GSTO1 antibody
Principal name
GSTO1 antibody
Alternative names for GSTO1 antibody
GSTO-1, GSTTLP28, Glutathione S-transferase omega-1
Ncbi ID
9606, NP_004823, NP_004823.1, NM_001191002, NP_034492.1, NM_010362, NM_004832, NM_001191003
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Paraffin Sections (P), Flow Cytometry (F)
Background of GSTO1 antibody
- Kit Component:
- - KN307401G1, Gsto1 gRNA vector 1 in pCas-Guide vector
- KN307401G2, Gsto1 gRNA vector 2 in pCas-Guide vector
- KN307401D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ruiz-Romero C., et al. (2008) Proteomics. 8(3):495-507.
Detection limit for recombinant GST tagged GSTO1 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of GSTO1 expression in transfected 293T cell line by GSTO1 polyclonal antibody (A01).Lane1:GSTO1 transfected lysate(27.566 KDa).Lane2:Non-transfected lysate.
GSTO1 polyclonal antibody (A01), Lot # 051214JC01 Western Blot analysis of GSTO1 expression in HepG2 ( Cat # L019V1 ).
Detection limit for recombinant GST tagged GSTO1 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to GSTO1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of GSTO1 expression in transfected 293T cell line (H00009446-T01) by GSTO1 MaxPab polyclonal antibody.Lane 1: GSTO1 transfected lysate(26.51 KDa).Lane 2: Non-transfected lysate.
GSTO1 MaxPab polyclonal antibody. Western Blot analysis of GSTO1 expression in NIH/3T3.
GSTO1 MaxPab polyclonal antibody. Western Blot analysis of GSTO1 expression in Jurkat.
GSTO1 MaxPab polyclonal antibody. Western Blot analysis of GSTO1 expression in PC-12.
GSTO1 MaxPab polyclonal antibody. Western Blot analysis of GSTO1 expression in HeLa.
GSTO1 MaxPab rabbit polyclonal antibody. Western Blot analysis of GSTO1 expression in human liver.
Western Blot analysis of GSTO1 expression in transfected 293T cell line (H00009446-T02) by GSTO1 MaxPab polyclonal antibody.Lane 1: GSTO1 transfected lysate(27.60 KDa).Lane 2: Non-transfected lysate.
GSTO1 MaxPab rabbit polyclonal antibody. Western Blot analysis of GSTO1 expression in Jurkat.
Immunofluorescence of purified MaxPab antibody to GSTO1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of GSTO1 expression in transfected 293T cell line (H00009446-T01) by GSTO1 MaxPab polyclonal antibody.Lane 1: GSTO1 transfected lysate(26.51 KDa).Lane 2: Non-transfected lysate.
GSTO1 MaxPab polyclonal antibody. Western Blot analysis of GSTO1 expression in NIH/3T3.
GSTO1 MaxPab polyclonal antibody. Western Blot analysis of GSTO1 expression in Jurkat.
GSTO1 MaxPab polyclonal antibody. Western Blot analysis of GSTO1 expression in PC-12.
GSTO1 MaxPab polyclonal antibody. Western Blot analysis of GSTO1 expression in HeLa.
Recombinant human GSTO1, 1-241 aa: 15% SDS-PAGE (3ug)
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. TA308286 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded GSTA2 xenograft, using GSTO1(TA308286) antibody at 1:100 dilution.
Western blot analysis of GSTO1 Antibody (Center) (Cat. #TA324757) in NCI-H460 cell line lysates (35ug/lane). GSTO1 (arrow) was detected using the purified Pab.
Western blot analysis of GSTO1 (arrow) using rabbit polyclonal GSTO1 Antibody (Center) (Cat. #TA324757). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTO1 gene.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with GSTO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
GSTO1 Antibody (Center) (Cat. #TA324757) flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA329073 (0.5µg/ml) staining of Human Liver (A) and Peripheral Blood Lymphocytes (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Gel: 8%SDS-PAGE, Lysate: 50 ug, Lane: Human liver cancer tissue, Primary antibody: (GSTO1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (GSTO1 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (GSTO1 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308286 | GSTO1 antibody |
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| Rabbit Polyclonal antibody to GSTO1 (glutathione S-transferase omega 1) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA324757 | GSTO1 antibody |
||||||
| Rabbit polyclonal GSTO1 Antibody (Center) | |||||||
 |
Rabbit | IgG | Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
|||||||
| TA329073 | GSTO1 antibody |
||||||
| GSTO1 Goat Polyclonal Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350038 | GSTO1 antibody |
||||||
| Rabbit Polyclonal Anti-GSTO1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300362 | GSTO1 |
||||
| Recombinant protein of human glutathione S-transferase omega 1 (GSTO1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY434052 | GSTO1 overexpression lysate |
|
 |
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202949L2V | Lenti ORF particles, Gsto1 (GFP-tagged) - Mouse glutathione S-transferase omega 1 (Gsto1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR202949L1V | Lenti ORF particles, Gsto1 (Myc-DDK-tagged) - Mouse glutathione S-transferase omega 1 (Gsto1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||