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GTF2H1 / BTF2 antibody

Principal name

GTF2H1 / BTF2 antibody

Alternative names for GTF2H1 / BTF2 antibody

BTF2-p62, TFIIH, General transcription factor IIH subunit 1, General transcription factor IIH polypeptide 1, TFIIH basal transcription factor complex p62 subunit, Basic transcription factor 2 62 kDa subunit

SwissProt ID

P32780 (Human), Q9DBA9 (Mouse)

Gene ID

2965 (GTF2H1), 14884 (Gtf2h1)

Ncbi ID

9606, NP_005307, NP_001135779, NP_001135779.1, NM_005316, NM_001142307, NP_032212.3, NM_008186

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of GTF2H1 / BTF2 antibody

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription actors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Immunoperoxidase of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.[antibody concentration 5 ug/ml]

H00002965-M01

Immunofluorescence of monoclonal antibody to GTF2H1 on HeLa cell. [antibody concentration 10 ug/ml]

H00002965-M01

Western blot analysis of GTF2H1 over-expressed 293 cell line, cotransfected with GTF2H1 Validated Chimera RNAi ( Cat # H00002965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (Cat # H00002965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

H00002965-M01

GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Western Blot analysis of GTF2H1 expression in Hela NE ( Cat # L013V3 ).

H00002965-M01

GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Western Blot analysis of GTF2H1 expression in Jurkat ( Cat # L017V1 ).

H00002965-M01

Western Blot analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.

H00002965-M01

Detection limit for recombinant GST tagged GTF2H1 is approximately 1ng/ml as a capture antibody.

H00002965-M01

Detection limit for recombinant GST tagged GTF2H1 is approximately 0.1ng/ml as a capture antibody.

H00002965-M02

Human kidney; GTF2H1 antibody - N-terminal region (P100811_T100) in Human kidney cells using Immunohistochemistry

P100811_T100

Human Liver; GTF2H1 antibody - N-terminal region (P100811_T100) in Human Liver cells using Immunohistochemistry

P100811_T100

Human Daudi; WB Suggested Anti-GTF2H1 Antibody Titration: 1.0-2.0ug/ml. Positive Control: Daudi cell lysate. GTF2H1 is supported by BioGPS gene expression data to be expressed in Daudi.; GTF2H1 antibody - N-terminal region (P100811_T100) in Human Daudi cells using Western Blot

P100811_T100

Immunoprecipitation of GTF2H1 transfected lysate using anti-GTF2H1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (H00002965-M01).

H00002965-D01

Human ACHN; WB Suggested Anti-GTF2H1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate. GTF2H1 is supported by BioGPS gene expression data to be expressed in ACHN.; GTF2H1 antibody - middle region (P100929_P050) in Human ACHN cells using Western Blot

P100929_P050

Immunohistochemistry analyzes of TFIIH p62 antibody (Cat.-No.: AP20413PU-N) in paraffin-embedded human brain tissue.

AP20413PU-N

Western blot analysis of TFIIH p62 antibody (Cat.-No.: AP20413PU-N) in extracts from Jurkat cells.

AP20413PU-N

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TF2H1 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, using TF2H1 Antibody. The lane on the right is treated with the synthesized peptide.

TP301341

Western blot analysis of extracts from Jurkat cells, using TF2H1 antibody.The lane on the right is treated with the synthesized peptide.

TA313983

Immunohistochemistry analysis of paraffin-embedded human brain tissue using TF2H1 antibody.The picture on the right is treated with the synthesized peptide.

TA313983

WB Suggested Anti-GTF2H1 Antibody Titration: 1.0-2.0ug/ml; Positive Control: Daudi cell lysateGTF2H1 is supported by BioGPS gene expression data to be expressed in Daudi

TA330081

Human kidney

TA330081

Human Liver

TA330081

WB Suggested Anti-GTF2H1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: ACHN cell lysateGTF2H1 is supported by BioGPS gene expression data to be expressed in ACHN

TA330140

Western blot detection of GTF2H1 in Hela,Hela NE and Jurkat cell lysates using GTF2H1 mouse mAb (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.

TA346957

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using GTF2H1 mouse mAb (dilution 1:50).

TA346957

Immunoprecipitation analysis of Hela cell lysates using GTF2H1 mouse mAb.

TA346957

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA313983	GTF2H1 / BTF2 antibody
	Rabbit polyclonal anti-TF2H1 antibody
	Rabbit	IgG		Purified	Hu, Ms	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA346957	GTF2H1 / BTF2 antibody
	Mouse Monoclonal GTF2H1 Antibody
	Mouse	IgG1	3E8-D3-H8	Purified		ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
AP20413PU-N	GTF2H1 / BTF2 antibody
	Rabbit			Aff - Purified	Hu, Ms	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP301341	GTF2H1 / BTF2 (transcript variant 1)
	Recombinant protein of human general transcription factor IIH, polypeptide 1, 62kDa (GTF2H1), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY417388	GTF2H1 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY428028	GTF2H1 overexpression lysate
		0.1 mg / €315.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR208340L2V	Lenti ORF particles, Gtf2h1 (GFP-tagged) - Mouse general transcription factor II H, polypeptide 1 (Gtf2h1), 200 uL, >10^7 TU/mL
		200 µl / €1,250.00
		OriGene Technologies, Inc.

MR208340L1V	Lenti ORF particles, Gtf2h1 (Myc-DDK-tagged) - Mouse general transcription factor II H, polypeptide 1 (Gtf2h1), 200 uL, >10^7 TU/mL
		200 µl / €1,120.00
		OriGene Technologies, Inc.

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