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GOT2 antibody

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Principal name

GOT2 antibody

Alternative names for GOT2 antibody

Aspartate aminotransferase, mitochondrial, mAspAT, Transaminase A, FABP-1, FABPpm, Fatty acid-binding protein

SwissProt ID

P00505 (Human), P00506 (Pig), P00507 (Rat), P05202 (Mouse)

Gene ID

2806 (GOT2), 396968, 14719 (Got2), 25721

Ncbi ID

9606, NP_999093.1, NP_002071, NP_002071.2, AAH00525.1, NP_034455.1, NM_013177

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine), Can (Canine), Ze (Zebrafish), Chk (Chicken), African clawed frog, Eq (Equine), Fi (Fish), Hst (Hamster), Mky (Monkey), Op (Opossum), Pl (Plant), Prim (Primate), Tr (Turkey), Bear, GP (Guinea Pig), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Sheep, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of GOT2 antibody

Aspartate Aminotransferase [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene.
Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.

General readings

Teichberg VI.
From the liver to the brain across the blood-brain barrier.
Proc Natl Acad Sci U S A. 2007 May 1;104(18):7315-6.
PMID: 17463093

H00002806-A01 - GOT2
GOT2 polyclonal antibody (A01), Lot # FHC0061120QCS1. Western Blot analysis of GOT2 expression in NIH/3T3.
H00002806-A01 - GOT2
GOT2 polyclonal antibody (A01), Lot # FHC0061120QCS1 Western Blot analysis of GOT2 expression in A-431 ( Cat # L015V1 ).
H00002806-A01 - GOT2
GOT2 polyclonal antibody (A01), Lot # FHC0061120QCS1. Western Blot analysis of GOT2 expression in Raw 264.7.
H00002806-A01 - GOT2
GOT2 polyclonal antibody (A01), Lot # FHC0061120QCS1. Western Blot analysis of GOT2 expression in PC-12.
AP07532PU-N - GOT2
Skeletal Muscle: Formalin-Fixed Paraffin-Embedded (FFPE)
PAB7440 - GOT2
GOT2 polyclonal antibody ( Cat # PAB7440 ) ( 0.03 µg/ml ) staining of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
PAB7559 - GOT2
GOT2 polyclonal antibody ( Cat # PAB7559 ) ( 0.3 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
ARP43517_T100 - GOT2
Human 293T; WB Suggested Anti-GOT2 Antibody Titration: 1.0ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; GOT2 antibody - N-terminal region (ARP43517_T100) in Human 293T cells using Western Blot
ARP43518_T100 - GOT2
Human Liver; GOT2 antibody - C-terminal region (ARP43518_T100) in Human Liver cells using Immunohistochemistry
ARP43518_T100 - GOT2
Human HepG2; WB Suggested Anti-GOT2 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. GOT2 is supported by BioGPS gene expression data to be expressed in HepG2.; GOT2 antibody - C-terminal region (ARP43518_T100) in Human HepG2 cells using Western Blot
H00002806-M09 - GOT2
GOT2 monoclonal antibody (M09), clone 4H8. Western Blot analysis of GOT2 expression in HepG2(Cat # L019V1 ).
AP08818PU-N - GOT2
AP08818PU-N staining GOT2 in paraffin-embedded Human Liver by Immunohistochemistry.
AP08818PU-N - GOT2
Antibody (AP08818PU-N at 0.03 µg/ml) staining of Human Kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by Chemiluminescence.
ARP43517_P050 - GOT2
Human 293T; WB Suggested Anti-GOT2 Antibody. . Titration: 1 ug/ml. . Positive Control: 293T Whole Cell; GOT2 antibody - N-terminal region (ARP43517_P050) in Human 293T cells using Western Blot
AM06383SU-N - GOT2
Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
AM06383SU-N - GOT2
Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
AR50767PU-N - GOT2
TA303304 - GOT2
TA303304 (0.3µg/ml) staining of Human Liver cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA303325 - GOT2
TA303325 (0.03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA325088 - GOT2
Western blot analysis of lysates from Hela, HepG2, HL-60, Ramos, mouse brain cell line (from left to right), using GOT2 Antibody (N-term)(Cat. #TA325088). TA325088 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
TA325088 - GOT2
GOT2 Antibody (N-term) (Cat. #TA325088) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA327757 - GOT2
Anti-GOT2 antibody (0.3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA332130 - GOT2
WB Suggested Anti-GOT2 Antibody; Titration: 1 ug/ml; Positive Control: 293T Whole Cell
TA332131 - GOT2
WB Suggested Anti-GOT2 Antibody Titration: 1.0ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysate
TA350033 - GOT2
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-7: 293T cells, Hela cells, Human fetal liver tissue, mouse spleen tissue, Mouse heart tissue, human fetal brain tissue, hepatocellular carcinoma tissue, Primary antibody: (GOT2 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
TA350033 - GOT2
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (GOT2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
TA350033 - GOT2
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (GOT2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GOT2 antibody
  Goat Polyclonal Antibody against GOT2  
TA303304 (0.3µg/ml) staining of Human Liver cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

GOT2 antibody
  Goat Polyclonal Antibody against GOT2 (Internal Region)  
TA303325 (0.03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

GOT2 antibody
  Rabbit polyclonal GOT2 Antibody (N-term)  
Western blot analysis of lysates from Hela, HepG2, HL-60, Ramos, mouse brain cell line (from left to right), using GOT2 Antibody (N-term)(Cat. #TA325088). TA325088 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Rabbit IgG Purified Bov, Chk, Hu, Mky, Ms, Por, Rt F, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

GOT2 antibody
  GOT2 Goat Polyclonal Antibody  
Anti-GOT2 antibody (0.3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

GOT2 antibody
  Rabbit Polyclonal Anti-GOT2 Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-7: 293T cells, Hela cells, Human fetal liver tissue, mouse spleen tissue, Mouse heart tissue, human fetal brain tissue, hepatocellular carcinoma tissue, Primary antibody: (GOT2 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

GOT2 antibody
Skeletal Muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Sheep Purified Hu, Por ICC/IF, IP, P, WB
50 µl / €425.00
  OriGene Technologies GmbH

GOT2 (295-306) antibody
AP08818PU-N staining GOT2 in paraffin-embedded Human Liver by Immunohistochemistry. Goat IgG Aff - Purified Eq, Hst, Hu, Mky E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+1 additional image

7 Item(s)

per page

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Product Type
  1. Primary Antibody (7)
Target
  1. GOT2 (7)
Property
  1. 295-306 (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (5)
  3. Rat (Rt) (5)
  4. Porcine (Por) (3)
  5. Bovine (Bov) (2)
  6. Monkey (Mky) (2)
  7. Canine (Can) (1)
  8. Chicken (Chk) (1)
  9. Equine (Eq) (1)
  10. Hamster (Hst) (1)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Enzyme Immunoassay (E) (4)
  3. Paraffin Sections (P) (3)
  4. Flow cytometry (F) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  6. Immunoprecipitation (IP) (1)
Presentation
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  2. Aff - Purified (3)
Host
  1. Goat (4)
  2. Rabbit (2)
  3. Sheep (1)
Images
  1. 1 image (7)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Paraffin Sections (P) (2)
  3. IHC (1)
Manufacturer
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