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GLI3 antibody

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Principal name

GLI3 antibody

Alternative names for GLI3 antibody

GLI-3, Zinc finger protein GLI3

SwissProt ID

P10071 (Human), P55879 (Chick), Q61602 (Mouse)

Gene ID

2737 (GLI3), 14634 (Gli3)

Ncbi ID

NP_000159.3, 9606, NP_000159, NM_008130, NM_000168, NP_032156.2

Available reactivities

Can (Canine), Chk (Chicken), Frog, Hu (Human), Mky (Monkey), Quail, Rt (Rat), Ms (Mouse), Chimp (Chimpanzee), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of GLI3 antibody

GLI3(Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila.The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.

General readings

1. Barna, M., Pandolfi, P. P., Niswander, L. Gli3 and Plzf cooperate in proximal limb patterning at early
stages of limb development. (Letter) Nature 436: 277-281, 2005.
2. Bose, J., Grotewold, L., Ruther, U. Pallister-Hall syndrome phenotype in mice mutant for Gli3. Hum.
Molec. Genet. 11: 1129-1135, 2002.
3. Craig, D. W., Itty, A., Panganiban, C., Szelinger, S., Kruer, M. C., Sekar, A., Reiman, D., Narayanan,
V., Stephan, D. A., Kerrigan, J. F.Identification of somatic chromosomal abnormalities in
hypothalamic hamartoma tissue at the GLI3 locus. Am. J. Hum. Genet. 82: 366-374, 2008.

AP07869PU-N - GLI3
Figure 1. Staining GLI3 in Brain, Glioblastoma by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
C10328-1 - GLI3
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GLI-3 Antibody. The picture on the right is treated with the synthesized peptide.
C10328-1 - GLI3
Immunofluorescence analysis of HepG2 cells, using GLI-3 Antibody. The picture on the right is treated with the synthesized peptide.
H00002737-A01 - GLI3
GLI3 polyclonal antibody (A01), Lot # 051017JC01 Western Blot analysis of GLI3 expression in HL-60 ( Cat # L014V1 ).
H00002737-M01 - GLI3
Proximity Ligation Analysis (PLA) of protein-protein interactions between SMAD1 and GLI3. HeLa cells were stained with anti-SMAD1 rabbit purified polyclonal 1:1200 and anti-GLI3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00002737-M01 - GLI3
Detection limit for recombinant GST tagged GLI3 is approximately 1ng/ml as a capture antibody.
PAB10052 - GLI3
Immunohistochemistry of GLI3 polyclonal antibody ( Cat # PAB10052 ) was used at a 0.625 µg/ml to detect GLI3 in a variety of tissues.Strong nuclear and smooth muscle staining was noted to be consistent with previously published reports.Specific staining was noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus.This image shows GLI3 staining of human glioblastoma.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
PAB10052 - GLI3
Western blot using GLI3 polyclonal antibody ( Cat # PAB10052 ) shows detection of multiple bands in human lung lysate believed to be GLI3.Lanes contain 20 µg of whole cell lysates from 1 - human brain, 2 - human lung, 3 - human spleen, 4 - mouse brain and 5 - mouse lung.While no recognizable staining can be seen on mouse tissue, human lung shows what may be truncated GLI3 ( ~80kDa ).This identity of the strong band at ~50 kDa is unknown.After blocking, the membrane was probed with the primary antibody diluted to 1:500.For detection use HRP Gt-a-Rabbit IgG.Detection of GLI3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of GLI3 is likely to be low.Furthermore, GLI3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
TA313928 - GLI3
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GLI-3 antibody.The picture on the right is treated with the synthesized peptide.
TA313928 - GLI3
Immunofluorescence analysis of HepG2 cells, using GLI-3 antibody.The picture on the right is treated with the synthesized peptide.
TA319303 - GLI3
WB of Rabbit anti-Gli-3 antibody. Lane 1: human brain whole cell lysate. Lane 2: human lung whole cell lysate. Lane 3: human spleen whole cell lysate. Lane 4: mouse brain whole cell lysate. Lane 5: mouse lung whole cell lysate. Load: 20 µg per lane. Primary antibody: Gli-3 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
TA319303 - GLI3
IHC of Rabbit anti-Gli-3 antibody. This image tissue: human glioblastoma. Specific staining was also noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus. Primary antibody: Gli-3 antibody at 0.625 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
TA319303 - GLI3
Immunofluorescence Microscopy of Rabbit anti-Gli-3 antibody. Tissue: MCF-7 cell. Antigen retrieval: not required. Primary antibody: Gli-3 antibody and Anti-alpha-Tubulin at 5 µg/mL for 1 h at RT. Secondary antibody: Fluorescein secondary antibody at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear. Staining: Gli-3 staining as red fluorescent signal and Anti-alpha-Tubulin staining as green fluorescent signal using STED.
TA319303 - GLI3
IF of Rabbit anti-Gli-3 antibody. Tissue: MCF-7 cell. Antigen retrieval: not required. Primary antibody: Gli-3 antibody and Anti-alpha-Tubulin at 5 µg/mL for 1 h at RT. Secondary antibody: Fluorescein secondary antibody at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear. Staining: Image (1) shows alpha-Tubulin staining as green fluorescent signal. Image (2) shows Gli-3 staining as red fluorescent signal and Images (3) shows both antibodies fluorescing using STED microscopy.
TA337186 - GLI3
Western Blot: Gli3 Antibody [TA337186] - WB analysis of Gli3 in Hela cell lysate and MEF cell lysate.
TA337186 - GLI3
Immunohistochemistry-Paraffin: Gli3 Antibody [TA337186] - IHC analysis of Gli3 antibody in mouse liver.
TA337186 - GLI3
Immunohistochemistry-Paraffin: Gli3 Antibody [TA337186] - IHC analysis of Gli3 antibody in mouse testis.
TA337186 - GLI3
Immunocytochemistry/Immunofluorescence: Gli3 Antibody [TA337186] - Gli3 antibody was tested in Hela cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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  2. Highly Validated (2)

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GLI3 (41-57) antibody
  Rabbit polyclonal anti-Gli-3 antibody  
WB of Rabbit anti-Gli-3 antibody. Lane 1: human brain whole cell lysate. Lane 2: human lung whole cell lysate. Lane 3: human spleen whole cell lysate. Lane 4: mouse brain whole cell lysate. Lane 5: mouse lung whole cell lysate. Load: 20 µg per lane. Primary antibody: Gli-3 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Rabbit IgG Aff - Purified Can, Chimp, Chk, Hu, Mky, Ms, Rt, Xen E, P, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

GLI3 antibody
  Rabbit Polyclonal GLI-3 Antibody  
Western Blot: Gli3 Antibody [TA337186] - WB analysis of Gli3 in Hela cell lysate and MEF cell lysate. Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 mg / €335.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

GLI3 antibody
  Rabbit polyclonal anti-GLI-3 antibody  
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GLI-3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

GLI3 (41-57) antibody
Figure 1. Staining GLI3 in Brain, Glioblastoma by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Can, Chk, Hu, Mky, Quail, Xen E, ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

4 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (4)
Clonality
  1. Polyclonal (4)
Target
  1. GLI3 (4)
Property
  1. 41-57 (2)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (3)
  3. Canine (Can) (2)
  4. Chicken (Chk) (2)
  5. Monkey (Mky) (2)
  6. Xenopus (Xen) (2)
  7. Chimpanzee (Chimp) (1)
  8. Quail (1)
  9. Rat (Rt) (1)
Applications
  1. Paraffin Sections (P) (4)
  2. Enzyme Immunoassay (E) (3)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  4. Western blot / Immunoblot (WB) (3)
  5. Frozen sections (C) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (2)
Host
  1. Rabbit (4)
Images
  1. 1 image (4)
  2. 3+ images (2)
Images: Applications
  1. Immunofluorescence (IF) (3)
  2. IHC (3)
  3. Western blot / Immunoblot (WB) (2)
  4. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (4)
Service
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