GPRK5 antibody
Principal name
GPRK5 antibody
Alternative names for GPRK5 antibody
G protein-coupled receptor kinase 5, GRK5
Ncbi ID
9606, NP_005299.1, NP_005299, NM_005308, NP_061357.3, NM_030829
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of GPRK5 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
Western blot (WB) analysis of GRK5 antibody (Cat.-No.: AP06682PU-N) in extracts from HeLa cells.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GRK5 polyclonal antibody ( Cat # PAB4519 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
Placenta: Formalin-Fixed Paraffin Embedded (FFPE)
Human GRK5, Rat striatum; . Lanes: Lane1: 1ug human GRK5 protein. Lane2: 0.25ug human GRK5 protein. Lane3: 0.1ug human GRK5 protein. Lane4: 5ug rat striatum homogenate. Lane5: 5ug rat striatum cytosol. Lane6: 5ug rat striatum light membrane fraction. Lane7: 5ug rat striatum synaptic membrane fraction. Lane8: 5ug rat striatum crude synaptic vesicle fraction. Lane9: 5ug rat striatum homogenate. Lane10: 5ug rat striatum cytosol. Lane11: 5ug rat striatum light membrane fraction. Lane12: 5ug rat striatum synaptic membrane fraction. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:15000. Gene Name: GRK5. Submitted by: Eugenia V Gurevich, Vanderbilt University.; GRK5 antibody - middle region (ARP54750_P050) in Human GRK5, Rat striatum cells using Western Blot
tobacco hornworm; . Lanes: Lanes 1-3: 40 ug tobacco hornworm larvae intestine lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-rabbit HRP. Secondary Antibody Dilution: 1:10000. Gene Name: GRK5. Submitted by: Dra. Helena Porta Ducoing and Biol. Gladys Jimenez Nopala.; GRK5 antibody - middle region (ARP54750_P050) in tobacco hornworm cells using Western Blot
mouse left ventricle heart; . Sample Type :. Lane 1: 20ug mouse left ventricle heart lysate . Primary Antibody Dilution :. 1:1000 . Secondary Antibody:. Anti-rabbit-HRP . Secondary Antibody Dilution:. 1:5000 . Color/Signal Descriptions:. GRK5 . Gene Name:. Kathleen Gabrielson . Submitted by:.; GRK5 antibody - middle region (ARP54750_P050) in mouse left ventricle heart cells using Western Blot
Human Brain; WB Suggested Anti-GRK5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; GRK5 antibody - middle region (ARP54750_P050) in Human Brain cells using Western Blot
Immunohistochemical analysis of GRK5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of GRK5 expression in H9C2 (A); HEK293T (B); HeLa (C) whole cell lysates.
Adult rat brain membrane immunoblot
Immunoblot against adult rat brain membrane (RBM) and extracts of COS cells transiently transfected with untagged GRK5, GRK6 or Kv2.1 plasmid and probed with N145/20 TC supe.
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N145/20 (left) or N52A/42 (right) TC supe.
Adult rat brain membrane immunoblot
Immunoblot against adult rat brain membrane (RBM) and extracts of COS cells transiently transfected with untagged GRK5, GRK6 or Kv2.1 plasmid and probed with N145/20 TC supe.
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N145/20 (left) or N52A/42 (right) TC supe.
Western blot analysis of extracts from HUVEC cells, HepG2 cells and HeLa cells, using GRK5 antibody.The lane on the right is treated with the synthesized peptide.
Anti-GRK5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GRK5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GRK5 antibody IHC of human myocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GRK5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western blot analysis of anti-GRK5 Pab (Cat. #TA324891) in mouse brain tissue lysate. GRK5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK5 Antibody (C-term) (Cat.#TA324891), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
WB Suggested Anti-GRK5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human brain
Lanes: Lane1: 1ug human GRK5 protein; Lane2: 0.25ug human GRK5 protein; Lane3: 0.1ug human GRK5 protein; Lane4: 5ug rat striatum homogenate; Lane5: 5ug rat striatum cytosol; Lane6: 5ug rat striatum light membrane fraction; Lane7: 5ug rat striatum synaptic
Lanes: Lanes 1-3: 40 ug tobacco hornworm larvae intestine lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Goat anti-rabbit HRP; Secondary Antibody Dilution: 1: 10000; Gene Name: GRK5; Submitted by: Dra. Helena Porta Ducoing and Biol. Glady
Sample Type: Lane 1: 20ug mouse left ventricle heart lysate Primary Antibody Dilution: 1: 1000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1: 5000 Color/Signal Descriptions: GRK5 Gene Name: Kathleen Gabrielson Submitted by:
Host: Rabbit; Target Name: GRK5; Sample Tissue: 721_B Whole cell lysates; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA312217 | GPRK5 antibody |
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| Rabbit polyclonal anti-GRK5 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA317672 | GPRK5 antibody |
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| GRK5 Rabbit Polyclonal (Internal) Antibody | |||||||
 |
Rabbit | IgG | Purified | Bt, Can, Eq, Gorilla, Hu, Mky, Ms, Rt |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA317673 | GPRK5 antibody |
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| GRK5 Rabbit Polyclonal (N-Terminus) Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Gorilla, Hu, Ms, Por, Rt |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA317678 | GPRK5 (N-term) antibody |
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| GRK5 Rabbit Polyclonal (N-Terminus) Antibody | |||||||
 |
Rabbit | Aff - Purified | Bear, Bov, Can, Elephant, Eq, Hu, Por, Prim | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA317830 | GPRK5 (Internal) antibody |
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| GRK5 Rabbit Polyclonal (Internal) Antibody | |||||||
|
Rabbit | IgG | Aff - Purified | Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rt, Tr, Xen | P |
50 µg /
€425.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324891 | GPRK5 (C-term) antibody |
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| Rabbit polyclonal GRK5 Antibody (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP06682PU-N | GPRK5 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| AP08295PU-N | GPRK5 (350-400) antibody |
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 |
Rabbit | IgG | Aff - Purified | Bov, Chk, Hu, Ms, Pufferfish, Rt, Ze | P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| 73-179 | Pan GRK (C-term) antibody |
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Mouse | IgG1 | N145/20 | Supernatant | Hu, Ms, Rt | ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
| +2 additional images |
|
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| 75-179 | Pan-GRK (C-term) antibody |
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 |
Mouse | IgG1 | N145/20 | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +2 additional images |
|
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN209137 | GRK5 - human gene knockout kit via CRISPR |
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|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209137BN | GRK5 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309137 | GPRK5 |
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| Recombinant protein of human G protein-coupled receptor kinase 5 (GRK5) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401636 | GRK5 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR202951L2V | Lenti ORF particles, Grk5 (GFP-tagged ORF) - Rat G protein-coupled receptor kinase 5 (Grk5), 200 uL, >10^7 TU/mL |
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200 µl /
€1,320.00
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| OriGene Technologies, Inc. | ||
| RR202951L1V | Lenti ORF particles, Grk5 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor kinase 5 (Grk5), 200 uL, >10^7 TU/mL |
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200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||
