Goat IgG antibody
Principal name
Goat IgG antibody
Alternative names for Goat IgG antibody
Goat Immunoglobulin G
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Immunodiffusion (ID)
General readings
J.A. Titus, P.P. Haugland, S.D. Sharrow, D.M. Segal J. Immunol. Methods 50; 193, 1982. (Conjugation)

Western Blot of Rabbit anti-Goat Antibody Alkaline Phosphatase Conjugated:
Lane 1: Goat IgG, Load: 100 ng per lane Secondary antibody Alkaline Phosphatase Conjugated Rabbit Anti-Goat Antibody at 1/1000 for 60 min at RT
Block: MB-070 30 min RT
Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa
Lane 1: Goat IgG, Load: 100 ng per lane Secondary antibody Alkaline Phosphatase Conjugated Rabbit Anti-Goat Antibody at 1/1000 for 60 min at RT
Block: MB-070 30 min RT
Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa

Western Blot of Rabbit anti-Goat IgG (H&L) Antibody Fluorescein Conjugated:
Lane 1: Goat IgG, Load: 50 ng per lane Secondary antibody: Goat IgG (H&L) Antibody Fluorescein Conjugated at 1/1,000 for 60 min at RT
Block: MB-070 for 30 min at RT
Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa
Lane 1: Goat IgG, Load: 50 ng per lane Secondary antibody: Goat IgG (H&L) Antibody Fluorescein Conjugated at 1/1,000 for 60 min at RT
Block: MB-070 for 30 min at RT
Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa

Western Blot of Rabbit anti-GOAT IgG (H&L) Antibody Peroxidase Conjugated:
Lane 1: Goat IgG, Load: 100 ng per lane Secondary antibody GOAT IgG (H&L) Antibody Peroxidase Conjugated at 1/1,000 for 60 min at RT
Block: MB-070 for 30 min at RT
Lane 1: Goat IgG, Load: 100 ng per lane Secondary antibody GOAT IgG (H&L) Antibody Peroxidase Conjugated at 1/1,000 for 60 min at RT
Block: MB-070 for 30 min at RT

Western Blot of HRP conjugated anti-Goat IgG antibody R1318HRP showing detection of 50ng of Goat IgG (Lane 1) but not Human IgG (Lane 2). Samples were separated by 4-20% SDS-PAGE under reducing conditions and transferred to nitrocellulose membrane. The blot was blocked overnight at 4°C in 5% BSA in TBS. A 1/5,000 dilution of antibody in Blocking Buffer for Fluorescent Western Blotting was used to probe the membrane at RT for 1 h. The image was developed using Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate (p/n Femtomax-020) for one minute.
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- Overnight Delivery (2)
Secondary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP31718SU-N | Goat IgG (H+L chain) |
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Mouse | Serum | Gt | C, E, WB |
2 ml /
€205.00
|
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| OriGene Technologies GmbH | |||||||
| AP31796FC-N | Goat IgG (H+L chain) |
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Chicken | Ig | FITC | Gt | C, ICC/IF |
1 ml /
€200.00
|
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| OriGene Technologies GmbH | |||||||
| AP31796HR-N | Goat IgG (H+L chain) |
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Chicken | Ig | HRP | Gt | E, ID, WB |
1 ml /
€240.00
|
|
| OriGene Technologies GmbH | |||||||
| AP31796PU-N | Goat IgG (H+L chain) |
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Chicken | Ig | Aff - Purified | Gt | C, E, ICC/IF, WB |
1 ml /
€180.00
|
|
| OriGene Technologies GmbH | |||||||
| R1254AP | Goat IgG (H+L chain), adsorbed |
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Donkey | AP |
1 mg /
€360.00
|
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| OriGene Technologies GmbH | |||||||
| R1254B | Goat IgG (H+L chain), adsorbed |
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Donkey | Biotin |
1 mg /
€310.00
|
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| OriGene Technologies GmbH | |||||||
| R1254F | Goat IgG (H+L chain), adsorbed |
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Donkey | FITC | Gt |
1 mg /
€295.00
|
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| OriGene Technologies GmbH | |||||||
| R1254HRP | Goat IgG (H+L chain), adsorbed |
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Donkey | HRP |
1 mg /
€315.00
|
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| OriGene Technologies GmbH | |||||||
| R1254P | Goat IgG (H+L chain), adsorbed |
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Donkey | Aff - Purified |
1 mg /
€205.00
|
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| OriGene Technologies GmbH | |||||||
| R1254T | Goat IgG (H+L chain), adsorbed |
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Donkey | TRITC |
1 mg /
€310.00
|
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| OriGene Technologies GmbH | |||||||
| R1254TR | Goat IgG (H+L chain), adsorbed |
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Donkey | Texas Red |
1 mg /
€310.00
|
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| OriGene Technologies GmbH | |||||||
| R1317AP | Goat IgG (H+L chain) |
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Rabbit | AP | C, E, WB |
1 mg /
€250.00
|
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| OriGene Technologies GmbH | |||||||
| R1317B | Goat IgG (H+L chain) |
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Rabbit | Biotin | C, E, WB |
2 mg /
€220.00
|
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| OriGene Technologies GmbH | |||||||
| R1317F | Goat IgG (H+L chain) |
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Rabbit | FITC | E, ICC/IF |
2 mg /
€220.00
|
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| OriGene Technologies GmbH | |||||||
| R1317HRP | Goat IgG (H+L chain) |
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Rabbit | HRP | C, E, WB |
2 mg /
€240.00
|
|||
| OriGene Technologies GmbH | |||||||
| R1317P | Goat IgG (H+L chain) |
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Rabbit | Aff - Purified | C, E, WB |
2 mg /
€205.00
|
|||
| OriGene Technologies GmbH | |||||||
| R1317T | Goat IgG (H+L chain) |
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Rabbit | TRITC | E, ICC/IF |
2 mg /
€220.00
|
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| OriGene Technologies GmbH | |||||||
| R1317TR | Goat IgG (H+L chain) |
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Rabbit | Texas Red |
2 mg /
€230.00
|
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| OriGene Technologies GmbH | |||||||
| R1318AP | Goat IgG (H+L chain), adsorbed |
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Rabbit | AP |
0.5 mg /
€310.00
|
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| OriGene Technologies GmbH | |||||||
| R1318B | Goat IgG (H+L chain), adsorbed |
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Rabbit | Biotin |
1 mg /
€300.00
|
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| OriGene Technologies GmbH | |||||||



