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GGA3 antibody

Principal name

GGA3 antibody

Alternative names for GGA3 antibody

KIAA0154, ADP-ribosylation factor-binding protein GGA3, Golgi-localized gamma ear-containing ARF-binding protein 3

SwissProt ID

Q8BMI3 (Mouse), Q9NZ52 (Human)

Gene ID

23163 (GGA3)

Ncbi ID

9606, NP_001166174.1, NP_619525, NP_054720, NM_001172704, NM_001172703, NM_014001, NM_138619

Available reactivities

Hu (Human), Macaque, Mky (Monkey), Ms (Mouse), Rt (Rat), Bt (Bat), Can (Canine), Chk (Chicken), Eq (Equine), Hst (Hamster), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), Bear, Chimp (Chimpanzee), Orang-Utan, Bov (Bovine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of GGA3 antibody

GGA3 (also known as ADP-ribosylation factor binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, Gamma-adaptin related protein 3) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200, GGA1 and GGA2 as well as RABEP1, RABGEF1 and type-I membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA3 is a ubiquitously expressed membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Breast.

AP07588PU-N

Western Blot analysis of GGA3 expression in transfected 293T cell line (H00023163-T01) by GGA3 MaxPab polyclonal antibody.Lane 1: GGA3 transfected lysate(74.50 KDa).Lane 2: Non-transfected lysate.

H00023163-B01

Western blot using GGA3 polyclonal antibody ( Cat # PAB10050 ) shows detection of aband at ~110 kDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cellsover- expressing the recombinant protein (lane 2, arrowhead ).Pre-incubation of antibody with immunizing peptide blocks specific staining ( lane3 ).MW markers are shown in lane 1 ( 700 nm channel, red ).Approximately 35 µg of lysate was separated on a 16% Tricine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1:600.Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye^TM800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature ( 800 nm channel, green ).IRDye^TM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.IRDye is a trademark of LI-COR, Inc.

PAB10050

Human Thymus; WB Suggested Anti-GGA3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Thymus; GGA3 antibody - N-terminal region (ARP55423_P050) in Human Thymus cells using Western Blot

ARP55423_P050

Human Kidney (formalin-fixed, paraffin-embedded) stained with GGA3 antibody AP22476PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22476PU-N

TP313361

WB using Anti-GGA3 antibody shows detection of a band at ~110 kDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cells over- expressing the recombinant protein (lane 2, arrowhead). Pre-incubation of antibody with immunizing peptide blocks specific staining (lane 3). MW markers are shown in lane 1 (700 nm channel - red).The primary antibody was diluted to 1:600.

TA319379

WB Suggested Anti-GGA3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Thymus

TA333540

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA319379	GGA3 (400-415) antibody
	Rabbit polyclonal anti-GGA3 antibody
	Rabbit	IgG	Aff - Purified	Chimp, Hu, Macaque, Orang-Utan	E, WB	0.1 mg / €515.00
						OriGene Technologies, Inc.
AP07588PU-N	GGA3 (400-415) antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, P, WB	50 µg / €425.00
						OriGene Technologies GmbH
AP22476PU-N	GGA3 (702-713) antibody
	Goat		Aff - Purified	Bt, Elephant, Eq, Hu, Mky, Por, Rb	E, P	50 µg / €425.00
						OriGene Technologies GmbH

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