GPR116 antibody
Principal name
GPR116 antibody
Alternative names for GPR116 antibody
G-protein coupled receptor 116, KIAA0758
Gene ID
221395 (GPR116)
Ncbi ID
NP_001091988.1, NP_001091988, ACE87667.1, NM_015234, NM_001098518
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB)
Background of GPR116 antibody
- Kit Component:
- - KN209170G1, ADGRF5 gRNA vector 1 in pCas-Guide vector
- KN209170G2, ADGRF5 gRNA vector 2 in pCas-Guide vector
- KN209170D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Human Heart (formalin-fixed, paraffin-embedded) stained with GPR116 antibody AP22833PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Colon (formalin-fixed, paraffin-embedded) stained with GPR116 antibody AP22833PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of GPR116 expression in HeLa (A); Y79 (B) whole cell lysates.
Immunohistochemical analysis of GPR116 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of GPR116 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using GPR116 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using GPR116 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from A549 cells, using GPR116 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using GPR116 antibody.The picture on the right is treated with the synthesized peptide.
Anti-GPR116 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human brain, neuron. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Adrenal, Pheochromocytoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human brain, neuron. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR116 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA316255 | GPR116 antibody |
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| Rabbit polyclonal anti-GPR116 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA316256 | GPR116 antibody |
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| Rabbit polyclonal anti-GPR116 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA340429 | GPR116 (N-term) antibody |
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| Rabbit Polyclonal Anti-GPR116 Antibody (N-Terminus) | |||||||
 |
Rabbit | Aff - Purified | Hu, Mky | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA340430 | GPR116 (3rd Cytopl. Dom.) antibody |
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| Rabbit Polyclonal Anti-GPR116 Antibody (Cytoplasmic Domain) | |||||||
|
Rabbit | Aff - Purified | Ant, Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Rb, Rt | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA340820 | GPR116 antibody |
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| Rabbit Polyclonal Anti-GPR116 Antibody (Internal) | |||||||
 |
Rabbit | Purified | Hu, Mky |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP22833PU-N | GPR116 (Internal) antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | E, P |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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