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GATA4 antibody

Principal name

GATA4 antibody

Alternative names for GATA4 antibody

GATA-4, GATA-binding factor 4, Transcription factor GATA-4

SwissProt ID

P43691 (Chick), P43694 (Human), P46152 (Rat), Q08369 (Mouse), Q0Q0E4 (Canfa), Q91677 (Xenla)

Gene ID

2626 (GATA4), 14463 (Gata4), 54254

Ncbi ID

NP_002043, NP_002043.2, 9606, NP_032118.2, NM_008092, NM_144730, NM_002052

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Chk (Chicken)

Available hosts

Rabbit, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GATA4 antibody

The eBioEvan monoclol antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The eBioEvan monoclol antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4. Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved D binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gods.

General readings

1. Elabd C et al. DNA methyltransferase-3-dependent nonrandom template segregation in differentiating embryonic stem cells. J Cell Biol 203:73-85 (2013).
2. He Z et al. Transduction of Wnt11 promotes mesenchymal stem cell transdifferentiation into cardiac phenotypes. Stem Cells Dev 20:1771-8 (2011). WB ; Rat .
3. Mamidi MK et al. Application of multiplex PCR for characterization of human embryonic stem cells (hESCs) and its differentiated progenies. J Biomol Screen 15:630-43 (2010).
4. Wilson KD et al. Dynamic microRNA expression programs during cardiac differentiation of human embryonic stem cells: role for miR-499. Circ Cardiovasc Genet 3:426-35 (2010). WB; Human .
5. Li H et al. Paracrine factors released by GATA-4 overexpressed mesenchymal stem cells increase angiogenesis and cell survival. Am J Physiol Heart Circ Physiol 299:H1772-81 (2010). ICC/IF ; Rat .
6. Chen S et al. Transcriptional Co-activator p300 Regulates Glucose Induced Gene Expression in the Endothelial Cells. Am J Physiol Endocrinol Metab : (2009). WB ; Human .

Mouse tumor; . Lanes: 1: 25 ug mouse tumor + shRNA1, 2: 25 ug mouse tumor + shRNA2, 3: 25 ug mouse tumor + shRNA3 4: 25 ug mouse tumor lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:3500. Gene Name: GATA4. Submitted by: Dr. Jonahan Kurie, UT MD Anderson Cancer center.; GATA4 antibody - N-terminal region (ARP38100_P050) in Mouse tumor cells using Western Blot

ARP38100_P050

Human Jurkat; WB Suggested Anti-GATA4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; GATA4 antibody - N-terminal region (ARP38100_P050) in Human Jurkat cells using Western Blot

ARP38100_P050

Immunohistochemistry (IHC) analyzes of GATA-4 antibody (Cat.-No.: AP06693PU-N) in paraffin-embedded human heart tissue.

AP06693PU-N

Western blot (WB) analysis of GATA4 antibody (Cat.-No.: AP06693PU-N) at 1/500 dilution
Lane1:HepG2 whole cell lysate
Lane2:Mouse liver tissue lysate
Lane3:Rat liver tissue lysate
Lane4:Rat kidney tissue lysate

AP06693PU-N

Western blot analysis of GATA4 ( arrow ) using rabbit GATA4 polyclonal antibody ( Cat # PAB2480 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the GATA4 gene ( Lane 2 ) ( Origene Technologies ) .

PAB2480

Western blot analysis of GATA4 polyclonal antibody ( Cat # PAB2480 ) in CEM cell line lysates ( 35 µg/lane ) . GATA4 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .

PAB2480

Western blot analysis of GATA4 antibody (AP20231PU-N) in extracts from COLO205 cells.

AP20231PU-N

Immunohistochemistry analyzes of GATA4 antibody (Cat.-No.: AP20302PU-N) in paraffin-embedded human lung carcinoma tissue.

AP20302PU-N

Western blot analysis of GATA4 antibody (Cat.-No.: AP20302PU-N) in extracts from HeLa cells.

AP20302PU-N

Immunofluorescence analysis of HuvEc cells, using GATA4 (Phospho-Ser262) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells, using GATA4 (Phospho-Ser262) Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HepG2 cells, using GATA4 (Phospho-Ser105) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using GATA4 (Ab-262) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COLO205 cells, treated with Serum 20% 15', using GATA4 (Ab-262) Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using GATA4 (Ab-105) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, using GATA4 (Ab-105) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, using GATA4 Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis using GATA4 antibody Cat.-No AM06352SU-N against rat fetal heart (Lane 1) and adult heart (Lane 2) tissues lysate.

AM06352SU-N

Immunohistochemical analysis of GATA4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of GATA4 expression in HepG2 (A); NIH3T3 (B); mouse liver (C); rat liver (D); rat kidney (E) whole cell lysates.

Immunohistochemical analysis of GATA4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GATA4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of GATA4 expression in HepG2 (A); HeLa (B) whole cell lysates.

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GATA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500091)

TA500091

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GATA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500091)

TA500091

Anti-GATA4 mouse monoclonal antibody (TA500091) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA4(RC210945).

TA500091

Immunofluorescent staining of HeLa cells using anti-GATA4 mouse monoclonal antibody (TA500091).

TA500091

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GATA4 monoclonal antibody.

TA500091

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA4 (RC210945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA4.

TA500091

Anti-GATA4 mouse monoclonal antibody (TA500253) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA4(RC210945).

TA500253

TA500253

Figure from citation: Western Blot of GATA4 protein level by using anti-GATA4 antibody in hESCs. View Citation

TA500253

TP310945

Western blot analysis of anti-GATA4 Antibody (C-term) (Cat.#TA302164) in CEM cell line lysates (35ug/lane). GATA4(arrow) was detected using the purified Pab.

TA302164

Western blot analysis of GATA4 (arrow) using rabbit polyclonal GATA4 Antibody (C-term) (Cat.#TA302164). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2) (Origene Technologies).

TA302164

GATA4 Antibody (C-term) (Cat. #TA302164) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TA302164

Anti-Human/Mouse Gata-4 Purified (2 ug/ml) was used in a western blot of SDS lysates. Lane 1: NIH-3T3, Lane 2: C57Bl/6 mouse heart, Lane 3: BALB/c mouse heart.

TA320339

Predicted cell location: Nucleus. Positive control: Human brain tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using GATA4 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification: x200)

TA323061

Western blot analysis of GATA4 expression in Serum treated COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325500

Western blot analysis of GATA4 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325501

Western blot analysis of extracts from Jurkat cells, using GATA4 antibody.The lane on the right is treated with the synthesized peptide.

TA326262

Western Blot: GATA4 Antibody (6H10) [TA336777] - Western blot analysis using anti-GATA4 mAb against rat fetal heart (1) and adult heart (2) tissues lysate.

TA336777

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA500253	GATA4 antibody
	anti-GATA4 mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)
	Mouse	IgG2b	OTI1H8	Purified	Hu	ICC/IF, WB	0.1 ml / €360.00
							OriGene Technologies, Inc.
+2 additional images	Published recently!
TA500091	GATA4 antibody
	GATA4 mouse monoclonal antibody, clone OTI9F9 (formerly 9F9)
	Mouse	IgG3	OTI9F9	Purified	Hu	ICC/IF, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+5 additional images	Highly validated antibody!
TA302164	GATA4 (C-term) antibody
	Rabbit Polyclonal Antibody against GATA4 (C-term)
	Rabbit	Ig		Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies, Inc.
+2 additional images
TA320339	GATA4 antibody
	Rat Anti-Human/Mouse Gata-4 Purified (25 ug)
	Rat		eBioEvan	Purified	Hu, Ms	P, WB	25 µg / €165.00
							OriGene Technologies, Inc.
TA323061	GATA4 antibody
	Anti-GATA4 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA325500	GATA4 antibody
	Rabbit Polyclonal GATA4 Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA325501	GATA4 antibody
	Rabbit Polyclonal GATA4 Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA326262	GATA4 antibody
	Rabbit polyclonal GATA4 antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA336777	GATA4 antibody
	Mouse Monoclonal GATA-4 Antibody (6H10)
	Mouse	IgG2b	6H10	Purified	Hu, Ms, Rt	E, WB	0.1 ml / €395.00
							OriGene Technologies, Inc.
AM06352SU-N	GATA4 antibody
	Mouse	IgG2b	6H10	Ascites	Hu, Ms	E, WB	0.1 ml / €420.00
							OriGene Technologies GmbH
AP06693PU-N	GATA4 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image
AP20231PU-N	GATA4 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
AP20302PU-N	GATA4 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No.	Species	Donor Vector
KN210945	GATA4 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN210945BN	GATA4 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN210945LP	GATA4 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN210945RB	GATA4 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP310945	GATA4
	Recombinant protein of human GATA binding protein 4 (GATA4)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR204299L2V	Lenti ORF particles, Gata4 (GFP-tagged ORF) - Rat GATA binding protein 4 (Gata4), 200 uL, >10^7 TU/mL
		200 µl / €1,100.00
		OriGene Technologies, Inc.

RR204299L1V	Lenti ORF particles, Gata4 (Myc-DDK-tagged ORF) - Rat GATA binding protein 4 (Gata4), 200 uL, >10^7 TU/mL
		200 µl / €990.00
		OriGene Technologies, Inc.

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