Gelsolin antibody
Principal name
Gelsolin antibody
Alternative names for Gelsolin antibody
Brevin, AGEL, GSN, Actin-depolymerizing factor
SwissProt ID
O93510 (Chick), P06396 (Human), P13020 (Mouse), P20305 (Pig), Q28372 (Horse), Q3SX14 (Bovin), Q68FP1 (Rat)
Ncbi ID
NP_000168.1, 9606, NP_000168, NM_000177, NM_146120, NM_001004080, NM_198252, NM_001127662, NM_001127663, NM_001127664, NM_001127665, NM_001127667, NM_001127666, NP_666232.2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Gelsolin antibody
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
General readings
Shokouhi,G. Nephrol. Dial. Transplant. 23 (3), 1071 (2008)
Immunoperoxidase of monoclonal antibody to GSN on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 ug/ml]
GSN monoclonal antibody (M01), clone 3G5 Western Blot analysis of GSN expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged GSN is approximately 0.03ng/ml as a capture antibody.
GSN polyclonal antibody ( Cat # PAB7148 ) ( 0.1 µg/ml ) staining of human spleen lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoblot analysis of human plasma protein and cell lysates. Lane 1 : Gelsolin isolated from human plasma. Lane 2 : Human plasma. Lane 3 : NIH : OVCAR-3 cell lysate. Lane 4 : MCF-7 cell lysate.
GSN MaxPab polyclonal antibody. Western Blot analysis of GSN expression in human stomach.
Western Blot analysis of GSN expression in transfected 293T cell line (H00002934-T01) by GSN MaxPab polyclonal antibody.Lane 1: GSN transfected lysate(86.02 KDa).Lane 2: Non-transfected lysate.
GSN MaxPab polyclonal antibody. Western Blot analysis of GSN expression in HepG2.
GSN MaxPab polyclonal antibody. Western Blot analysis of GSN expression in human stomach.
Western Blot analysis of GSN expression in transfected 293T cell line (H00002934-T01) by GSN MaxPab polyclonal antibody.Lane 1: GSN transfected lysate(86.02 KDa).Lane 2: Non-transfected lysate.
GSN MaxPab polyclonal antibody. Western Blot analysis of GSN expression in HepG2.
GSN MaxPab rabbit polyclonal antibody. Western Blot analysis of GSN expression in human liver.
Western Blot analysis of GSN expression in transfected 293T cell line (H00002934-T01) by GSN MaxPab polyclonal antibody.Lane 1: GSN transfected lysate(85.70 KDa).Lane 2: Non-transfected lysate.
GSN MaxPab rabbit polyclonal antibody. Western Blot analysis of GSN expression in HepG2.
Human Tonsil; . IHC Information: Paraffin embedded tonsil tissue, tested with an antibody Dilution of 5 ug/ml.; GSN antibody - C-terminal region (ARP54299_P050) in Human Tonsil cells using Immunohistochemistry
Human Tonsil; GSN antibody - C-terminal region (ARP54299_P050) validated by WB using pFA fixed human tonsil tissue; GSN antibody - C-terminal region (ARP54299_P050) in Human Tonsil cells using Western Blot
Human MCF7; WB Suggested Anti-GSN Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; GSN antibody - C-terminal region (ARP54299_P050) in Human MCF7 cells using Western Blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of methanol-fixed HeLa, using Gelsolin antibody at 1/200 dilution.
Sample (30 µg of whole cell lysate). A: H1299. B: Hela. 7.5% SDS PAGE. GSN antibody diluted at 1/1000.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
GSN monoclonal antibody (M01), clone 3G5 Western Blot analysis of GSN expression in HeLa.
Detection limit for recombinant GST tagged GSN is approximately 0.03ng/ml as a capture antibody.
AP31939PU-N Gelsolin antibody staining of Human Duodenum lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA303164 (0.1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry of paraffin-embedded Human brain tissue using TA323717(GSN Antibody)(left image) at dilution 1/40, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA323717 (GSN Antibody)(left image) at dilution 1/40, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA323718 (GSN Antibody)(left image) at dilution 1/40, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323718 (GSN Antibody)(left image) at dilution 1/40, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Western blot analysis of extracts of RAW264.7 cell lysate, using GSN antibody.
Immunohistochemistry of paraffin-embedded human ovarian cancer tissue using GSN antibody.
WB Suggested Anti-GSN Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate
GSN antibody - C-terminal region validated by WB using pFA fixed human tonsil tissue
IHC Information: Paraffin embedded tonsil tissue, tested with an antibody Dilution of 5 ug/ml.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP31939PU-N | Gelsolin (Isoform a) antibody |
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Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA303164 | Gelsolin antibody |
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| Goat Polyclonal Antibody against GSN | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA323717 | Gelsolin antibody |
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| Anti-GSN Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA323718 | Gelsolin antibody |
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| Anti-GSN Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA327454 | Gelsolin antibody |
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| GSN Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM20623PU-N | Gelsolin antibody |
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 |
Mouse | IgG1 | GEL-42 | Purified | Hu, Rb | C, P, WB |
0.1 mg /
€390.00
|
| OriGene Technologies GmbH | |||||||
| AM31077PU-N | Gelsolin antibody |
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 |
Mouse | IgG1 | 3G5 | Purified | Hu | E, IP, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP23589PU-N | Gelsolin (161-369) antibody |
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 |
Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
50 µl /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP314871 | Gelsolin (transcript variant 1) |
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| Recombinant protein of human gelsolin (amyloidosis, Finnish type) (GSN), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY424875 | GSN overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC206861L2V | Lenti ORF particles, GSN (mGFP-tagged)-Human gelsolin (GSN), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201675L2V | Lenti ORF particles, Gsn (GFP-tagged ORF) - Rat gelsolin (Gsn), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,580.00
|
|
| OriGene Technologies, Inc. | ||
| RR201675L1V | Lenti ORF particles, Gsn (Myc-DDK-tagged ORF) - Rat gelsolin (Gsn), 200 uL, >10^7 TU/mL |
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200 µl /
€1,420.00
|
|
| OriGene Technologies, Inc. | ||
