HDAC3 antibody
Principal name
HDAC3 antibody
Alternative names for HDAC3 antibody
Histone deacetylase 3, RPD3-2, SMAP45, HD3
Ncbi ID
NP_003874.2, 9606, NP_003874, NP_445900.1, NM_003883, NM_053448
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), ChIP assay (ChIP), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of HDAC3 antibody
Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to D. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).HDAC3 is a nuclear and cytoplasmic protein that deacetylates both histone (H2A, H3, H4) and non-histone substrates (RelA, SRY, p53, MEF2, PCAF and p300/CBP) (8). HDAC3 deacetylase activity is stimulated by interactions with the N-CoR and SMRT co-repressor proteins. Together, these three proteins form a functiol complex that represses transcription associated with nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD and Pit-1 (8,9). Phosphorylation of HDAC3 on Ser424 by casein kise 2 (CK2) also increases HDAC3 deacetylase activity (9). Subsequently, de-phosphorylation by protein phosphatase 4 (PP4) decreases HDAC3 activity (9).1. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.2. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.3. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.4
Western blot analysis of HDAC-3 expression in Jurkat cell lysate.
HDAC3 monoclonal antibody (M02), clone 3E11. Western Blot analysis of HDAC3 expression in NIH/3T3 ( Cat # L018V1 ).
HDAC3 monoclonal antibody (M02), clone 3E11. Western Blot analysis of HDAC3 expression in Raw 264.7 ( Cat # L024V1 ).
HDAC3 monoclonal antibody (M02), clone 3E11 Western Blot analysis of HDAC3 expression in Hela NE ( Cat # L013V3 ).
HDAC3 monoclonal antibody (M02), clone 3E11. Western Blot analysis of HDAC3 expression in PC-12 ( Cat # L012V1 ).
Western Blot analysis of HDAC3 expression in transfected 293T cell line (H00008841-T01) by HDAC3 MaxPab polyclonal antibody.Lane 1: HDAC3 transfected lysate(47.08 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of HDAC3 in HeLa cell lysate with HDAC3 polyclonal antibody ( Cat # PAB0171 ) .
Immunofluorescence of monoclonal antibody to HDAC3 on HeLa cell. [antibody concentration 10 ug/ml]
HDAC3 monoclonal antibody (M03), clone 2A3. Western Blot analysis of HDAC3 expression in NIH/3T3(Cat # L018V1 ).
HDAC3 monoclonal antibody (M03), clone 2A3. Western Blot analysis of HDAC3 expression in Raw 264.7(Cat # L024V1 ).
HDAC3 monoclonal antibody (M03), clone 2A3 Western Blot analysis of HDAC3 expression in Hela NE ( Cat # L013V3 ).
HDAC3 monoclonal antibody (M03), clone 2A3. Western Blot analysis of HDAC3 expression in PC-12(Cat # L012V1 ).
Placenta: Formalin-Fixed Paraffin-Embedded (FFPE)
Western Blot analysis of HDAC3 expression in transfected 293T cell line (H00008841-T02) by HDAC3 MaxPab polyclonal antibody.Lane 1: HDAC3 transfected lysate(48.80 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of HDAC3 expression in Jurkat cell lysate. The antibody detects the 49 kDa HDAC3.
Immunoprecipitation of HDAC3 transfected lysate using anti-HDAC3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HDAC3 MaxPab mouse polyclonal antibody (B01) (H00008841-B01).
Immunohistochemistry analyzes of SMAP45 antibody (AP20235PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of SMAP45 antibody (Cat.-No.: AP20235PU-N) in extracts from PC12 cells.
Brain, cerebellum, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Skin, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis using anti-HDAC3 monoclonal antibody against truncated Trx-HDAC3 recombinant protein(Lane 1), full length HDAC3-hIgGFc (aa1-428) transfected CHO-K1 cell lysate (Lane 2) and Hela cell lysate (Lane 3).
Rat Liver; WB Suggested Anti-Hdac3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Rat Liver; Hdac3 antibody - C-terminal region (ARP42786_P050) in Rat Liver cells using Western Blot
IHC Image of AM20548SU-NBrain, cerebellum, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Image of AM20548SU-NBrain, cortex, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis using anti-HDAC3 mAb (AM20548SU-N) against Hela (1), NIH/3T3 (2), C6 (3) and COS (4) cell lysate.
Confocal Immunofluorescence analysis of Hela cells using HDAC3 antibody Cat.-No AM06267SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Western blot analysis using HDAC3 antibody Cat.-No AM06267SU-N against Hela (Lane 1), NIH/3T3 (Lane 2), C6 (Lane 3) and COS (Lane 4) cell lysate.
Immunohistochemical analysis of paraffin-embedded human esophagus cancer (left) and breast carcinoma tissue (right), showing nuclear localization with DAB staining using HDAC3 antibody Cat.-No AM06268SU-N
Western blot analysis using HDAC3 antibody Cat.-No AM06268SU-N against truncated Trx-HDAC3 recombinant protein (Lane 1), full length HDAC3-hIgGFc (aa1-428) transfected CHO-K1 cell lysate(Lane 2) and Hela cell lysate (Lane 3).
Immunohistochemical analysis of Histone Deacetylase 3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Histone Deacetylase 3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Histone Deacetylase 3 expression in HeLa (A); C32 (B); NIH3T3 (C); Raw264.7 (D); PC12 (E); rat heart (F) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC3 (RC200605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC3.
Western blot analysis of Histone Deacetylase 3 (pS424) expression in Hela (A); K562 (B); NIH3T3 (C) whole cell lysates.
Immunohistochemical analysis of Histone Deacetylase 3 (pS424) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Histone Deacetylase 3 (pS424) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
AP33509PU-N (0.1µg/ml) staining of Jurkat nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 49 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Western blot analysis of A549, NIH/3T3, Raji, 293T and Hela cell using TA321609 (HDAC3 Antibody) at dilution 1/200
Western blot analysis of HDAC3 expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts from HepG2 cells, using HDAC3 antibody .The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using HDAC3 Antibody. The lane on the left is treated with systhesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC3 antibodyThe picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of COS7 cells, using HDAC3 antibody .The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts of various cell lines, using HDAC3 antibody.
Immunohistochemistry of paraffin-embedded rat brain using HDAC3 antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HDAC3 antibody at dilution of 1:200 (400x lens).
Western Blot: HDAC3 Antibody [TA336265] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.
Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [TA336265] - HDAC3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was used. Image objective 40x.
Western blot detection of HDAC3 in THP-1,LNCAP,MCF7,PC12,293,Jurkat,3T3 and Hela cell lysates using HDAC3 mouse mAb (1:1000 diluted).Predicted band size:49KDa.Observed band size:49KDa.
Western blot analysis on NIH-3T3 cell lysate using HDAC3 Antibody
WB Suggested Anti-Hdac3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Rat Liver
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA326123 | HDAC3 antibody |
||||||
| Rabbit polyclonal HDAC3 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
||||||
| TA321608 | HDAC3 antibody |
||||||
| Anti-HDAC3 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321609 | HDAC3 antibody |
||||||
| Anti-HDAC3 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325513 | HDAC3 antibody |
||||||
| Rabbit Polyclonal HDAC3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325514 | HDAC3 antibody |
||||||
| Rabbit Polyclonal HDAC3 (Ser424) Antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327293 | HDAC3 antibody |
||||||
| Rabbit anti-HDAC3 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA336265 | HDAC3 antibody |
||||||
| Rabbit Polyclonal HDAC3 Antibody | |||||||
![Western Blot: HDAC3 Antibody [TA336265] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-1669-1-W.jpg) |
Rabbit | Purified | Hu | C, ChIP, ICC/IF, IP, P, WB |
0.1 mg /
€415.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA346962 | HDAC3 antibody |
||||||
| Mouse Monoclonal HDAC3 Antibody | |||||||
 |
Mouse | IgG1 | 3G3-H6-H10 | Purified | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347765 | HDAC3 antibody |
||||||
| Rabbit Polyclonal Anti-HDAC3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA806191 | HDAC3 antibody |
||||||
| HDAC3 mouse monoclonal antibody, clone OTI4E10 (formerly 4E10) | |||||||
 |
Mouse | IgG2a | OTI4E10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| AM06267SU-N | HDAC3 antibody |
||||||
 |
Mouse | IgG2a | 7G6C5 | Ascites | Hu | ICC/IF, WB |
0.1 ml /
€420.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AM06268SU-N | HDAC3 antibody |
||||||
 |
Mouse | IgG1 | 3A7B5 | Ascites | Hu | E, P, WB |
0.1 ml /
€420.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AM20439SU-N | HDAC3 (224-428) antibody |
||||||
 |
Mouse | IgG1 | 3A7B5 | Ascites | Hu | E, P, WB |
50 µl /
€425.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AM20548SU-N | HDAC3 antibody |
||||||
 |
Mouse | IgG2a | 7G6C5 | Ascites | Hu | E, ICC/IF, P, WB |
50 µl /
€425.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP08439PU-N | HDAC3 (2-17) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu | IP, P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| AP20235PU-N | HDAC3 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP33509PU-N | HDAC3 antibody |
||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | WB |
0.1 mg /
€380.00
|
||
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN200605 | HDAC3 - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200605BN | HDAC3 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200605LP | HDAC3 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||