GATA3 antibody
Principal name
GATA3 antibody
Alternative names for GATA3 antibody
GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
Ncbi ID
NP_001002295.1, NP_001002295, NP_002042, 9606, NM_002051, NM_001002295, NP_032117.1, NM_008091
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))
Background of GATA3 antibody
GATA3: GATA binding protein 3. The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells, macrophages, or HeLa cell lines. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1,but no other structural homologies between the 2 genes could be found.
General readings
Cook, K.D., et al. J. Immunol. 185(6):3209-3216(2010)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Kemp, K.L., et al. J. Immunol. 184(8):4178-4184(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Yao, X., et al. Chin. Med. J. 123(6):739-744(2010)
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Staining (3µg/ml) of MOLT-4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by chemiluminescence.
Western blot analysis of GATA3 (arrow) using GATA3 Antibody (Center) Cat.-No AP51785PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GATA3 gene.
Western blot analysis of GATA3 Antibody (Center) Cat.-No AP51785PU-N in mouse liver tissue lysates (35ug/lane). This demonstrates the GATA3 antibody detected the GATA3 protein (arrow).
Human kidney; Immunohistochemistry with Human kidney lysate tissue at an antibody concentration of 5.0ug/ml using anti-GATA3 antibody (ARP31857_P050); GATA3 antibody - N-terminal region (ARP31857_P050) in Human kidney cells using Immunohistochemistry
Hum. Fetal Heart; Host: Rabbit . Target Name: GATA3 . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; GATA3 antibody - N-terminal region (ARP31857_P050) in Hum. Fetal Heart cells using Western Blot
Hum. Fetal Lung; Host: Rabbit . Target Name: GATA3 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; GATA3 antibody - N-terminal region (ARP31857_P050) in Hum. Fetal Lung cells using Western Blot
MCF7; Host: Rabbit. Target Name:GATA3. Sample Tissue:MCF7. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5.0 ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 12%.; GATA3 antibody - N-terminal region (ARP31857_P050) in MCF7 cells using Western Blot
Human MCF-7; WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate; GATA3 antibody - N-terminal region (ARP31857_P050) in Human MCF-7 cells using Western Blot
Human MCF7; Host: Rabbit . Target Name: GATA3 . Sample Tissue: MCF7 . Antibody Dilution: 1.0ug/ml. GATA3 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; GATA3 antibody - N-terminal region (ARP31857_P050) in Human MCF7 cells using Western Blot
Human Liver; Rabbit Anti-GATA3 Antibody Catalog Number: ARP32548 Paraffin Embedded Tissue: Human Liver Cellular Data: Hepatocyte Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X; GATA3 antibody - C-terminal region (ARP32548_P050) in Human Liver cells using Immunohistochemistry
Human ESC; human embryonic stem cells. Dilition:1-250; GATA3 antibody - C-terminal region (ARP32548_T100) in Human ESC cells using Immunofluorescence
Human Jurkat; WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that GATA3 is expressed in Jurkat.; GATA3 antibody - C-terminal region (ARP32548_P050) in Human Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that GATA3 is expressed in Jurkat.; GATA3 antibody - C-terminal region (ARP32548_P050) in Human Jurkat cells using Western Blot
Human Liver; Rabbit Anti-GATA3 Antibody. Catalog Number: ARP32548. Paraffin Embedded Tissue: Human Liver. Cellular Data: Hepatocyte. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; GATA3 antibody - C-terminal region (ARP32548_T100) in Human Liver cells using Immunohistochemistry
Human Jurkat; WB Suggested Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat. There is BioGPS gene expression data showing that GATA3 is expressed in Jurkat.; GATA3 antibody - C-terminal region (ARP32548_T100) in Human Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that GATA3 is expressed in Jurkat.; GATA3 antibody - C-terminal region (ARP32548_T100) in Human Jurkat cells using Western Blot
Western Blot analysis of GATA3 expression in transfected 293T cell line (H00002625-T01) by GATA3 MaxPab polyclonal antibody.Lane 1: GATA3 transfected lysate(48.84 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GATA3 expression in transfected 293T cell line (H00002625-T01) by GATA3 MaxPab polyclonal antibody.Lane 1: GATA3 transfected lysate(48.84 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GATA3 expression in transfected 293T cell line (H00002625-T01) by GATA3 MaxPab polyclonal antibody.Lane 1: GATA3 transfected lysate(48.00 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GATA3 expression in transfected 293T cell line by GATA3 monoclonal antibody (M01), clone 1C1.Lane 1: GATA3 transfected lysate(48 KDa).Lane 2: Non-transfected lysate.
GATA3 monoclonal antibody (M01), clone 1C1 Western Blot analysis of GATA3 expression in A-431 ( Cat # L015V1 ).
GATA3 monoclonal antibody (M02), clone 2H5 Western Blot analysis of GATA3 expression in A-431 ( Cat # L015V1 ).
GATA3 monoclonal antibody (M09), clone 3A4. Western Blot analysis of GATA3 expression in Hela NE ( Cat # L013V3 ).
GATA3 monoclonal antibody (M09), clone 3A4. Western Blot analysis of GATA3 expression in Jurkat ( Cat # L017V1 ).
Detection limit for recombinant GST tagged GATA3 is approximately 0.03ng/ml as a capture antibody.
Western blot analysis using GATA3 antibody Cat.-No AM06216SU-N against truncated GATA3-His recombinant protein (Lane 1).
Immunohistochemical analysis of paraffin-embedded human Small Intestine, muscularis propria tissues using anti-GATA3 mouse mAb
Western blot analysis using GATA3 mouse mAb against GATA3(AA: 175-388)-hIgGFc transfected HEK293 cell lysate.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809094)(1:200)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GATA3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809094)(1:200)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809094)(1:200)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GATA3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809094)(1:200)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GATA3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GATA3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GATA3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GATA3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GATA3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:20
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:500)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809219)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809219)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809219)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809219)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000)
TA305795 (1.0µg/ml) staining of Molt4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA305795 (3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) staining of cells in the germinal centre, B) staining of cells in the interfollicular area.
Figure from citation: Immunohistochemistry of GATA3 protein level by using anti-GATA3 antibody in mouse lung sections. Dilution: 1:100–1:200 View Citation
Western blot analysis of GATA3 in human brain tissue lysate with GATA3 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry of GATA3 in rat brain tissue with GATA3 antibody at 2.5 ug/mL.
Immunofluorescence of GATA3 in Rat Brain tissue with GATA3 antibody at 20 ug/mL.
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using GATA3 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using GATA3 antibody.The picture on the right is treated with the synthesized peptide.
Western blot using 2 ug/mL Anti-Human/Mouse Gata-3 Purified) and revealed with Anti-Rat IgG HRP. Lane 1: 3 day anti-CD3/CD28 stimulated human CD4 cells, 2: 6 day Th2 polarized cultured human cells, 3: Jurkat cell line, 4: MCF7 cell line
Western blot analysis of GATA3 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that GATA3 is expressed in Jurkat
WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that GATA3 is expressed in Jurkat
Rabbit Anti-GATA3 Antibody Paraffin Embedded Tissue: Human Liver Cellular Data: Hepatocyte Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X
Sample Type : Embronic Stem cell H1 to HNF4 cell line;
WB Suggested Antibody Titration: 0.2-1 ug/ml; Positive Control: JurkatThere is BioGPS gene expression data showing that GATA3 is expressed in Jurkat
WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that GATA3 is expressed in Jurkat
Rabbit Anti-GATA3 Antibody; Paraffin Embedded Tissue: Human Liver; Cellular Data: Hepatocyte; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
human embryonic stem cells; Dilition:1-250
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN201618RB | GATA3 - human gene knockout kit via CRISPR |
||
 |
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
