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GAPDH2 antibody

Principal name

GAPDH2 antibody

Alternative names for GAPDH2 antibody

GAPDHS, GAPD2, GAPDS, HSD35, HSD-35, Glyceraldehyde-3-phosphate dehydrogenase testis-specific, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2

SwissProt ID

O14556 (Human), Q2KJE5 (Bovin), Q64467 (Mouse)

Gene ID

26330 (GAPDHS)

Ncbi ID

NP_055179.1, 9606, NP_055179, NM_014364

Available reactivities

Hu (Human), Por (Porcine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of GAPDH2 antibody

Kit Component:: - KN205362G1, GAPDHS gRNA vector 1 in pCas-Guide vector
- KN205362G2, GAPDHS gRNA vector 2 in pCas-Guide vector
- KN205362D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

1. Tepla O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).
2. Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J.: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32. Czech.
3. Peknicova J, Kyselova V, Buckiova D, Boubelik M.: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.

Immunoperoxidase of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]

H00026330-M01

Western Blot analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.

H00026330-M01

Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.

H00026330-M01

Western Blot analysis of GAPDHS expression in transfected 293T cell line (H00026330-T02) by GAPDHS MaxPab polyclonal antibody.Lane 1: GAPDHS transfected lysate(44.50 KDa).Lane 2: Non-transfected lysate.

H00026330-D01P

Immunoprecipitation of GAPDHS transfected lysate using anti-GAPDHS MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GAPDHS MaxPab mouse polyclonal antibody (B01) (H00026330-B01).

H00026330-D01

Human Spleen; WB Suggested Anti-GAPDHS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; GAPDHS antibody - N-terminal region (ARP40187_P050) in Human Spleen cells using Western Blot

ARP40187_P050

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAPDHS (RC205362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAPDHS.

TA505454

TP305362

WB Suggested Anti-GAPDHS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Spleen

TA345676

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA505454	GAPDH2 antibody
	GAPDHS mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)
	Mouse	IgG2a	OTI1F6	Purified	Hu	P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+5 additional images	Highly validated antibody!
TA350930	GAPDH2 antibody
	Rabbit Polyclonal Anti-GAPDHS Antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
SM3115P	GAPDH2 antibody
	Mouse	IgM	Hs-8	Purified	Hu, Por	F, ICC/IF, WB	0.1 mg / €240.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP305362	GAPDH2
	Recombinant protein of human glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY402320	GAPDHS overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

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