GBE1 antibody
Principal name
GBE1 antibody
Alternative names for GBE1 antibody
Glycogen-branching enzyme, Brancher enzyme, 1,4-alpha-glucan-branching enzyme
Ncbi ID
9606, NP_000149, NP_000149.3, NP_083079.1, NM_028803, NM_000158
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)
Background of GBE1 antibody
- Kit Component:
- - KN306337G1, Gbe1 gRNA vector 1 in pCas-Guide vector
- KN306337G2, Gbe1 gRNA vector 2 in pCas-Guide vector
- KN306337D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Konstantinidou, A.E., et al. Placenta 29(4):378-381(2008)
Massa, R., et al. Muscle Nerve 37(4):530-536(2008)
Bruno, C., et al. Acta Myol 26(1):75-78(2007)
GBE1 MaxPab polyclonal antibody. Western Blot analysis of GBE1 expression in human liver.
Western Blot analysis of GBE1 expression in transfected 293T cell line (H00002632-T01) by GBE1 MaxPab polyclonal antibody.Lane 1: GBE1 transfected lysate(77.22 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GBE1 expression in transfected 293T cell line (H00002632-T01) by GBE1 MaxPab polyclonal antibody.Lane 1: GBE1 transfected lysate(80.40 KDa).Lane 2: Non-transfected lysate.
Confocal immunofluorescent analysis of GBE1 Antibody (Center) Cat.-No AP51790PU-N with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of GBE1 Antibody (Center) Cat.-No AP51790PU-N in mouse liver tissue lysates (35ug/lane).
Western blot analysis of GBE1 Antibody (Center) Cat.-No AP51790PU-N in HL-60 cell line lysates (35ug/lane).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500779)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500779)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500779)
Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500801)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500801)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500801)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500801)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500801)
Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-GBE1 monoclonal antibody.(1:200)
Figure from citation: Western Blot of GBE1 protein level by using anti-GBE1 antibody in mouse muscle extracts obtained from Gbe1-/-. Gbe1+/- and Gbe1+/+ embryos. View Citation
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500802)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500802)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500802)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500802)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC204152 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GBE1 antibody(TA500802), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-GBE1 antibody(TA500802),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-GBE1 antibody(TA500802),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500803)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500803)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-GBE1 monoclonal antibody.(1:200)
Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Anti-GBE1 mouse monoclonal antibody (TA500807) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1(RC204152).
Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
Flow cytometric Analysis of Hela cells, using anti-GBE1 antibody(TA500807),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500829)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500829)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500829)
Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody (TA500829).
Anti-GBE1 mouse monoclonal antibody (TA500829) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1(RC204152).
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-GBE1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Figure from citation: Western Blot of GBE protein level by using anti-GBE antibody in mouse muscle extracts obtained from Gbe1-/-. Gbe1+/- and Gbe1+/+ embryos. View Citation
Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-GBE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
HEK293T cells transfected with either RC204152 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GBE1 antibody(TA500829), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-GBE1 antibody(TA500829),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500846)
Anti-GBE1 mouse monoclonal antibody (TA500846) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1(RC204152).
Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody (TA500846).
Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
Flow cytometric Analysis of Hela cells, using anti-GBE1 antibody(TA500846),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-GBE1 antibody(TA500846),(Red), compared to a nonspecific negative control antibody,(Blue).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP51790PU-N | GBE1 (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
 +2 additional images |
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