Hemoglobin beta chain antibody
Principal name
Hemoglobin beta chain antibody
Alternative names for Hemoglobin beta chain antibody
Beta-globin, HBB
Gene ID
3043 (HBB)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), ELISA (detection) (E(detection))
Background of Hemoglobin beta chain antibody
- Kit Component:
- - KN203258G1, HBB gRNA vector 1 in pCas-Guide vector
- KN203258G2, HBB gRNA vector 2 in pCas-Guide vector
- KN203258D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged HBB is approximately 0.3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to HBB on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged HBB is approximately 0.1ng/ml as a capture antibody.
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged HBB is approximately 0.1 ng/ml as a capture antibody.
Western blot analysis in K562 cell line lysates (35ug/lane) using HBB Antibody (C-term) Cat.-No AP52007PU-N. This demonstrates the HBB antibody detected the HBB protein (arrow).
HBB Antibody (C-term) (Cat.-No AP52007PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HBB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes : Anti-HBB Antibody (C-term) at 1:2000 dilution Lane 1: human heart lysate Lane 2: human liver lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometric analysis of K562 cells using HBB Antibody (C-term) Cat.-No AP52007PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM20893PU-N | Hemoglobin beta chain (38-148) antibody |
||||||
 |
Mouse | IgG1 | 7B12 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303258 | Hemoglobin beta chain |
||||
| Recombinant protein of human hemoglobin, beta (HBB) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||