HIBCH antibody
Principal name
HIBCH antibody
Alternative names for HIBCH antibody
HIB-CoA hydrolase, HIBYL-CoA-H, 3-hydroxyisobutyryl-CoA hydrolase, 3-hydroxyisobutyryl-coenzyme A hydrolase
SwissProt ID
A2VDC2 (Xenla), P28817 (Yeast), Q2HJ73 (Bovin), Q5XIE6 (Rat), Q5ZJ60 (Chick), Q6NVY1 (Human), Q8QZS1 (Mouse)
Ncbi ID
9606, NP_055177, NP_055177.2, NP_666220.1, NM_146108, NM_001013112, NM_198047
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of HIBCH antibody
- Kit Component:
- - KN307704G1, Hibch gRNA vector 1 in pCas-Guide vector
- KN307704G2, Hibch gRNA vector 2 in pCas-Guide vector
- KN307704D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hendrickson,S.L. et al. (2010) PLoS ONE 5 (9), E12862Wu,C. et al. (2007) Proteomics 7 (11), 1775-1785
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIBCH antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of HIBCH antibody (Center) in HepG2 cell line lysates (35ug/lane). HIBCH (arrow) was detected using the purified Pab.
Anti-HIBCH mouse monoclonal antibody (TA501316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501316), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HIBCH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501317, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HIBCH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501317, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HIBCH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501317, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501317), and then analyzed by flow cytometry.
Anti-HIBCH mouse monoclonal antibody (TA501318) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501318), and then analyzed by flow cytometry.
Anti-HIBCH mouse monoclonal antibody (TA501319) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501319), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-HIBCH antibody(TA501319),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-HIBCH mouse monoclonal antibody (TA501320) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501320), and then analyzed by flow cytometry.
Anti-HIBCH mouse monoclonal antibody (TA501330) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501330), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-HIBCH antibody(TA501330),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-HIBCH mouse monoclonal antibody (TA501331) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501331), and then analyzed by flow cytometry.
Anti-HIBCH mouse monoclonal antibody (TA501332) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501332), and then analyzed by flow cytometry.
Anti-HIBCH mouse monoclonal antibody (TA501361) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501361), and then analyzed by flow cytometry.
Anti-HIBCH mouse monoclonal antibody (TA501387) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501387), and then analyzed by flow cytometry.
- Quick-Filters:
- Highly Validated (10)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA501316 | HIBCH antibody |
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| anti-HIBCH mouse monoclonal antibody, clone OTI3B10 (formerly 3B10) | |||||||
 |
Mouse | IgG1 | OTI3B10 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA501317 | HIBCH antibody |
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| anti-HIBCH mouse monoclonal antibody, clone OTI3H5 (formerly 3H5) | |||||||
 |
Mouse | IgG2a | OTI3H5 | Purified | Can, Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA501318 | HIBCH antibody |
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| anti-HIBCH mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) | |||||||
 |
Mouse | IgG1 | OTI2A5 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA501319 | HIBCH antibody |
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| HIBCH mouse monoclonal antibody, clone OTI1C7 (formerly 1C7) | |||||||
 |
Mouse | IgG2a | OTI1C7 | Purified | Can, Hu, Rt | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA501320 | HIBCH antibody |
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| anti-HIBCH mouse monoclonal antibody, clone OTI3E7 (formerly 3E7) | |||||||
 |
Mouse | IgG2b | OTI3E7 | Purified | Hu, Rt | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA501330 | HIBCH antibody |
||||||
| HIBCH mouse monoclonal antibody, clone OTI4G1 (formerly 4G1) | |||||||
 |
Mouse | IgG2b | OTI4G1 | Purified | Can, Hu, Mky, Rt | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA501331 | HIBCH antibody |
||||||
| anti-HIBCH mouse monoclonal antibody, clone OTI3G1 (formerly 3G1) | |||||||
 |
Mouse | IgG2b | OTI3G1 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA501332 | HIBCH antibody |
||||||
| anti-HIBCH mouse monoclonal antibody, clone OTI3F6 (formerly 3F6) | |||||||
 |
Mouse | IgG1 | OTI3F6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA501361 | HIBCH antibody |
||||||
| anti-HIBCH mouse monoclonal antibody, clone OTI5C5 (formerly 5C5) | |||||||
 |
Mouse | IgG1 | OTI5C5 | Purified | Can, Hu, Rt | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA501387 | HIBCH antibody |
||||||
| anti-HIBCH mouse monoclonal antibody, clone OTI3D11 (formerly 3D11) | |||||||
 |
Mouse | IgG1 | OTI3D11 | Purified | Can, Hu, Mky, Rt | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| AP14082PU-N | HIBCH antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309814 | HIBCH (transcript variant 1) |
||||
| Recombinant protein of human 3-hydroxyisobutyryl-Coenzyme A hydrolase (HIBCH), nuclear gene encoding mitochondrial protein, transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206019L2V | Lenti ORF particles, Hibch (GFP-tagged) - Mouse 3-hydroxyisobutyryl-Coenzyme A hydrolase (Hibch), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206019L1V | Lenti ORF particles, Hibch (Myc-DDK-tagged) - Mouse 3-hydroxyisobutyryl-Coenzyme A hydrolase (Hibch), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211069L2V | Lenti ORF particles, Hibch (GFP-tagged ORF) - Rat 3-hydroxyisobutyryl-Coenzyme A hydrolase (Hibch), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR211069L1V | Lenti ORF particles, Hibch (Myc-DDK-tagged ORF) - Rat 3-hydroxyisobutyryl-Coenzyme A hydrolase (Hibch), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||