Gephyrin antibody
Principal name
Gephyrin antibody
Alternative names for Gephyrin antibody
GPHN, GPH, KIAA1385
Ncbi ID
9606, NP_001019389, ABM86601.1, NP_001019389.1, NP_065857, NM_172952, NM_145965, NM_022865, NM_001024218
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Gephyrin antibody
GPHN is a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known.
General readings
Fritschy,J.M., (er) Trends Neurosci. (2008) In press
GPHN polyclonal antibody (A01), Lot # 060118JC01 Western Blot analysis of GPHN expression in Jurkat ( Cat # L017V1 ).
Human Jurkat; WB Suggested Anti-GPHN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; GPHN antibody - middle region (ARP51839_P050) in Human Jurkat cells using Western Blot
Human 721_B; WB Suggested Anti-GPHN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate. GPHN is supported by BioGPS gene expression data to be expressed in 721_B.; GPHN antibody - N-terminal region (ARP51838_P050) in Human 721_B cells using Western Blot
GPHN Antibody (Center) (Cat. #AP51909PU-N) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPHN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
GPHN Antibody (Center) (Cat. #AP51909PU-N) western blot analysis in MCF-7,Hela,NCI-H460,HepG2 cell line and mouse liver tissue lysates (35µg/lane).This demonstrates the GPHN antibody detected the GPHN protein (arrow).
GPHN Antibody (Center) (Cat. #AP51909PU-N) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-GPHN mouse monoclonal antibody (TA502216) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPHN(RC205986).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody.
HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502216), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502217)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502217)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502217)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502217)
Anti-GPHN mouse monoclonal antibody (TA502217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPHN(RC205986).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502217), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-GPHN antibody(TA502217),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-GPHN mouse monoclonal antibody (TA502218) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPHN(RC205986).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
Flow cytometric Analysis of Hela cells, using anti-GPHN antibody(TA502247),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502247), and then analyzed by flow cytometry.
Anti-GPHN mouse monoclonal antibody (TA502248) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPHN(RC205986).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502248), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502283), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502284), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
Flow cytometric Analysis of Hela cells, using anti-GPHN antibody(TA502312),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502312), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502313), and then analyzed by flow cytometry.
WB Suggested Anti-GPHN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 721_B cell lysate GPHN is supported by BioGPS gene expression data to be expressed in 721_B
WB Suggested Anti-GPHN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (GPHN Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using (GPHN Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502216 | Gephyrin antibody |
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| GPHN (Gephyrin) mouse monoclonal antibody, clone OTI2C2 (formerly 2C2) | |||||||
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Mouse | IgG2a | OTI2C2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA502217 | Gephyrin antibody |
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| GPHN (Gephyrin) mouse monoclonal antibody, clone OTI3B6 (formerly 3B6) | |||||||
 |
Mouse | IgG2a | OTI3B6 | Purified | Hu, Mky | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA502247 | Gephyrin antibody |
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| GPHN (Gephyrin) mouse monoclonal antibody, clone OTI3C6 (formerly 3C6) | |||||||
 |
Mouse | IgG2a | OTI3C6 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
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| TA502312 | Gephyrin antibody |
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| GPHN (Gephyrin) mouse monoclonal antibody, clone OTI3A6 (formerly 3A6) | |||||||
 |
Mouse | IgG2a | OTI3A6 | Purified | Hu, Mky, Rt | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA351213 | Gephyrin antibody |
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| Rabbit Polyclonal Anti-GPHN Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA502218 | Gephyrin antibody |
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| GPHN (Gephyrin) mouse monoclonal antibody, clone OTI1C5 (formerly 1C5) | |||||||
 |
Mouse | IgG2a | OTI1C5 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA502248 | Gephyrin antibody |
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| GPHN (Gephyrin) mouse monoclonal antibody, clone OTI5B6 (formerly 5B6) | |||||||
 |
Mouse | IgG2a | OTI5B6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA502283 | Gephyrin antibody |
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| GPHN (Gephyrin) mouse monoclonal antibody, clone OTI7C6 (formerly 7C6) | |||||||
 |
Mouse | IgG2a | OTI7C6 | Purified | Hu, Mky | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA502284 | Gephyrin antibody |
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| GPHN (Gephyrin) mouse monoclonal antibody, clone OTI7B6 (formerly 7B6) | |||||||
 |
Mouse | IgG2a | OTI7B6 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA502313 | Gephyrin antibody |
||||||
| GPHN (Gephyrin) mouse monoclonal antibody, clone OTI5C9 (formerly 5C9) | |||||||
 |
Mouse | IgG2a | OTI5C9 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP51909PU-N | Gephyrin (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu, Ms | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305986 | Gephyrin (transcript variant 1) |
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| Recombinant protein of human gephyrin (GPHN), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213663L2V | Lenti ORF particles, Gphn (GFP-tagged ORF) - Rat gephyrin (Gphn), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,520.00
|
|
| OriGene Technologies, Inc. | ||
| RR213663L1V | Lenti ORF particles, Gphn (Myc-DDK-tagged ORF) - Rat gephyrin (Gphn), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,360.00
|
|
| OriGene Technologies, Inc. | ||
