GAS1 antibody
Principal name
GAS1 antibody
Alternative names for GAS1 antibody
Growth arrest-specific protein 1, GAS-1
Ncbi ID
NP_002039, NP_002039.2, NM_002048, NP_032112.1, BC058628, NM_008086
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of GAS1 antibody
- Kit Component:
- - KN306312G1, Gas1 gRNA vector 1 in pCas-Guide vector
- KN306312G2, Gas1 gRNA vector 2 in pCas-Guide vector
- KN306312D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ribeiro, L.A., et al. Am. J. Med. Genet. A 152A (7), 1688-1694 (2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010)
Rittie, L., et al. Aging Cell 8(6):738-751(2009)
Zhao, L., et al. J. Biol. Chem. 284(39):26273-26285(2009)
Dominguez-Monzon, G., et al. Int. J. Dev. Neurosci. 27(4):305-313(2009)
Western blot (WB) analysis of Gas1 antibody (Cat.-No.: AP21186PU-N) in extracts from MCF-7 cells.
Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue reacted with GAS1 Antibody (N-term) Cat.-NO AP51781PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Confocal immunofluorescent analysis of GAS1 Antibody (N-term) Cat.-No AP51781PU-N with NCI-H460 cell followed by Alexa Fluor®488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of GAS1 Antibody (N-term) Cat.-No AP51781PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the GAS1 antibody detected the GAS1 protein (arrow).
Flow cytometric analysis of NCI-H460 cells using GAS1 Antibiody (N-term) Cat.-No AP51781PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts from MCF-7 cells, using GAS1 antibody.The lane on the right is treated with the synthesized peptide.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, human kidney tissue, Primary antibody: (GAS1 Antibody) at dilution 1/100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (GAS1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP51781PU-N | GAS1 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
||||||
| TA313912 | GAS1 antibody |
||||||
| Rabbit polyclonal anti-GAS1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351209 | GAS1 antibody |
||||||
| Rabbit Polyclonal Anti-GAS1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP21186PU-N | GAS1 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN224804 | GAS1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN224804BN | GAS1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN224804LP | GAS1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN224804RB | GAS1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| PR15082-50 | GAS1 |
||||
|
Human | Purified | > 95 % | Mouse |
50 µg /
€410.00
|
| Neuromics Antibodies | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419569 | GAS1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205163L2V | Lenti ORF particles, Gas1 (GFP-tagged) - Mouse growth arrest specific 1 (cDNA clone MGC:73421 IMAGE:6401957), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR217242L2V | Lenti ORF particles, Gas1 (GFP-tagged) - Mouse growth arrest specific 1 (Gas1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205163L1V | Lenti ORF particles, Gas1 (Myc-DDK-tagged) - Mouse growth arrest specific 1 (cDNA clone MGC:73421 IMAGE:6401957), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR217242L1V | Lenti ORF particles, Gas1 (Myc-DDK-tagged) - Mouse growth arrest specific 1 (Gas1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
