HHLA2 antibody
Principal name
HHLA2 antibody
Alternative names for HHLA2 antibody
HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2
SwissProt ID
Q9UM44 (Human)
Gene ID
11148 (HHLA2)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P)
Background of HHLA2 antibody
- Kit Component:
- - KN205909G1, HHLA2 gRNA vector 1 in pCas-Guide vector
- KN205909G2, HHLA2 gRNA vector 2 in pCas-Guide vector
- KN205909D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of HHLA2 expression in transfected 293T cell line (H00011148-T02) by HHLA2 MaxPab polyclonal antibody.Lane 1: HHLA2 transfected lysate(45.54 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma reacted with AP52042PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
All lanes: Anti-HHLA2 Antibody (N-term) Cat.-No AP52042PU-N at 1/2000 dilution.
Lane 1: Human pancreas lysate. Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: Human pancreas lysate. Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of HHLA2 Antibody (N-term) Cat.-No AP52042PU-N in MDA-MB453, CEM, HL-60, MDA-MB231 cell line lysates (35ug/lane). This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).
Flow cytometric analysis of MDA-MB231 cells using AP52042PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52042PU-N | HHLA2 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +5 additional images |
Highly validated antibody! |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205909 | HHLA2 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205909BN | HHLA2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205909LP | HHLA2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205909RB | HHLA2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||