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| OriGene Technologies, Inc. | |||||||
| TA317560 | GALR2 antibody |
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| GALR2 Rabbit Polyclonal (Transmembrane Domain) Antibody | |||||||
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Rabbit | Aff - Purified | Hu | P |
50 µg /
€425.00
|
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| OriGene Technologies, Inc. | |||||||
| TA328850 | GALR2 antibody |
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| Rabbit Polyclonal Anti-Galanin Receptor Type 2 | |||||||
 |
Rabbit | Purified | Hu, Ms, Rt | WB |
0.2 ml /
€585.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP23091PU-N | GALR2 antibody |
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Rabbit | Purified | Hu | P, WB |
50 µl /
€425.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN222436 | GALR2 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN222436BN | GALR2 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN222436LP | GALR2 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN222436RB | GALR2 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR217332L2V | Lenti ORF particles, Galr2 (GFP-tagged) - Mouse galanin receptor 2 (Galr2), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| MR217332L1V | Lenti ORF particles, Galr2 (Myc-DDK-tagged) - Mouse galanin receptor 2 (Galr2), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR200661L2V | Lenti ORF particles, Galr2 (GFP-tagged ORF) - Rat galanin receptor 2 (Galr2), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| RR200661L1V | Lenti ORF particles, Galr2 (Myc-DDK-tagged ORF) - Rat galanin receptor 2 (Galr2), 200 uL, >10^7 TU/mL |
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|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||