GRK4 antibody
Principal name
GRK4 antibody
Alternative names for GRK4 antibody
G protein-coupled receptor kinase 4, ITI1, GPRK2L, GPRK4, G protein-coupled receptor kinase GRK4
Ncbi ID
9606, NP_001004057, NP_001004056.1, ADR83217.1, NP_892027.2, NM_182982, NP_892027, NM_022928, NM_001004057, NM_001004056
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of GRK4 antibody
- Kit Component:
- - KN215579G1, GRK4 gRNA vector 1 in pCas-Guide vector
- KN215579G2, GRK4 gRNA vector 2 in pCas-Guide vector
- KN215579D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Staessen,J.A., (er) Hypertension (2008) In press
Detection limit for recombinant GST tagged GRK4 is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GRK4 is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GRK4 is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GRK4 is approximately 0.3ng/ml as a capture antibody.
GRK4 polyclonal antibody (A01), Lot # 060516JCS1 Western Blot analysis of GRK4 expression in HL-60 ( Cat # L014V1 ).
Human Jurkat; WB Suggested Anti-GRK4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; GRK4 antibody - middle region (ARP53565_P050) in Human Jurkat cells using Western Blot
Human MCF-7; WB Suggested Anti-GRK4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate; GRK4 antibody - middle region (ARP53566_P050) in Human MCF-7 cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRK4 (RC216602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRK4.(1:500)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRK4 (RC216602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRK4.
Predicted band size: 66 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm . Positive control: Human tonsil tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using GRK4 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted band size: 66 kDa . Positive control: Hela and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds)
Predicted cell location: Cytoplasm . Positive control: Human liver cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using GRK4 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-GRK4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
WB Suggested Anti-GRK4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: MCF7 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323501 | GRK4 antibody |
||||||
| Anti-GRK4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA323502 | GRK4 antibody |
||||||
| Anti-GRK4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA805974 | GRK4 antibody |
||||||
| GRK4 mouse monoclonal antibody, clone OTI4F5 (formerly 4F5) | |||||||
 |
Mouse | IgG1 | OTI4F5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA806069 | GRK4 antibody |
||||||
| GRK4 mouse monoclonal antibody, clone OTI9A3 (formerly 9A3) | |||||||
 |
Mouse | IgG2a | OTI9A3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP316602 | GRK4 (transcript variant 1) |
||||
| Recombinant protein of human G protein-coupled receptor kinase 4 (GRK4), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY405296 | GRK4 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204470L2V | Lenti ORF particles, Grk4 (GFP-tagged ORF) - Rat G protein-coupled receptor kinase 4 (Grk4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,290.00
|
|
| OriGene Technologies, Inc. | ||
| RR204470L1V | Lenti ORF particles, Grk4 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor kinase 4 (Grk4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,160.00
|
|
| OriGene Technologies, Inc. | ||
